High resistance prevalence towards ampicillin, co-trimoxazole and ciprofloxacin, among uropathogenic Escherichia coli isolates in Mexico City

Background : The prevalence of antimicrobial resistance among uropathogenic E. coli varies widely worldwide; to guide empirical therapy is necessary to have local, up-to-date susceptibility data. Methodology: We tested 907 isolates from patients in Mexico City by disk diffusion and further charact...

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Main Authors: Jose Luis Arredondo-García, Carlos F. Amábile-Cuevas
Format: Article
Language:English
Published: The Journal of Infection in Developing Countries 2008-10-01
Series:Journal of Infection in Developing Countries
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Online Access:https://jidc.org/index.php/journal/article/view/195
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author Jose Luis Arredondo-García
Carlos F. Amábile-Cuevas
author_facet Jose Luis Arredondo-García
Carlos F. Amábile-Cuevas
author_sort Jose Luis Arredondo-García
collection DOAJ
description Background : The prevalence of antimicrobial resistance among uropathogenic E. coli varies widely worldwide; to guide empirical therapy is necessary to have local, up-to-date susceptibility data. Methodology: We tested 907 isolates from patients in Mexico City by disk diffusion and further characterized ciprofloxacin, cephalosporin and nitrofurantoin resistant strains. Results: Isolates were mostly resistant to ampicillin (74%), trimethoprim-sulfamethoxazole (60.1%) and ciprofloxacin (32.6%). The most effective drug was netilmicin (5.1% resistant) and the most effective of oral drugs was nitrofurantoin (7.4% resistant). Sixty-percent of ciprofloxacin-resistant strains had minimal inhibitory concentrations of 125 μg/ml or higher, well beyond urinary concentrations at the end of the 12-hour inter-dose period for standard oral regimes. Extended-spectrum beta-lactamases were detected in 6% of strains, most of them from community-acquired infections. All strains resistant to nitrofurantoin carried a ~20 Kb plasmid, which when transformed into a susceptible recipient, conferred resistance to nitrofurantoin, ampicillin, sulfonamides, streptomycin, and partially protected against ciprofloxacin. Conclusions: Drugs considered of choice against uncomplicated urinary tract infections are facing high resistance prevalences and resistance determinants formerly seen only at hospitals are now among community strains. Treatment guidelines from developed countries might not reflect these local trends.
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spelling doaj-art-c48663963a1f4b59b16de736c66ddaca2025-08-20T02:16:07ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802008-10-0120510.3855/jidc.195High resistance prevalence towards ampicillin, co-trimoxazole and ciprofloxacin, among uropathogenic Escherichia coli isolates in Mexico CityJose Luis Arredondo-García0Carlos F. Amábile-Cuevas1Instituto Nacional de Pediatría, Mexico CityFundación Lusara, Mexico CityBackground : The prevalence of antimicrobial resistance among uropathogenic E. coli varies widely worldwide; to guide empirical therapy is necessary to have local, up-to-date susceptibility data. Methodology: We tested 907 isolates from patients in Mexico City by disk diffusion and further characterized ciprofloxacin, cephalosporin and nitrofurantoin resistant strains. Results: Isolates were mostly resistant to ampicillin (74%), trimethoprim-sulfamethoxazole (60.1%) and ciprofloxacin (32.6%). The most effective drug was netilmicin (5.1% resistant) and the most effective of oral drugs was nitrofurantoin (7.4% resistant). Sixty-percent of ciprofloxacin-resistant strains had minimal inhibitory concentrations of 125 μg/ml or higher, well beyond urinary concentrations at the end of the 12-hour inter-dose period for standard oral regimes. Extended-spectrum beta-lactamases were detected in 6% of strains, most of them from community-acquired infections. All strains resistant to nitrofurantoin carried a ~20 Kb plasmid, which when transformed into a susceptible recipient, conferred resistance to nitrofurantoin, ampicillin, sulfonamides, streptomycin, and partially protected against ciprofloxacin. Conclusions: Drugs considered of choice against uncomplicated urinary tract infections are facing high resistance prevalences and resistance determinants formerly seen only at hospitals are now among community strains. Treatment guidelines from developed countries might not reflect these local trends.https://jidc.org/index.php/journal/article/view/195uropathogenic E. coliampicillinco-trimoxazoleciprofloxacinnitrofurantoinantibiotic resistance
spellingShingle Jose Luis Arredondo-García
Carlos F. Amábile-Cuevas
High resistance prevalence towards ampicillin, co-trimoxazole and ciprofloxacin, among uropathogenic Escherichia coli isolates in Mexico City
Journal of Infection in Developing Countries
uropathogenic E. coli
ampicillin
co-trimoxazole
ciprofloxacin
nitrofurantoin
antibiotic resistance
title High resistance prevalence towards ampicillin, co-trimoxazole and ciprofloxacin, among uropathogenic Escherichia coli isolates in Mexico City
title_full High resistance prevalence towards ampicillin, co-trimoxazole and ciprofloxacin, among uropathogenic Escherichia coli isolates in Mexico City
title_fullStr High resistance prevalence towards ampicillin, co-trimoxazole and ciprofloxacin, among uropathogenic Escherichia coli isolates in Mexico City
title_full_unstemmed High resistance prevalence towards ampicillin, co-trimoxazole and ciprofloxacin, among uropathogenic Escherichia coli isolates in Mexico City
title_short High resistance prevalence towards ampicillin, co-trimoxazole and ciprofloxacin, among uropathogenic Escherichia coli isolates in Mexico City
title_sort high resistance prevalence towards ampicillin co trimoxazole and ciprofloxacin among uropathogenic escherichia coli isolates in mexico city
topic uropathogenic E. coli
ampicillin
co-trimoxazole
ciprofloxacin
nitrofurantoin
antibiotic resistance
url https://jidc.org/index.php/journal/article/view/195
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