A Subunit Vaccine Harboring the Fusion Capsid Proteins of Porcine Circovirus Types 2, 3, and 4 Induces Protective Immune Responses in a Mouse Model
Coinfections with porcine circovirus types 2, 3, and 4 (PCV2, PCV3, and PCV4) are increasingly being detected in the swine industry. However, there is no commercially available vaccine which prevents coinfection with PCV2, PCV3, and PCV4. The development of a vaccine expressing capsid (Cap) fusion p...
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MDPI AG
2024-12-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/16/12/1964 |
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| author | Qikai Wang Ran Zhang Yue Wang Ying Wang Libin Liang Haili Ma Haidong Wang Longlong Si Xingchen Wu |
| author_facet | Qikai Wang Ran Zhang Yue Wang Ying Wang Libin Liang Haili Ma Haidong Wang Longlong Si Xingchen Wu |
| author_sort | Qikai Wang |
| collection | DOAJ |
| description | Coinfections with porcine circovirus types 2, 3, and 4 (PCV2, PCV3, and PCV4) are increasingly being detected in the swine industry. However, there is no commercially available vaccine which prevents coinfection with PCV2, PCV3, and PCV4. The development of a vaccine expressing capsid (Cap) fusion proteins of multiple PCVs represents a promising approach for broadly preventing infection with PCVs. In this study, we developed a PCV subunit vaccine candidate (Cap 2-3-4) by predicting, screening, and fusing antigenic epitopes of Cap proteins of PCV2, PCV3, and PCV4. Immunoprotection assays showed that the prokaryotic expression of Cap 2-3-4 could effectively induce high levels of PCV2, PCV3, and PCV4 Cap-specific antibodies and successfully neutralize both PCV2 and PCV3. Furthermore, Cap 2-3-4 demonstrated a potent ability to activate cellular immunity and thus prevent lung damage in mice. This study provides a new option for the development of broad vaccines against PCVs. |
| format | Article |
| id | doaj-art-c49e3a9aae7843b5b0f4a42af0ca2acc |
| institution | DOAJ |
| issn | 1999-4915 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-c49e3a9aae7843b5b0f4a42af0ca2acc2025-08-20T02:43:49ZengMDPI AGViruses1999-49152024-12-011612196410.3390/v16121964A Subunit Vaccine Harboring the Fusion Capsid Proteins of Porcine Circovirus Types 2, 3, and 4 Induces Protective Immune Responses in a Mouse ModelQikai Wang0Ran Zhang1Yue Wang2Ying Wang3Libin Liang4Haili Ma5Haidong Wang6Longlong Si7Xingchen Wu8College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaKey Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaCollege of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, ChinaCoinfections with porcine circovirus types 2, 3, and 4 (PCV2, PCV3, and PCV4) are increasingly being detected in the swine industry. However, there is no commercially available vaccine which prevents coinfection with PCV2, PCV3, and PCV4. The development of a vaccine expressing capsid (Cap) fusion proteins of multiple PCVs represents a promising approach for broadly preventing infection with PCVs. In this study, we developed a PCV subunit vaccine candidate (Cap 2-3-4) by predicting, screening, and fusing antigenic epitopes of Cap proteins of PCV2, PCV3, and PCV4. Immunoprotection assays showed that the prokaryotic expression of Cap 2-3-4 could effectively induce high levels of PCV2, PCV3, and PCV4 Cap-specific antibodies and successfully neutralize both PCV2 and PCV3. Furthermore, Cap 2-3-4 demonstrated a potent ability to activate cellular immunity and thus prevent lung damage in mice. This study provides a new option for the development of broad vaccines against PCVs.https://www.mdpi.com/1999-4915/16/12/1964porcine circoviruscapsidantigenic epitopefusion proteinsubunit vaccineimmunogenicity |
| spellingShingle | Qikai Wang Ran Zhang Yue Wang Ying Wang Libin Liang Haili Ma Haidong Wang Longlong Si Xingchen Wu A Subunit Vaccine Harboring the Fusion Capsid Proteins of Porcine Circovirus Types 2, 3, and 4 Induces Protective Immune Responses in a Mouse Model Viruses porcine circovirus capsid antigenic epitope fusion protein subunit vaccine immunogenicity |
| title | A Subunit Vaccine Harboring the Fusion Capsid Proteins of Porcine Circovirus Types 2, 3, and 4 Induces Protective Immune Responses in a Mouse Model |
| title_full | A Subunit Vaccine Harboring the Fusion Capsid Proteins of Porcine Circovirus Types 2, 3, and 4 Induces Protective Immune Responses in a Mouse Model |
| title_fullStr | A Subunit Vaccine Harboring the Fusion Capsid Proteins of Porcine Circovirus Types 2, 3, and 4 Induces Protective Immune Responses in a Mouse Model |
| title_full_unstemmed | A Subunit Vaccine Harboring the Fusion Capsid Proteins of Porcine Circovirus Types 2, 3, and 4 Induces Protective Immune Responses in a Mouse Model |
| title_short | A Subunit Vaccine Harboring the Fusion Capsid Proteins of Porcine Circovirus Types 2, 3, and 4 Induces Protective Immune Responses in a Mouse Model |
| title_sort | subunit vaccine harboring the fusion capsid proteins of porcine circovirus types 2 3 and 4 induces protective immune responses in a mouse model |
| topic | porcine circovirus capsid antigenic epitope fusion protein subunit vaccine immunogenicity |
| url | https://www.mdpi.com/1999-4915/16/12/1964 |
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