QTL analysis of spike traits and KASP marker validation for basal sterile spikelet number in wheat
Abstract Spike traits play a pivotal role in determining wheat yield. In this study, two recombinant inbred line (RIL) populations were employed to identify quantitative trait loci (QTLs) for spike length (SL), spikelet number per spike (SNS), and basal sterile spikelet number (BSSN). A total of 30...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-04-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-025-06488-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850201195722833920 |
|---|---|
| author | Pengbo Song Yueyue Li Kefeng Song Yuanhang Xing Aoyan Zhang Wensha Zhao Huiling Zhao Xiaoran Guo Xin Zhang Shengjie Sun Yi Feng Daojie Sun |
| author_facet | Pengbo Song Yueyue Li Kefeng Song Yuanhang Xing Aoyan Zhang Wensha Zhao Huiling Zhao Xiaoran Guo Xin Zhang Shengjie Sun Yi Feng Daojie Sun |
| author_sort | Pengbo Song |
| collection | DOAJ |
| description | Abstract Spike traits play a pivotal role in determining wheat yield. In this study, two recombinant inbred line (RIL) populations were employed to identify quantitative trait loci (QTLs) for spike length (SL), spikelet number per spike (SNS), and basal sterile spikelet number (BSSN). A total of 30 QTLs were identified, including 7 major-effect QTLs that exhibited stability across multiple environments. Among these, two novel QTLs were discovered: QBssn.A-3 A.3 and QBssn.AV-5B.2. Furthermore, it was found that the combination of these two QTLs with other major effect QTLs had no impact on thousand-kernel weight (TKW). For QBssn.A-3 A.3, a Kompetitive Allele-Specific PCR (KASP) marker was developed and validated based on its physical location. Additionally, candidate genes within the intervals of QBssn.A-3 A.3 and QBssn.AV-5B.2 were predicted, based on these genes primarily associated with sterile spikelet number per spike. This study provides a foundation for future map-baesd cloning of genes related to spike traits, and for the breeding of high-yield wheat varieties. |
| format | Article |
| id | doaj-art-c4a7bd53ae394ceca37ea711c0a94f3a |
| institution | OA Journals |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-c4a7bd53ae394ceca37ea711c0a94f3a2025-08-20T02:12:06ZengBMCBMC Plant Biology1471-22292025-04-0125111210.1186/s12870-025-06488-9QTL analysis of spike traits and KASP marker validation for basal sterile spikelet number in wheatPengbo Song0Yueyue Li1Kefeng Song2Yuanhang Xing3Aoyan Zhang4Wensha Zhao5Huiling Zhao6Xiaoran Guo7Xin Zhang8Shengjie Sun9Yi Feng10Daojie Sun11College of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityCollege of Agronomy, Northwest A&F UniversityAbstract Spike traits play a pivotal role in determining wheat yield. In this study, two recombinant inbred line (RIL) populations were employed to identify quantitative trait loci (QTLs) for spike length (SL), spikelet number per spike (SNS), and basal sterile spikelet number (BSSN). A total of 30 QTLs were identified, including 7 major-effect QTLs that exhibited stability across multiple environments. Among these, two novel QTLs were discovered: QBssn.A-3 A.3 and QBssn.AV-5B.2. Furthermore, it was found that the combination of these two QTLs with other major effect QTLs had no impact on thousand-kernel weight (TKW). For QBssn.A-3 A.3, a Kompetitive Allele-Specific PCR (KASP) marker was developed and validated based on its physical location. Additionally, candidate genes within the intervals of QBssn.A-3 A.3 and QBssn.AV-5B.2 were predicted, based on these genes primarily associated with sterile spikelet number per spike. This study provides a foundation for future map-baesd cloning of genes related to spike traits, and for the breeding of high-yield wheat varieties.https://doi.org/10.1186/s12870-025-06488-9Wheat (Triticum aestivum L.)Basal sterile spikelet numberQTLKASP marker |
| spellingShingle | Pengbo Song Yueyue Li Kefeng Song Yuanhang Xing Aoyan Zhang Wensha Zhao Huiling Zhao Xiaoran Guo Xin Zhang Shengjie Sun Yi Feng Daojie Sun QTL analysis of spike traits and KASP marker validation for basal sterile spikelet number in wheat BMC Plant Biology Wheat (Triticum aestivum L.) Basal sterile spikelet number QTL KASP marker |
| title | QTL analysis of spike traits and KASP marker validation for basal sterile spikelet number in wheat |
| title_full | QTL analysis of spike traits and KASP marker validation for basal sterile spikelet number in wheat |
| title_fullStr | QTL analysis of spike traits and KASP marker validation for basal sterile spikelet number in wheat |
| title_full_unstemmed | QTL analysis of spike traits and KASP marker validation for basal sterile spikelet number in wheat |
| title_short | QTL analysis of spike traits and KASP marker validation for basal sterile spikelet number in wheat |
| title_sort | qtl analysis of spike traits and kasp marker validation for basal sterile spikelet number in wheat |
| topic | Wheat (Triticum aestivum L.) Basal sterile spikelet number QTL KASP marker |
| url | https://doi.org/10.1186/s12870-025-06488-9 |
| work_keys_str_mv | AT pengbosong qtlanalysisofspiketraitsandkaspmarkervalidationforbasalsterilespikeletnumberinwheat AT yueyueli qtlanalysisofspiketraitsandkaspmarkervalidationforbasalsterilespikeletnumberinwheat AT kefengsong qtlanalysisofspiketraitsandkaspmarkervalidationforbasalsterilespikeletnumberinwheat AT yuanhangxing qtlanalysisofspiketraitsandkaspmarkervalidationforbasalsterilespikeletnumberinwheat AT aoyanzhang qtlanalysisofspiketraitsandkaspmarkervalidationforbasalsterilespikeletnumberinwheat AT wenshazhao qtlanalysisofspiketraitsandkaspmarkervalidationforbasalsterilespikeletnumberinwheat AT huilingzhao qtlanalysisofspiketraitsandkaspmarkervalidationforbasalsterilespikeletnumberinwheat AT xiaoranguo qtlanalysisofspiketraitsandkaspmarkervalidationforbasalsterilespikeletnumberinwheat AT xinzhang qtlanalysisofspiketraitsandkaspmarkervalidationforbasalsterilespikeletnumberinwheat AT shengjiesun qtlanalysisofspiketraitsandkaspmarkervalidationforbasalsterilespikeletnumberinwheat AT yifeng qtlanalysisofspiketraitsandkaspmarkervalidationforbasalsterilespikeletnumberinwheat AT daojiesun qtlanalysisofspiketraitsandkaspmarkervalidationforbasalsterilespikeletnumberinwheat |