Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors
To identify materials suitable as membrane supports for ion channel biosensors, six filter materials of varying hydrophobicity, tortuosity, and thickness were examined for their ability to support bilayer lipid membranes as determined by electrical impedance spectroscopy. Bilayers supported by hydro...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Electrochemistry |
| Online Access: | http://dx.doi.org/10.4061/2011/213107 |
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| _version_ | 1832554921815179264 |
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| author | Thai Phung Yanli Zhang James Dunlop Julie E. Dalziel |
| author_facet | Thai Phung Yanli Zhang James Dunlop Julie E. Dalziel |
| author_sort | Thai Phung |
| collection | DOAJ |
| description | To identify materials suitable as membrane supports for ion channel biosensors, six filter materials of varying hydrophobicity, tortuosity, and thickness were examined for their ability to support bilayer lipid membranes as determined by electrical impedance spectroscopy. Bilayers supported by hydrophobic materials (PTFE, polycarbonate, nylon, and silanised silver) had optimal resistance (14–19 GΩ) and capacitance (0.8–1.6 μF) values whereas those with low hydrophobicity did not form BLMs (PVDF) or were short-lived (unsilanised silver). The ability of ion channels to function in BLMs was assessed using a method recently reported to improve the efficiency of proteoliposome incorporation into PTFE-supported bilayers. Voltage-gated sodium channel activation by veratridine and inhibition by saxitoxin showed activity for PTFE, nylon, and silanised silver, but not polycarbonate. Bilayers on thicker, more tortuous, and hydrophobic materials produced higher current levels. Bilayers that self-assembled on PTFE filters were the longest lived and produced the most channel activity using this method. |
| format | Article |
| id | doaj-art-c4a70ae9234e496fa56d560885b592c0 |
| institution | Kabale University |
| issn | 2090-3537 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Electrochemistry |
| spelling | doaj-art-c4a70ae9234e496fa56d560885b592c02025-02-03T05:50:07ZengWileyInternational Journal of Electrochemistry2090-35372011-01-01201110.4061/2011/213107213107Porous Materials to Support Bilayer Lipid Membranes for Ion Channel BiosensorsThai Phung0Yanli Zhang1James Dunlop2Julie E. Dalziel3AgResearch, Grasslands Research Centre, Tennent Drive, Private Bag 11008, Palmerston North 4442, New ZealandAgResearch, Grasslands Research Centre, Tennent Drive, Private Bag 11008, Palmerston North 4442, New ZealandMacDiarmid Institute for Advanced Materials and Nanotechnology, Victoria University of Wellington, P.O. Box 600, Wellington 6140, New ZealandAgResearch, Grasslands Research Centre, Tennent Drive, Private Bag 11008, Palmerston North 4442, New ZealandTo identify materials suitable as membrane supports for ion channel biosensors, six filter materials of varying hydrophobicity, tortuosity, and thickness were examined for their ability to support bilayer lipid membranes as determined by electrical impedance spectroscopy. Bilayers supported by hydrophobic materials (PTFE, polycarbonate, nylon, and silanised silver) had optimal resistance (14–19 GΩ) and capacitance (0.8–1.6 μF) values whereas those with low hydrophobicity did not form BLMs (PVDF) or were short-lived (unsilanised silver). The ability of ion channels to function in BLMs was assessed using a method recently reported to improve the efficiency of proteoliposome incorporation into PTFE-supported bilayers. Voltage-gated sodium channel activation by veratridine and inhibition by saxitoxin showed activity for PTFE, nylon, and silanised silver, but not polycarbonate. Bilayers on thicker, more tortuous, and hydrophobic materials produced higher current levels. Bilayers that self-assembled on PTFE filters were the longest lived and produced the most channel activity using this method.http://dx.doi.org/10.4061/2011/213107 |
| spellingShingle | Thai Phung Yanli Zhang James Dunlop Julie E. Dalziel Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors International Journal of Electrochemistry |
| title | Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors |
| title_full | Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors |
| title_fullStr | Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors |
| title_full_unstemmed | Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors |
| title_short | Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors |
| title_sort | porous materials to support bilayer lipid membranes for ion channel biosensors |
| url | http://dx.doi.org/10.4061/2011/213107 |
| work_keys_str_mv | AT thaiphung porousmaterialstosupportbilayerlipidmembranesforionchannelbiosensors AT yanlizhang porousmaterialstosupportbilayerlipidmembranesforionchannelbiosensors AT jamesdunlop porousmaterialstosupportbilayerlipidmembranesforionchannelbiosensors AT julieedalziel porousmaterialstosupportbilayerlipidmembranesforionchannelbiosensors |