Optimization of fermentation conditions for 2,3-butanediol production from Helianthus tuberosus hydrolysate by Raoultella ornithinolytica
2,3-butanediol was produced by Raoultella ornithinolytica TH-21 using Helianthus tuberosus hydrolysate as raw material. Fermentation temperature, time, rotation rate and yeast extract mass concentration were selected as the influencing factors, the effects of each single factor on the 2,3-butanediol...
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Editorial Department of China Brewing
2025-03-01
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| Series: | Zhongguo niangzao |
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| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-3-236.pdf |
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| author | LI Feng, YANG Lin, XIE Guoyou, XU Ping |
| author_facet | LI Feng, YANG Lin, XIE Guoyou, XU Ping |
| author_sort | LI Feng, YANG Lin, XIE Guoyou, XU Ping |
| collection | DOAJ |
| description | 2,3-butanediol was produced by Raoultella ornithinolytica TH-21 using Helianthus tuberosus hydrolysate as raw material. Fermentation temperature, time, rotation rate and yeast extract mass concentration were selected as the influencing factors, the effects of each single factor on the 2,3-butanediol production were investigated, and the fermentation conditions were optimized by response surface tests. The results showed that the optimal fermentation conditions were as follows: fermentation temperature 28 ℃, fermentation time 25 h, rotation rate 270 r/min and yeast extract mass concentration 12 g/L. Under these optimal conditions, the 2,3-butanediol production could reach 19.2 g/L, which was increased by 22.68% compared to that before optimization. When the fermentation time was 25 h, the mass concentration of reducing sugar was 3.75 g/L, and the mass concentration of 2,3-butanediol was the highest (19.2 g/L), indicating that the R. ornithinolytica TH-21 metabolized the carbohydrates to produce 2,3-butanediol within the initial 25 h. |
| format | Article |
| id | doaj-art-c46586f079dc446cb43c1b44643ea9a4 |
| institution | OA Journals |
| issn | 0254-5071 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Editorial Department of China Brewing |
| record_format | Article |
| series | Zhongguo niangzao |
| spelling | doaj-art-c46586f079dc446cb43c1b44643ea9a42025-08-20T01:47:54ZengEditorial Department of China BrewingZhongguo niangzao0254-50712025-03-0144323624110.11882/j.issn.0254-5071.2025.03.035Optimization of fermentation conditions for 2,3-butanediol production from Helianthus tuberosus hydrolysate by Raoultella ornithinolyticaLI Feng, YANG Lin, XIE Guoyou, XU Ping0School of Chemistry and Chemical Engineering, Qiannan Normal College for Nationalities, Duyun 558000, China2,3-butanediol was produced by Raoultella ornithinolytica TH-21 using Helianthus tuberosus hydrolysate as raw material. Fermentation temperature, time, rotation rate and yeast extract mass concentration were selected as the influencing factors, the effects of each single factor on the 2,3-butanediol production were investigated, and the fermentation conditions were optimized by response surface tests. The results showed that the optimal fermentation conditions were as follows: fermentation temperature 28 ℃, fermentation time 25 h, rotation rate 270 r/min and yeast extract mass concentration 12 g/L. Under these optimal conditions, the 2,3-butanediol production could reach 19.2 g/L, which was increased by 22.68% compared to that before optimization. When the fermentation time was 25 h, the mass concentration of reducing sugar was 3.75 g/L, and the mass concentration of 2,3-butanediol was the highest (19.2 g/L), indicating that the R. ornithinolytica TH-21 metabolized the carbohydrates to produce 2,3-butanediol within the initial 25 h.https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-3-236.pdf2,3-butanediol|helianthus tuberosus| raoultella ornithinolytica|fermentation condition optimization|response surface methodology |
| spellingShingle | LI Feng, YANG Lin, XIE Guoyou, XU Ping Optimization of fermentation conditions for 2,3-butanediol production from Helianthus tuberosus hydrolysate by Raoultella ornithinolytica Zhongguo niangzao 2,3-butanediol|helianthus tuberosus| raoultella ornithinolytica|fermentation condition optimization|response surface methodology |
| title | Optimization of fermentation conditions for 2,3-butanediol production from Helianthus tuberosus hydrolysate by Raoultella ornithinolytica |
| title_full | Optimization of fermentation conditions for 2,3-butanediol production from Helianthus tuberosus hydrolysate by Raoultella ornithinolytica |
| title_fullStr | Optimization of fermentation conditions for 2,3-butanediol production from Helianthus tuberosus hydrolysate by Raoultella ornithinolytica |
| title_full_unstemmed | Optimization of fermentation conditions for 2,3-butanediol production from Helianthus tuberosus hydrolysate by Raoultella ornithinolytica |
| title_short | Optimization of fermentation conditions for 2,3-butanediol production from Helianthus tuberosus hydrolysate by Raoultella ornithinolytica |
| title_sort | optimization of fermentation conditions for 2 3 butanediol production from helianthus tuberosus hydrolysate by raoultella ornithinolytica |
| topic | 2,3-butanediol|helianthus tuberosus| raoultella ornithinolytica|fermentation condition optimization|response surface methodology |
| url | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-3-236.pdf |
| work_keys_str_mv | AT lifengyanglinxieguoyouxuping optimizationoffermentationconditionsfor23butanediolproductionfromhelianthustuberosushydrolysatebyraoultellaornithinolytica |