State-of-the-art signal amplification strategies for nucleic acid and non-nucleic acid biosensors
Biosensors have garnered substantial attention as an emerging toolbox for analyzing biomarkers, physiological processes, food, and environmental metrices. Rapid, sensitive, and selective detection is pivotal to the practical utility of a biosensing system. Over the last few years, strides have been...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Sensors and Actuators Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666053924000845 |
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| author | Ijaz Gul Muhammad Akmal Raheem Md. Reyad-ul-Ferdous Xi Yuan Zhenglin Chen Chenying lv Minjiang Chen Jiansong Ji Duanpo Wu Qiang Zhao Chenggang Yan Dongmei Yu |
| author_facet | Ijaz Gul Muhammad Akmal Raheem Md. Reyad-ul-Ferdous Xi Yuan Zhenglin Chen Chenying lv Minjiang Chen Jiansong Ji Duanpo Wu Qiang Zhao Chenggang Yan Dongmei Yu |
| author_sort | Ijaz Gul |
| collection | DOAJ |
| description | Biosensors have garnered substantial attention as an emerging toolbox for analyzing biomarkers, physiological processes, food, and environmental metrices. Rapid, sensitive, and selective detection is pivotal to the practical utility of a biosensing system. Over the last few years, strides have been made in biosensing research to develop next-generation sensing systems that meet WHO's ASSURED criteria. Shorter response times, accuracy, high sensitivity, and selectivity are immensely important parameters of a biosensor, directly impacted by the biosensing signal generation and detection modality. Herein, we critically analyze the state-of-the-art signal amplification strategies for various nucleic acid and non-nucleic acid bioanalytes to improve the analytical performance of biosensing systems. We first provide a brief overview of the key components of biosensors. Next, we provide insights on state-of-the-art signal amplification strategies by dividing them into target preamplification, target enrichment, cascade reactions, biocomponent engineering, exploring functional materials, optimizing reaction conditions, designing novel microfluidic devices, and harnessing AI, along with selected examples. We evaluate the development trends of these signal enhancement approaches and discuss pros and cons of each approach. Finally, we highlight current challenges and future considerations for this emerging interdisciplinary research area. |
| format | Article |
| id | doaj-art-c48fc079ca484bd9bab05bc07781fe37 |
| institution | OA Journals |
| issn | 2666-0539 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensors and Actuators Reports |
| spelling | doaj-art-c48fc079ca484bd9bab05bc07781fe372025-08-20T01:56:49ZengElsevierSensors and Actuators Reports2666-05392025-06-01910026810.1016/j.snr.2024.100268State-of-the-art signal amplification strategies for nucleic acid and non-nucleic acid biosensorsIjaz Gul0Muhammad Akmal Raheem1Md. Reyad-ul-Ferdous2Xi Yuan3Zhenglin Chen4Chenying lv5Minjiang Chen6Jiansong Ji7Duanpo Wu8Qiang Zhao9Chenggang Yan10Dongmei Yu11Lishui Institute of Hangzhou Dianzi University, Lishui, PR China; Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, PR China; School of Communication Engineering, Hangzhou Dianzi University, Hangzhou, PR ChinaInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, PR ChinaInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, PR ChinaLishui Institute of Hangzhou Dianzi University, Lishui, PR China; Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, PR China; School of Communication Engineering, Hangzhou Dianzi University, Hangzhou, PR ChinaZhejiang Key Laboratory of Imaging and Interventional Medicine, Zhejiang Engineering Research Center of Interventional Medicine Engineering and Biotechnology, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui 323000, PR ChinaZhejiang Key Laboratory of Imaging and Interventional Medicine, Zhejiang Engineering Research Center of Interventional Medicine Engineering and Biotechnology, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui 323000, PR ChinaZhejiang Key Laboratory of Imaging and Interventional Medicine, Zhejiang Engineering Research Center of Interventional Medicine Engineering and Biotechnology, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui 323000, PR ChinaZhejiang Key Laboratory of Imaging and Interventional Medicine, Zhejiang Engineering Research Center of Interventional Medicine Engineering and Biotechnology, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui 323000, PR ChinaLishui Institute of Hangzhou Dianzi University, Lishui, PR China; School of Communication Engineering, Hangzhou Dianzi University, Hangzhou, PR ChinaLishui Institute of Hangzhou Dianzi University, Lishui, PR China; School of Communication Engineering, Hangzhou Dianzi University, Hangzhou, PR ChinaLishui Institute of Hangzhou Dianzi University, Lishui, PR China; School of Communication Engineering, Hangzhou Dianzi University, Hangzhou, PR ChinaLishui Institute of Hangzhou Dianzi University, Lishui, PR China; School of Communication Engineering, Hangzhou Dianzi University, Hangzhou, PR China; School of Mechanical, Electrical & Information Engineering, Shandong University, Weihai, Shandong, PR China; Corresponding author at: School of Mechanical, Electrical & Information Engineering, Shandong University, Weihai, Shandong, PR China.Biosensors have garnered substantial attention as an emerging toolbox for analyzing biomarkers, physiological processes, food, and environmental metrices. Rapid, sensitive, and selective detection is pivotal to the practical utility of a biosensing system. Over the last few years, strides have been made in biosensing research to develop next-generation sensing systems that meet WHO's ASSURED criteria. Shorter response times, accuracy, high sensitivity, and selectivity are immensely important parameters of a biosensor, directly impacted by the biosensing signal generation and detection modality. Herein, we critically analyze the state-of-the-art signal amplification strategies for various nucleic acid and non-nucleic acid bioanalytes to improve the analytical performance of biosensing systems. We first provide a brief overview of the key components of biosensors. Next, we provide insights on state-of-the-art signal amplification strategies by dividing them into target preamplification, target enrichment, cascade reactions, biocomponent engineering, exploring functional materials, optimizing reaction conditions, designing novel microfluidic devices, and harnessing AI, along with selected examples. We evaluate the development trends of these signal enhancement approaches and discuss pros and cons of each approach. Finally, we highlight current challenges and future considerations for this emerging interdisciplinary research area.http://www.sciencedirect.com/science/article/pii/S2666053924000845Signal amplificationCRISPRBiosensorsBioreceptorNon-nucleic acid |
| spellingShingle | Ijaz Gul Muhammad Akmal Raheem Md. Reyad-ul-Ferdous Xi Yuan Zhenglin Chen Chenying lv Minjiang Chen Jiansong Ji Duanpo Wu Qiang Zhao Chenggang Yan Dongmei Yu State-of-the-art signal amplification strategies for nucleic acid and non-nucleic acid biosensors Sensors and Actuators Reports Signal amplification CRISPR Biosensors Bioreceptor Non-nucleic acid |
| title | State-of-the-art signal amplification strategies for nucleic acid and non-nucleic acid biosensors |
| title_full | State-of-the-art signal amplification strategies for nucleic acid and non-nucleic acid biosensors |
| title_fullStr | State-of-the-art signal amplification strategies for nucleic acid and non-nucleic acid biosensors |
| title_full_unstemmed | State-of-the-art signal amplification strategies for nucleic acid and non-nucleic acid biosensors |
| title_short | State-of-the-art signal amplification strategies for nucleic acid and non-nucleic acid biosensors |
| title_sort | state of the art signal amplification strategies for nucleic acid and non nucleic acid biosensors |
| topic | Signal amplification CRISPR Biosensors Bioreceptor Non-nucleic acid |
| url | http://www.sciencedirect.com/science/article/pii/S2666053924000845 |
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