Polysaccharide-based aerogel dressing integrated with MOF-loaded quercetin with multifunctional effects for promoting infected wound healing

Infectious wounds disrupt the progression through the stages of wound healing, resulting in the development of chronic wounds. However, the current therapeutic approach is limited and requires repeated administrations. A major challenge in infectious wounds is to achieve sustained drug release. Here...

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Main Authors: Xiaomin Wu, Qisong Liu, Alena Rasiuk, Shkuryn Siarhei Vladimirovich, Yu Pan, Bixia Lin, Ying Liu, Wenqiang Li, Genghao Li, Wensong Li, Cuiping Zhang, Xiaohua Pan
Format: Article
Language:English
Published: AIP Publishing LLC 2025-04-01
Series:APL Materials
Online Access:http://dx.doi.org/10.1063/5.0258842
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author Xiaomin Wu
Qisong Liu
Alena Rasiuk
Shkuryn Siarhei Vladimirovich
Yu Pan
Bixia Lin
Ying Liu
Wenqiang Li
Genghao Li
Wensong Li
Cuiping Zhang
Xiaohua Pan
author_facet Xiaomin Wu
Qisong Liu
Alena Rasiuk
Shkuryn Siarhei Vladimirovich
Yu Pan
Bixia Lin
Ying Liu
Wenqiang Li
Genghao Li
Wensong Li
Cuiping Zhang
Xiaohua Pan
author_sort Xiaomin Wu
collection DOAJ
description Infectious wounds disrupt the progression through the stages of wound healing, resulting in the development of chronic wounds. However, the current therapeutic approach is limited and requires repeated administrations. A major challenge in infectious wounds is to achieve sustained drug release. Herein, quercetin with anti-inflammatory and antibacterial properties was encapsulated within amino-functionalized UIO-66 metal-organic framework (MOF) to form MOF@Que NPs. MOF@Que NPs were then loaded into an aerogel constructed with agarose, aldehyde hyaluronic acid, and carboxymethyl chitosan to obtain ACA-MOF@Que for targeting different phases of wound healing. The polysaccharide-based aerogel was formed by the entanglement of polymer chains and the Schiff base reaction, which exhibited uniform pore structures and low-swelling properties. Furthermore, the ACA-MOF@Que aerogel also exhibited antioxidant, anti-inflammatory, and antibacterial properties. In vivo and in vitro experiments confirmed that the ACA-MOF@Que aerogel could promote hemostasis in the initial stage of wound healing, reduce cellular inflammatory infiltration, and inhibit the inflammatory response by regulating macrophage polarization during the inflammatory stage of wound healing. Finally, the ACA-MOF@Que aerogel could promote angiogenesis and increase collagen deposition in the later stage of wound healing. Compared with the untreated group, the wounds of rats treated with the ACA-MOF@Que aerogel had almost completely healed on the 14th day. Therefore, the ACA-MOF@Que aerogel plays multiple roles at various stages of wound healing, providing a promising therapeutic approach for the clinical application of chronic wounds.
