Multi-locus sequence typing of Escherichia coli isolated from clinical samples in Jordan
Introduction: Escherichia coli (E. coli) is the major cause of extraintestinal infections in the urinary tracts and bloodstream in humans in the community and health care institutions. Several studies on the genetic characterization of E. coli among clinical and environmental isolates were performe...
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The Journal of Infection in Developing Countries
2024-04-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/18810 |
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| author | AbdelRahman M Zueter Taghrid Mharib Dalal Shqair Mohammad Al-Tamimi Hana M Sawan Amani Zaiter Hadeel Albalawi Dua’a Al Balawi |
| author_facet | AbdelRahman M Zueter Taghrid Mharib Dalal Shqair Mohammad Al-Tamimi Hana M Sawan Amani Zaiter Hadeel Albalawi Dua’a Al Balawi |
| author_sort | AbdelRahman M Zueter |
| collection | DOAJ |
| description |
Introduction: Escherichia coli (E. coli) is the major cause of extraintestinal infections in the urinary tracts and bloodstream in humans in the community and health care institutions. Several studies on the genetic characterization of E. coli among clinical and environmental isolates were performed and revealed a wide diversity of sequence types (STs). In Jordan, phenotypic and genetic features of E. coli were extensively studied but there is still a need to identify the STs that inhabit the community.
Methodology: In this study, multi-locus sequence typing (MLST) was performed on archived clinical E. coli isolates collected from different hospitals in Jordan and the identified STs were extensively analyzed.
Results: Genotyping of 92 E. coli isolates revealed 34 STs and 9 clonal complexes. The frequencies of STs ranged between 1 to 23 observations. The most frequent STs among E. coli isolates were ST131 (n = 23), ST69 (n = 19), ST998 (n = 7), ST2083 (n = 5), and ST540 (n = 4). These five ST accounted for up to 60% of the 92 E. coli isolates. Based on the MLST database, the STs reported in this work were world widely recognized in humans, animals, and in the environment.
Conclusions: This study has elaborated more knowledge about the genotypes of E. coli in Jordan, with recommendations for future studies to correlate its genotypes with virulence and resistance genes.
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| format | Article |
| id | doaj-art-c45526dfd549458387b80fa8297c3cdc |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-c45526dfd549458387b80fa8297c3cdc2025-08-20T02:16:06ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802024-04-01180410.3855/jidc.18810Multi-locus sequence typing of Escherichia coli isolated from clinical samples in JordanAbdelRahman M Zueter0Taghrid Mharib1Dalal Shqair2Mohammad Al-Tamimi3Hana M Sawan4Amani Zaiter5Hadeel Albalawi6Dua’a Al Balawi7Department of Medical Laboratory Sciences, Faculty of Applied Health Sciences, The Hashemite University, P.O box 330127, Zarqa 13133, JordanDepartment of Medical Laboratory Sciences, Faculty of Applied Health Sciences, The Hashemite University, P.O box 330127, Zarqa 13133, JordanDepartment of Basic Medical Sciences, Faculty of Applied Health Sciences, The Hashemite University, P.O box 330127, Zarqa 13133, JordanDepartment of Basic Medical Sciences, Faculty of Medicine, The Hashemite University, P.O box 330127, Zarqa 13133, JordanFaculty of Pharmacy, Zarqa University, Zarqa, JordanDepartment of Science, Omareyah School, Amman, JordanDepartment of Basic Medical Sciences, Faculty of Medicine, The Hashemite University, P.O box 330127, Zarqa 13133, JordanDepartment of Basic Medical Sciences, Faculty of Medicine, The Hashemite University, P.O box 330127, Zarqa 13133, Jordan Introduction: Escherichia coli (E. coli) is the major cause of extraintestinal infections in the urinary tracts and bloodstream in humans in the community and health care institutions. Several studies on the genetic characterization of E. coli among clinical and environmental isolates were performed and revealed a wide diversity of sequence types (STs). In Jordan, phenotypic and genetic features of E. coli were extensively studied but there is still a need to identify the STs that inhabit the community. Methodology: In this study, multi-locus sequence typing (MLST) was performed on archived clinical E. coli isolates collected from different hospitals in Jordan and the identified STs were extensively analyzed. Results: Genotyping of 92 E. coli isolates revealed 34 STs and 9 clonal complexes. The frequencies of STs ranged between 1 to 23 observations. The most frequent STs among E. coli isolates were ST131 (n = 23), ST69 (n = 19), ST998 (n = 7), ST2083 (n = 5), and ST540 (n = 4). These five ST accounted for up to 60% of the 92 E. coli isolates. Based on the MLST database, the STs reported in this work were world widely recognized in humans, animals, and in the environment. Conclusions: This study has elaborated more knowledge about the genotypes of E. coli in Jordan, with recommendations for future studies to correlate its genotypes with virulence and resistance genes. https://jidc.org/index.php/journal/article/view/18810Escherichia coliJordanUTIMLSTSequence type |
| spellingShingle | AbdelRahman M Zueter Taghrid Mharib Dalal Shqair Mohammad Al-Tamimi Hana M Sawan Amani Zaiter Hadeel Albalawi Dua’a Al Balawi Multi-locus sequence typing of Escherichia coli isolated from clinical samples in Jordan Journal of Infection in Developing Countries Escherichia coli Jordan UTI MLST Sequence type |
| title | Multi-locus sequence typing of Escherichia coli isolated from clinical samples in Jordan |
| title_full | Multi-locus sequence typing of Escherichia coli isolated from clinical samples in Jordan |
| title_fullStr | Multi-locus sequence typing of Escherichia coli isolated from clinical samples in Jordan |
| title_full_unstemmed | Multi-locus sequence typing of Escherichia coli isolated from clinical samples in Jordan |
| title_short | Multi-locus sequence typing of Escherichia coli isolated from clinical samples in Jordan |
| title_sort | multi locus sequence typing of escherichia coli isolated from clinical samples in jordan |
| topic | Escherichia coli Jordan UTI MLST Sequence type |
| url | https://jidc.org/index.php/journal/article/view/18810 |
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