Enhancement of Phenolic and Polyacetylene Accumulation in <i>Lobelia chinensis</i> (Chinese lobelia) Plantlet Cultures Through Yeast Extract and Salicylic Acid Elicitation
<i>Lobelia chinensis</i> (Lour.) is a medicinal plant that contains phytochemicals, such as phenolics and polyacetylene compounds, with beneficial biological activities. In vitro cultures are typically employed for biomass generation and plant multiplication. However, the current biotech...
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2025-05-01
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| author | Xinlei Bai Han-Sol Lee Jong-Eun Han Hosakatte Niranjana Murthy So-Young Park |
| author_facet | Xinlei Bai Han-Sol Lee Jong-Eun Han Hosakatte Niranjana Murthy So-Young Park |
| author_sort | Xinlei Bai |
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| description | <i>Lobelia chinensis</i> (Lour.) is a medicinal plant that contains phytochemicals, such as phenolics and polyacetylene compounds, with beneficial biological activities. In vitro cultures are typically employed for biomass generation and plant multiplication. However, the current biotechnological approaches for producing these chemicals are ineffective, which is why bioelicitors are being used to boost synthesis of these molecules. Plantlet cultures were established in vitro using Murashige and Skoog medium supplemented with 3% sucrose (<i>w</i>/<i>v</i>). Following 4 weeks of culture initiation, the plantlet cultures were treated with 0, 25, 50, 100, or 200 mg L<sup>−1</sup> of yeast extract (YE) or with 0, 25, 50, 100, or 200 µM of salicylic acid (SA) for 1 week to boost the synthesis of bioactive compounds. The amounts of total phenolics, total flavonoids, specific phenolics including catechin, phloretic acid, linarin, and polyacetylenes, including lobetyolinin and lobetylin, were considerably elevated in the plantlet cultures treated with 50 mg L<sup>−1</sup> YE and/or 25 µM SA. The 2,2 Diphenyl 1 picrylhydrazyl (DPPH) radical scavenging assay, 2,2′-azino-bis (3-ethybenzothiazoline-6-sulphonic acid) (ABTS) assay, and ferric reducing antioxidant power (FRAP) assay were performed to assess the antioxidant properties of the plantlets. The elicitor-treated plantlets were found to have higher antioxidant activity. Thus, plantlet biomass produced in vitro can be used as a raw material to produce medicinal and nutraceutical products. |
| format | Article |
| id | doaj-art-c4739ab89ebf47e199db82b2795b4abc |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-05-01 |
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| series | Horticulturae |
| spelling | doaj-art-c4739ab89ebf47e199db82b2795b4abc2025-08-20T03:27:15ZengMDPI AGHorticulturae2311-75242025-05-0111661210.3390/horticulturae11060612Enhancement of Phenolic and Polyacetylene Accumulation in <i>Lobelia chinensis</i> (Chinese lobelia) Plantlet Cultures Through Yeast Extract and Salicylic Acid ElicitationXinlei Bai0Han-Sol Lee1Jong-Eun Han2Hosakatte Niranjana Murthy3So-Young Park4Department of Horticultural Science, Chungbuk National University, Cheongju 28644, Republic of KoreaDepartment of Horticultural Science, Chungbuk National University, Cheongju 28644, Republic of KoreaDepartment of Horticultural Science, Chungbuk National University, Cheongju 28644, Republic of KoreaDepartment of Horticultural Science, Chungbuk National University, Cheongju 28644, Republic of KoreaDepartment of Horticultural Science, Chungbuk National University, Cheongju 28644, Republic of Korea<i>Lobelia chinensis</i> (Lour.) is a medicinal plant that contains phytochemicals, such as phenolics and polyacetylene compounds, with beneficial biological activities. In vitro cultures are typically employed for biomass generation and plant multiplication. However, the current biotechnological approaches for producing these chemicals are ineffective, which is why bioelicitors are being used to boost synthesis of these molecules. Plantlet cultures were established in vitro using Murashige and Skoog medium supplemented with 3% sucrose (<i>w</i>/<i>v</i>). Following 4 weeks of culture initiation, the plantlet cultures were treated with 0, 25, 50, 100, or 200 mg L<sup>−1</sup> of yeast extract (YE) or with 0, 25, 50, 100, or 200 µM of salicylic acid (SA) for 1 week to boost the synthesis of bioactive compounds. The amounts of total phenolics, total flavonoids, specific phenolics including catechin, phloretic acid, linarin, and polyacetylenes, including lobetyolinin and lobetylin, were considerably elevated in the plantlet cultures treated with 50 mg L<sup>−1</sup> YE and/or 25 µM SA. The 2,2 Diphenyl 1 picrylhydrazyl (DPPH) radical scavenging assay, 2,2′-azino-bis (3-ethybenzothiazoline-6-sulphonic acid) (ABTS) assay, and ferric reducing antioxidant power (FRAP) assay were performed to assess the antioxidant properties of the plantlets. The elicitor-treated plantlets were found to have higher antioxidant activity. Thus, plantlet biomass produced in vitro can be used as a raw material to produce medicinal and nutraceutical products.https://www.mdpi.com/2311-7524/11/6/612antioxidant activitybioactive compoundsin vitro culturemedicinal plant |
| spellingShingle | Xinlei Bai Han-Sol Lee Jong-Eun Han Hosakatte Niranjana Murthy So-Young Park Enhancement of Phenolic and Polyacetylene Accumulation in <i>Lobelia chinensis</i> (Chinese lobelia) Plantlet Cultures Through Yeast Extract and Salicylic Acid Elicitation Horticulturae antioxidant activity bioactive compounds in vitro culture medicinal plant |
| title | Enhancement of Phenolic and Polyacetylene Accumulation in <i>Lobelia chinensis</i> (Chinese lobelia) Plantlet Cultures Through Yeast Extract and Salicylic Acid Elicitation |
| title_full | Enhancement of Phenolic and Polyacetylene Accumulation in <i>Lobelia chinensis</i> (Chinese lobelia) Plantlet Cultures Through Yeast Extract and Salicylic Acid Elicitation |
| title_fullStr | Enhancement of Phenolic and Polyacetylene Accumulation in <i>Lobelia chinensis</i> (Chinese lobelia) Plantlet Cultures Through Yeast Extract and Salicylic Acid Elicitation |
| title_full_unstemmed | Enhancement of Phenolic and Polyacetylene Accumulation in <i>Lobelia chinensis</i> (Chinese lobelia) Plantlet Cultures Through Yeast Extract and Salicylic Acid Elicitation |
| title_short | Enhancement of Phenolic and Polyacetylene Accumulation in <i>Lobelia chinensis</i> (Chinese lobelia) Plantlet Cultures Through Yeast Extract and Salicylic Acid Elicitation |
| title_sort | enhancement of phenolic and polyacetylene accumulation in i lobelia chinensis i chinese lobelia plantlet cultures through yeast extract and salicylic acid elicitation |
| topic | antioxidant activity bioactive compounds in vitro culture medicinal plant |
| url | https://www.mdpi.com/2311-7524/11/6/612 |
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