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spelling doaj-art-c45059e6d2c64bf3b3de53419bd43c782025-08-20T02:11:08ZengAIP Publishing LLCAPL Materials2166-532X2025-04-01134041113041113-1310.1063/5.0258842Polysaccharide-based aerogel dressing integrated with MOF-loaded quercetin with multifunctional effects for promoting infected wound healingXiaomin Wu0Qisong Liu1Alena Rasiuk2Shkuryn Siarhei Vladimirovich3Yu Pan4Bixia Lin5Ying Liu6Wenqiang Li7Genghao Li8Wensong Li9Cuiping Zhang10Xiaohua Pan11Institute of Clinical Translation and Regenerative Medicine, The Second Affiliated Hospital of Shenzhen University, Shenzhen, ChinaInstitute of Clinical Translation and Regenerative Medicine, The Second Affiliated Hospital of Shenzhen University, Shenzhen, ChinaRepublican Scientific and Practical Center of Transfusiology and Medical Biotechnology, The Ministry of Health of the Republic of Belarus, Minsk, BelarusMinsk Regional Clinical Hospital, Minsk, BelarusInstitute of Clinical Translation and Regenerative Medicine, The Second Affiliated Hospital of Shenzhen University, Shenzhen, ChinaGuangdong Huayi Biomedical Science and Technology Center, Guangzhou, ChinaGuangdong Huayi Biomedical Science and Technology Center, Guangzhou, ChinaEngineering Technology Research Center for Sports Assistive Devices of Guangdong, Guangzhou Sport University, Guangzhou, ChinaInstitute of Clinical Translation and Regenerative Medicine, The Second Affiliated Hospital of Shenzhen University, Shenzhen, ChinaWard for Trauma and Burn Care, The Second Affiliated Hospital of Shenzhen University, Shenzhen, ChinaResearch Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Department, PLA General Hospital, Beijing, ChinaInstitute of Clinical Translation and Regenerative Medicine, The Second Affiliated Hospital of Shenzhen University, Shenzhen, ChinaInfectious wounds disrupt the progression through the stages of wound healing, resulting in the development of chronic wounds. However, the current therapeutic approach is limited and requires repeated administrations. A major challenge in infectious wounds is to achieve sustained drug release. Herein, quercetin with anti-inflammatory and antibacterial properties was encapsulated within amino-functionalized UIO-66 metal-organic framework (MOF) to form MOF@Que NPs. MOF@Que NPs were then loaded into an aerogel constructed with agarose, aldehyde hyaluronic acid, and carboxymethyl chitosan to obtain ACA-MOF@Que for targeting different phases of wound healing. The polysaccharide-based aerogel was formed by the entanglement of polymer chains and the Schiff base reaction, which exhibited uniform pore structures and low-swelling properties. Furthermore, the ACA-MOF@Que aerogel also exhibited antioxidant, anti-inflammatory, and antibacterial properties. In vivo and in vitro experiments confirmed that the ACA-MOF@Que aerogel could promote hemostasis in the initial stage of wound healing, reduce cellular inflammatory infiltration, and inhibit the inflammatory response by regulating macrophage polarization during the inflammatory stage of wound healing. Finally, the ACA-MOF@Que aerogel could promote angiogenesis and increase collagen deposition in the later stage of wound healing. Compared with the untreated group, the wounds of rats treated with the ACA-MOF@Que aerogel had almost completely healed on the 14th day. Therefore, the ACA-MOF@Que aerogel plays multiple roles at various stages of wound healing, providing a promising therapeutic approach for the clinical application of chronic wounds.http://dx.doi.org/10.1063/5.0258842
spellingShingle Xiaomin Wu
Qisong Liu
Alena Rasiuk
Shkuryn Siarhei Vladimirovich
Yu Pan
Bixia Lin
Ying Liu
Wenqiang Li
Genghao Li
Wensong Li
Cuiping Zhang
Xiaohua Pan
Polysaccharide-based aerogel dressing integrated with MOF-loaded quercetin with multifunctional effects for promoting infected wound healing
APL Materials
title Polysaccharide-based aerogel dressing integrated with MOF-loaded quercetin with multifunctional effects for promoting infected wound healing
title_full Polysaccharide-based aerogel dressing integrated with MOF-loaded quercetin with multifunctional effects for promoting infected wound healing
title_fullStr Polysaccharide-based aerogel dressing integrated with MOF-loaded quercetin with multifunctional effects for promoting infected wound healing
title_full_unstemmed Polysaccharide-based aerogel dressing integrated with MOF-loaded quercetin with multifunctional effects for promoting infected wound healing
title_short Polysaccharide-based aerogel dressing integrated with MOF-loaded quercetin with multifunctional effects for promoting infected wound healing
title_sort polysaccharide based aerogel dressing integrated with mof loaded quercetin with multifunctional effects for promoting infected wound healing
url http://dx.doi.org/10.1063/5.0258842
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