Deltamethrin Selection Drives Transcriptomic Changes in Detoxification, Immune, and Cuticle Genes in <i>Aedes aegypti</i>
The rapid global expansion of <i>Aedes aegypti</i>-borne diseases such as dengue, chikungunya, and Zika has positioned this mosquito as a key target for vector control programs. These programs rely heavily on insecticide use, leading to the widespread emergence of insecticide resistance....
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2025-06-01
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| Series: | Tropical Medicine and Infectious Disease |
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| author | Yamili Contreras-Perera Lucy Mackenzie-Impoinvil Dieunel Derilus Audrey Lenhart Iram P. Rodriguez-Sanchez Pablo Manrique-Saide Adriana E. Flores |
| author_facet | Yamili Contreras-Perera Lucy Mackenzie-Impoinvil Dieunel Derilus Audrey Lenhart Iram P. Rodriguez-Sanchez Pablo Manrique-Saide Adriana E. Flores |
| author_sort | Yamili Contreras-Perera |
| collection | DOAJ |
| description | The rapid global expansion of <i>Aedes aegypti</i>-borne diseases such as dengue, chikungunya, and Zika has positioned this mosquito as a key target for vector control programs. These programs rely heavily on insecticide use, leading to the widespread emergence of insecticide resistance. Understanding the molecular basis of resistance is essential for developing effective management strategies. In this study, we employed a whole-transcriptome (RNA-seq) approach to analyze gene expression in three <i>Ae. aegypti</i> populations from Mexico that underwent four generations of laboratory selection with deltamethrin. Several cytochrome P450 genes (CYP6AG4, CYP6M5, CYP307A1) and a chitin-binding peritrophin-like gene (Ae-Aper50) were significantly overexpressed following selection, supporting roles for both detoxification and midgut protection. We also observed a consistent downregulation of cuticular protein genes in deltamethrin-selected groups relative to the baseline populations, suggesting their involvement in baseline tolerance rather than induced resistance. Additionally, the overexpression of immune- and stress-related genes, including the RNA helicase MOV-10, indicates that insecticide selection may trigger broader physiological responses. These findings highlight complex, multi-pathway transcriptomic changes associated with resistance development in <i>Ae. aegypti</i>. |
| format | Article |
| id | doaj-art-c473a88325a64ae98f8d7e955de9745e |
| institution | OA Journals |
| issn | 2414-6366 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Tropical Medicine and Infectious Disease |
| spelling | doaj-art-c473a88325a64ae98f8d7e955de9745e2025-08-20T02:21:51ZengMDPI AGTropical Medicine and Infectious Disease2414-63662025-06-0110617110.3390/tropicalmed10060171Deltamethrin Selection Drives Transcriptomic Changes in Detoxification, Immune, and Cuticle Genes in <i>Aedes aegypti</i>Yamili Contreras-Perera0Lucy Mackenzie-Impoinvil1Dieunel Derilus2Audrey Lenhart3Iram P. Rodriguez-Sanchez4Pablo Manrique-Saide5Adriana E. Flores6Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, Av. Universidad s/n, Cd. Universitaria, San Nicolas de los Garza 66451, MexicoEntomology Branch, Division of Parasitic Diseases and Malaria, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30329, USAEntomology Branch, Division of Parasitic Diseases and Malaria, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30329, USAEntomology Branch, Division of Parasitic Diseases and Malaria, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd, Atlanta, GA 30329, USAFacultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, Av. Universidad s/n, Cd. Universitaria, San Nicolas de los Garza 66451, MexicoLaboratorio para el Control Biológico de Aedes aegypti (LCB-UADY), Unidad Colaborativa para Bioensayos Entomologicos, Campus de Ciencias Biologicas y Agropecuarias, Universidad Autonoma de Yucatan, Carretera Merida-Xmatkuil Km. 15.5, Merida 97315, MexicoFacultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, Av. Universidad s/n, Cd. Universitaria, San Nicolas de los Garza 66451, MexicoThe rapid global expansion of <i>Aedes aegypti</i>-borne diseases such as dengue, chikungunya, and Zika has positioned this mosquito as a key target for vector control programs. These programs rely heavily on insecticide use, leading to the widespread emergence of insecticide resistance. Understanding the molecular basis of resistance is essential for developing effective management strategies. In this study, we employed a whole-transcriptome (RNA-seq) approach to analyze gene expression in three <i>Ae. aegypti</i> populations from Mexico that underwent four generations of laboratory selection with deltamethrin. Several cytochrome P450 genes (CYP6AG4, CYP6M5, CYP307A1) and a chitin-binding peritrophin-like gene (Ae-Aper50) were significantly overexpressed following selection, supporting roles for both detoxification and midgut protection. We also observed a consistent downregulation of cuticular protein genes in deltamethrin-selected groups relative to the baseline populations, suggesting their involvement in baseline tolerance rather than induced resistance. Additionally, the overexpression of immune- and stress-related genes, including the RNA helicase MOV-10, indicates that insecticide selection may trigger broader physiological responses. These findings highlight complex, multi-pathway transcriptomic changes associated with resistance development in <i>Ae. aegypti</i>.https://www.mdpi.com/2414-6366/10/6/171<i>Aedes aegypti</i>deltamethrin selectionRNA-seqcytochrome P450peritrophic matrixcuticular proteins |
| spellingShingle | Yamili Contreras-Perera Lucy Mackenzie-Impoinvil Dieunel Derilus Audrey Lenhart Iram P. Rodriguez-Sanchez Pablo Manrique-Saide Adriana E. Flores Deltamethrin Selection Drives Transcriptomic Changes in Detoxification, Immune, and Cuticle Genes in <i>Aedes aegypti</i> Tropical Medicine and Infectious Disease <i>Aedes aegypti</i> deltamethrin selection RNA-seq cytochrome P450 peritrophic matrix cuticular proteins |
| title | Deltamethrin Selection Drives Transcriptomic Changes in Detoxification, Immune, and Cuticle Genes in <i>Aedes aegypti</i> |
| title_full | Deltamethrin Selection Drives Transcriptomic Changes in Detoxification, Immune, and Cuticle Genes in <i>Aedes aegypti</i> |
| title_fullStr | Deltamethrin Selection Drives Transcriptomic Changes in Detoxification, Immune, and Cuticle Genes in <i>Aedes aegypti</i> |
| title_full_unstemmed | Deltamethrin Selection Drives Transcriptomic Changes in Detoxification, Immune, and Cuticle Genes in <i>Aedes aegypti</i> |
| title_short | Deltamethrin Selection Drives Transcriptomic Changes in Detoxification, Immune, and Cuticle Genes in <i>Aedes aegypti</i> |
| title_sort | deltamethrin selection drives transcriptomic changes in detoxification immune and cuticle genes in i aedes aegypti i |
| topic | <i>Aedes aegypti</i> deltamethrin selection RNA-seq cytochrome P450 peritrophic matrix cuticular proteins |
| url | https://www.mdpi.com/2414-6366/10/6/171 |
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