Cochlear Organ Dissection, Immunostaining, and Confocal Imaging in Mice
The organ of Corti, located in the inner ear, is the primary organ responsible for animal hearing. Each hair cell has a V-shaped or U-shaped hair bundle composed of actin-filled stereocilia and a kinocilium supported by true transport microtubules. Damage to these structures due to noise exposure, d...
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Bio-protocol LLC
2025-01-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5167&type=0 |
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| author | Chenyu Chen Binjun Chen Xiaoqing Qian Haojie Sun Xiao Fu Dongdong Ren |
| author_facet | Chenyu Chen Binjun Chen Xiaoqing Qian Haojie Sun Xiao Fu Dongdong Ren |
| author_sort | Chenyu Chen |
| collection | DOAJ |
| description | The organ of Corti, located in the inner ear, is the primary organ responsible for animal hearing. Each hair cell has a V-shaped or U-shaped hair bundle composed of actin-filled stereocilia and a kinocilium supported by true transport microtubules. Damage to these structures due to noise exposure, drug toxicity, aging, or environmental factors can lead to hearing loss and other disorders. The challenge when examining auditory organs is their location within the bony labyrinth and their small and fragile nature. This protocol describes the dissection procedure for the cochlear organ, followed by confocal imaging of immunostained endogenous and fluorescent proteins. This approach can be used to understand hair cell physiology and the molecular mechanisms required for normal hearing. |
| format | Article |
| id | doaj-art-c46720c4c516411996e3b836829c6349 |
| institution | Kabale University |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-c46720c4c516411996e3b836829c63492025-02-07T08:16:38ZengBio-protocol LLCBio-Protocol2331-83252025-01-0115210.21769/BioProtoc.5167Cochlear Organ Dissection, Immunostaining, and Confocal Imaging in MiceChenyu Chen0Binjun Chen1Xiaoqing Qian2Haojie Sun3Xiao Fu4Dongdong Ren5ENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Hearing Medicine Research, Eye & ENT Hospital, Fudan University, Shanghai, ChinaENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Hearing Medicine Research, Eye & ENT Hospital, Fudan University, Shanghai, ChinaDepartment of Otorhinolaryngology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Hearing Medicine Research, Eye & ENT Hospital, Fudan University, Shanghai, ChinaENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Hearing Medicine Research, Eye & ENT Hospital, Fudan University, Shanghai, ChinaENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, ChinaNHC Key Laboratory of Hearing Medicine Research, Eye & ENT Hospital, Fudan University, Shanghai, ChinaThe organ of Corti, located in the inner ear, is the primary organ responsible for animal hearing. Each hair cell has a V-shaped or U-shaped hair bundle composed of actin-filled stereocilia and a kinocilium supported by true transport microtubules. Damage to these structures due to noise exposure, drug toxicity, aging, or environmental factors can lead to hearing loss and other disorders. The challenge when examining auditory organs is their location within the bony labyrinth and their small and fragile nature. This protocol describes the dissection procedure for the cochlear organ, followed by confocal imaging of immunostained endogenous and fluorescent proteins. This approach can be used to understand hair cell physiology and the molecular mechanisms required for normal hearing.https://bio-protocol.org/en/bpdetail?id=5167&type=0 |
| spellingShingle | Chenyu Chen Binjun Chen Xiaoqing Qian Haojie Sun Xiao Fu Dongdong Ren Cochlear Organ Dissection, Immunostaining, and Confocal Imaging in Mice Bio-Protocol |
| title | Cochlear Organ Dissection, Immunostaining, and Confocal Imaging in Mice |
| title_full | Cochlear Organ Dissection, Immunostaining, and Confocal Imaging in Mice |
| title_fullStr | Cochlear Organ Dissection, Immunostaining, and Confocal Imaging in Mice |
| title_full_unstemmed | Cochlear Organ Dissection, Immunostaining, and Confocal Imaging in Mice |
| title_short | Cochlear Organ Dissection, Immunostaining, and Confocal Imaging in Mice |
| title_sort | cochlear organ dissection immunostaining and confocal imaging in mice |
| url | https://bio-protocol.org/en/bpdetail?id=5167&type=0 |
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