Phytochemical Constituents and Antimicrobial Activity of Carica papaya Leaf Extract on Some Clinical Isolates

Herbal medicine has gained increasing usage globally quite recently, as a result of increasing drug resistance to conventional drugs. This has led to an increase in the search for new and effective sources of antimicrobials. This study assessed the antimicrobial efficacy of Carica papaya extract on...

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Main Author: Achukwu, N.O.1*, Aniobi, C.C.2, Isiofia, P.O.1, Ajare, C.C.1, Agu, A.N.1
Format: Article
Language:English
Published: Hammer Head Production Limited 2024-07-01
Series:Sokoto Journal of Medical Laboratory Science
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Online Access:https://sokjmls.com.ng/index.php/SJMLS/article/view/536
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author Achukwu, N.O.1*, Aniobi, C.C.2, Isiofia, P.O.1, Ajare, C.C.1, Agu, A.N.1
author_facet Achukwu, N.O.1*, Aniobi, C.C.2, Isiofia, P.O.1, Ajare, C.C.1, Agu, A.N.1
author_sort Achukwu, N.O.1*, Aniobi, C.C.2, Isiofia, P.O.1, Ajare, C.C.1, Agu, A.N.1
collection DOAJ
description Herbal medicine has gained increasing usage globally quite recently, as a result of increasing drug resistance to conventional drugs. This has led to an increase in the search for new and effective sources of antimicrobials. This study assessed the antimicrobial efficacy of Carica papaya extract on some clinical bacteria and fungi isolates and the phytochemical composition of the leaves. The clinical isolates used for this study were 5 bacterial isolates (Klebsiella pneumonia, Escherichia coli. Staphylococcus aureus, Streptococcus pyogenes and Pseudomonas aeruginosa) and 3 fungal isolates (Aspergillus niger, Candida albicans, and Aspergillus flavus) The leaf of Carica papaya was extracted using ethanol, and water. The antimicrobial activity was determined using the agar well diffusion technique. The extracts were prepared at a concentration of 200mg/ml and were used on both the bacteria and fungi isolates. The MIC was assessed using broth serial doubling dilution at different concentrations. Both extracts are active to the isolates at varying degrees. The MIC and MMC range between 50 -200mg/ml. The phytochemical screening showed that Carica papaya leaves contain essential secondary metabolites that aid in the antimicrobial activity. This study reasonably supports the use of Carica papaya leaves in the treatment of infections as used by traditional herbal practitioners.
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institution Kabale University
issn 2536-7153
language English
publishDate 2024-07-01
publisher Hammer Head Production Limited
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series Sokoto Journal of Medical Laboratory Science
spelling doaj-art-c484aa9869f04d9a9327bfc592216a3a2025-08-20T03:39:48ZengHammer Head Production LimitedSokoto Journal of Medical Laboratory Science2536-71532024-07-0192536Phytochemical Constituents and Antimicrobial Activity of Carica papaya Leaf Extract on Some Clinical IsolatesAchukwu, N.O.1*, Aniobi, C.C.2, Isiofia, P.O.1, Ajare, C.C.1, Agu, A.N.1Herbal medicine has gained increasing usage globally quite recently, as a result of increasing drug resistance to conventional drugs. This has led to an increase in the search for new and effective sources of antimicrobials. This study assessed the antimicrobial efficacy of Carica papaya extract on some clinical bacteria and fungi isolates and the phytochemical composition of the leaves. The clinical isolates used for this study were 5 bacterial isolates (Klebsiella pneumonia, Escherichia coli. Staphylococcus aureus, Streptococcus pyogenes and Pseudomonas aeruginosa) and 3 fungal isolates (Aspergillus niger, Candida albicans, and Aspergillus flavus) The leaf of Carica papaya was extracted using ethanol, and water. The antimicrobial activity was determined using the agar well diffusion technique. The extracts were prepared at a concentration of 200mg/ml and were used on both the bacteria and fungi isolates. The MIC was assessed using broth serial doubling dilution at different concentrations. Both extracts are active to the isolates at varying degrees. The MIC and MMC range between 50 -200mg/ml. The phytochemical screening showed that Carica papaya leaves contain essential secondary metabolites that aid in the antimicrobial activity. This study reasonably supports the use of Carica papaya leaves in the treatment of infections as used by traditional herbal practitioners.https://sokjmls.com.ng/index.php/SJMLS/article/view/536carica papaya; antimicrobial; clinical isolate; phytochemical; mic; mmc
spellingShingle Achukwu, N.O.1*, Aniobi, C.C.2, Isiofia, P.O.1, Ajare, C.C.1, Agu, A.N.1
Phytochemical Constituents and Antimicrobial Activity of Carica papaya Leaf Extract on Some Clinical Isolates
Sokoto Journal of Medical Laboratory Science
carica papaya; antimicrobial; clinical isolate; phytochemical; mic; mmc
title Phytochemical Constituents and Antimicrobial Activity of Carica papaya Leaf Extract on Some Clinical Isolates
title_full Phytochemical Constituents and Antimicrobial Activity of Carica papaya Leaf Extract on Some Clinical Isolates
title_fullStr Phytochemical Constituents and Antimicrobial Activity of Carica papaya Leaf Extract on Some Clinical Isolates
title_full_unstemmed Phytochemical Constituents and Antimicrobial Activity of Carica papaya Leaf Extract on Some Clinical Isolates
title_short Phytochemical Constituents and Antimicrobial Activity of Carica papaya Leaf Extract on Some Clinical Isolates
title_sort phytochemical constituents and antimicrobial activity of carica papaya leaf extract on some clinical isolates
topic carica papaya; antimicrobial; clinical isolate; phytochemical; mic; mmc
url https://sokjmls.com.ng/index.php/SJMLS/article/view/536
work_keys_str_mv AT achukwuno1aniobicc2isiofiapo1ajarecc1aguan1 phytochemicalconstituentsandantimicrobialactivityofcaricapapayaleafextractonsomeclinicalisolates