Methodology-Dependent Reversals in Root Decomposition: Divergent Regulation by Forest Gap and Root Order in <i>Pinus massoniana</i>

Understanding root decomposition dynamics is essential to address declining carbon sequestration and nutrient imbalances in monoculture plantations. This study elucidates how forest gaps regulate <i>Pinus massoniana</i> root decomposition through comparative methodological analysis, prov...

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Main Authors: Haifeng Yin, Jie Zeng, Size Liu, Yu Su, Anwei Yu, Xianwei Li
Format: Article
Language:English
Published: MDPI AG 2025-08-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/15/2365
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author Haifeng Yin
Jie Zeng
Size Liu
Yu Su
Anwei Yu
Xianwei Li
author_facet Haifeng Yin
Jie Zeng
Size Liu
Yu Su
Anwei Yu
Xianwei Li
author_sort Haifeng Yin
collection DOAJ
description Understanding root decomposition dynamics is essential to address declining carbon sequestration and nutrient imbalances in monoculture plantations. This study elucidates how forest gaps regulate <i>Pinus massoniana</i> root decomposition through comparative methodological analysis, providing theoretical foundations for near-natural forest management and carbon–nitrogen cycle optimization in plantations. The results showed the following: (1) Root decomposition was significantly accelerated by the in situ soil litterbag method (ISLM) versus the traditional litterbag method (LM) (decomposition rate (<i>k</i>) = 0.459 vs. 0.188), reducing the 95% decomposition time (<i>T<sub>0.95</sub></i>) by nearly nine years (6.53 years vs. 15.95 years). ISLM concurrently elevated the root potassium concentration and reconfigured the relationships between root decomposition and soil nutrients. (2) Lower-order roots (orders 1–3) decomposed significantly faster than higher-order roots (orders 4–5) (<i>k</i> = 0.455 vs. 0.193). This disparity was amplified under ISLM (lower-/higher-order root <i>k</i> ratio = 4.1) but diminished or reversed under LM (lower-/higher-order root <i>k</i> ratio = 0.8). (3) Forest gaps regulated decomposition through temporal phase interactions, accelerating decomposition initially (0–360 days) while inhibiting it later (360–720 days), particularly for higher-order roots. Notably, forest gap effects fundamentally reversed between methodologies (slight promotion under LM vs. significant inhibition under ISLM). Our study reveals that conventional LM may obscure genuine ecological interactions during root decomposition, confirms lower-order roots as rapid nutrient-cycling pathways, provides crucial methodological corrections for plantation nutrient models, and advances theoretical foundations for precision management of <i>P. massoniana</i> plantations.
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institution Kabale University
issn 2223-7747
language English
publishDate 2025-08-01
publisher MDPI AG
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series Plants
spelling doaj-art-c453eee9354b4e4e982c76c85a8de8cb2025-08-20T03:36:22ZengMDPI AGPlants2223-77472025-08-011415236510.3390/plants14152365Methodology-Dependent Reversals in Root Decomposition: Divergent Regulation by Forest Gap and Root Order in <i>Pinus massoniana</i>Haifeng Yin0Jie Zeng1Size Liu2Yu Su3Anwei Yu4Xianwei Li5Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, ChinaResearch Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, ChinaSichuan Academy of Forestry, Chengdu 610081, ChinaGuangzhou Institute of Forestry and Landscape Architecture, Guangzhou 510405, ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu 611130, ChinaUnderstanding root decomposition dynamics is essential to address declining carbon sequestration and nutrient imbalances in monoculture plantations. This study elucidates how forest gaps regulate <i>Pinus massoniana</i> root decomposition through comparative methodological analysis, providing theoretical foundations for near-natural forest management and carbon–nitrogen cycle optimization in plantations. The results showed the following: (1) Root decomposition was significantly accelerated by the in situ soil litterbag method (ISLM) versus the traditional litterbag method (LM) (decomposition rate (<i>k</i>) = 0.459 vs. 0.188), reducing the 95% decomposition time (<i>T<sub>0.95</sub></i>) by nearly nine years (6.53 years vs. 15.95 years). ISLM concurrently elevated the root potassium concentration and reconfigured the relationships between root decomposition and soil nutrients. (2) Lower-order roots (orders 1–3) decomposed significantly faster than higher-order roots (orders 4–5) (<i>k</i> = 0.455 vs. 0.193). This disparity was amplified under ISLM (lower-/higher-order root <i>k</i> ratio = 4.1) but diminished or reversed under LM (lower-/higher-order root <i>k</i> ratio = 0.8). (3) Forest gaps regulated decomposition through temporal phase interactions, accelerating decomposition initially (0–360 days) while inhibiting it later (360–720 days), particularly for higher-order roots. Notably, forest gap effects fundamentally reversed between methodologies (slight promotion under LM vs. significant inhibition under ISLM). Our study reveals that conventional LM may obscure genuine ecological interactions during root decomposition, confirms lower-order roots as rapid nutrient-cycling pathways, provides crucial methodological corrections for plantation nutrient models, and advances theoretical foundations for precision management of <i>P. massoniana</i> plantations.https://www.mdpi.com/2223-7747/14/15/2365root decompositionin situ soil litterbag methodforest gapfine root orderMasson pinenutrient cycling
spellingShingle Haifeng Yin
Jie Zeng
Size Liu
Yu Su
Anwei Yu
Xianwei Li
Methodology-Dependent Reversals in Root Decomposition: Divergent Regulation by Forest Gap and Root Order in <i>Pinus massoniana</i>
Plants
root decomposition
in situ soil litterbag method
forest gap
fine root order
Masson pine
nutrient cycling
title Methodology-Dependent Reversals in Root Decomposition: Divergent Regulation by Forest Gap and Root Order in <i>Pinus massoniana</i>
title_full Methodology-Dependent Reversals in Root Decomposition: Divergent Regulation by Forest Gap and Root Order in <i>Pinus massoniana</i>
title_fullStr Methodology-Dependent Reversals in Root Decomposition: Divergent Regulation by Forest Gap and Root Order in <i>Pinus massoniana</i>
title_full_unstemmed Methodology-Dependent Reversals in Root Decomposition: Divergent Regulation by Forest Gap and Root Order in <i>Pinus massoniana</i>
title_short Methodology-Dependent Reversals in Root Decomposition: Divergent Regulation by Forest Gap and Root Order in <i>Pinus massoniana</i>
title_sort methodology dependent reversals in root decomposition divergent regulation by forest gap and root order in i pinus massoniana i
topic root decomposition
in situ soil litterbag method
forest gap
fine root order
Masson pine
nutrient cycling
url https://www.mdpi.com/2223-7747/14/15/2365
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