Elucidation of phenolic profile and bioactive potential of Aeginetia indica L.: A comparative study between newly discovered yellow and native purple flowers
Aeginetia indica L. is a wild plant, with scant information on its food and medicinal applications. This research investigated the phenolics, antioxidant properties, in vitro medicinal activities, and genotoxicity of the newly discovered yellow and native purple flowers of A. indica collected from d...
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Elsevier
2025-08-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154325005290 |
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| author | Piya Temviriyanukul Sirinapa Thangsiri Woorawee Inthachat Nattira On–Nom Yuraporn Sahasakul Amornrat Aursalung Chaowanee Chupeerach Uthaiwan Suttisansanee |
| author_facet | Piya Temviriyanukul Sirinapa Thangsiri Woorawee Inthachat Nattira On–Nom Yuraporn Sahasakul Amornrat Aursalung Chaowanee Chupeerach Uthaiwan Suttisansanee |
| author_sort | Piya Temviriyanukul |
| collection | DOAJ |
| description | Aeginetia indica L. is a wild plant, with scant information on its food and medicinal applications. This research investigated the phenolics, antioxidant properties, in vitro medicinal activities, and genotoxicity of the newly discovered yellow and native purple flowers of A. indica collected from diverse locations in Thailand. Purple A. indica harvested from Ubon Ratchathani Province (PU) exhibited 1.3–1.4-fold higher total phenolic content (TPC) and 1.1–1.4-fold higher total flavonoid content (TFC) than purple A. indica collected from Sakon Nakhon Province (PS) and yellow A. indica collected from Sakon Nakhon Province (YS), with apigenin and naringenin predominantly detected in PU by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). The purple color of PU and PS led to 2.5–2.9-fold higher total anthocyanin content (TAC) than YS. Higher phenolic content in PU led to stronger antioxidant activities by 1.2–1.3- and 1.2–1.8-fold as determined by ferric ion reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assays, respectively, while the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was not significantly different from PS. PU and PS exhibited 1.1-fold lower scavenging capacities (50 % of total radicals or SC50) than YS, suggesting their higher antioxidant strength. Higher inhibitory activities of most key enzymes relevant to obesity (lipase, 1.6–3.3-fold higher), type II diabetes (α-amylase, 1.3–1.6-fold higher), and Alzheimer's disease (acetylcholinesterase (AChE, 1.6–3.0-fold higher) and butyrylcholinesterase (BChE, 1.1-fold higher)) were also observed in PU. Further investigation of the PU extract with orlistat (a commercially available anti-obesity drug) and donepezil (a commercially available anti-Alzheimer's disease drug) suggested synergistic effects of PU with both drugs. PU did not induce gene mutations as assayed by the bacterial reverse mutation test. The results suggested that PU could be further developed as a future functional food or health ingredient. |
| format | Article |
| id | doaj-art-c482b208ec664ca8a92e8d46819a6766 |
| institution | DOAJ |
| issn | 2666-1543 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
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| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-c482b208ec664ca8a92e8d46819a67662025-08-20T02:47:19ZengElsevierJournal of Agriculture and Food Research2666-15432025-08-012210215810.1016/j.jafr.2025.102158Elucidation of phenolic profile and bioactive potential of Aeginetia indica L.: A comparative study between newly discovered yellow and native purple flowersPiya Temviriyanukul0Sirinapa Thangsiri1Woorawee Inthachat2Nattira On–Nom3Yuraporn Sahasakul4Amornrat Aursalung5Chaowanee Chupeerach6Uthaiwan Suttisansanee7Food and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom, 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom, 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom, 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom, 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom, 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom, 73170, ThailandFood and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom, 73170, ThailandCorresponding author.; Food and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Phuttamonthon, Nakhon Pathom, 73170, ThailandAeginetia indica L. is a wild plant, with scant information on its food and medicinal applications. This research investigated the phenolics, antioxidant properties, in vitro medicinal activities, and genotoxicity of the newly discovered yellow and native purple flowers of A. indica collected from diverse locations in Thailand. Purple A. indica harvested from Ubon Ratchathani Province (PU) exhibited 1.3–1.4-fold higher total phenolic content (TPC) and 1.1–1.4-fold higher total flavonoid content (TFC) than purple A. indica collected from Sakon Nakhon Province (PS) and yellow A. indica collected from Sakon Nakhon Province (YS), with apigenin and naringenin predominantly detected in PU by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). The purple color of PU and PS led to 2.5–2.9-fold higher total anthocyanin content (TAC) than YS. Higher phenolic content in PU led to stronger antioxidant activities by 1.2–1.3- and 1.2–1.8-fold as determined by ferric ion reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assays, respectively, while the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was not significantly different from PS. PU and PS exhibited 1.1-fold lower scavenging capacities (50 % of total radicals or SC50) than YS, suggesting their higher antioxidant strength. Higher inhibitory activities of most key enzymes relevant to obesity (lipase, 1.6–3.3-fold higher), type II diabetes (α-amylase, 1.3–1.6-fold higher), and Alzheimer's disease (acetylcholinesterase (AChE, 1.6–3.0-fold higher) and butyrylcholinesterase (BChE, 1.1-fold higher)) were also observed in PU. Further investigation of the PU extract with orlistat (a commercially available anti-obesity drug) and donepezil (a commercially available anti-Alzheimer's disease drug) suggested synergistic effects of PU with both drugs. PU did not induce gene mutations as assayed by the bacterial reverse mutation test. The results suggested that PU could be further developed as a future functional food or health ingredient.http://www.sciencedirect.com/science/article/pii/S2666154325005290Alzheimer's diseaseDiabetesEnzyme inhibitionGenotoxicityHuman healthObesity |
| spellingShingle | Piya Temviriyanukul Sirinapa Thangsiri Woorawee Inthachat Nattira On–Nom Yuraporn Sahasakul Amornrat Aursalung Chaowanee Chupeerach Uthaiwan Suttisansanee Elucidation of phenolic profile and bioactive potential of Aeginetia indica L.: A comparative study between newly discovered yellow and native purple flowers Journal of Agriculture and Food Research Alzheimer's disease Diabetes Enzyme inhibition Genotoxicity Human health Obesity |
| title | Elucidation of phenolic profile and bioactive potential of Aeginetia indica L.: A comparative study between newly discovered yellow and native purple flowers |
| title_full | Elucidation of phenolic profile and bioactive potential of Aeginetia indica L.: A comparative study between newly discovered yellow and native purple flowers |
| title_fullStr | Elucidation of phenolic profile and bioactive potential of Aeginetia indica L.: A comparative study between newly discovered yellow and native purple flowers |
| title_full_unstemmed | Elucidation of phenolic profile and bioactive potential of Aeginetia indica L.: A comparative study between newly discovered yellow and native purple flowers |
| title_short | Elucidation of phenolic profile and bioactive potential of Aeginetia indica L.: A comparative study between newly discovered yellow and native purple flowers |
| title_sort | elucidation of phenolic profile and bioactive potential of aeginetia indica l a comparative study between newly discovered yellow and native purple flowers |
| topic | Alzheimer's disease Diabetes Enzyme inhibition Genotoxicity Human health Obesity |
| url | http://www.sciencedirect.com/science/article/pii/S2666154325005290 |
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