Indirect Spectrophotometric Determination of Antidepressant Drugs by Oxidation Using Ponceau 4R Dye
A simple and sensitive indirect spectrophotometric method has been developed for the assay of Adrenaline, Dopamine hydrochloride, and Methyldopa in their pure and pharmaceutical preparations. The method is based on the principle of oxidation of the above drugs with a known amount of the oxidizing ag...
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National Centre of Excellence in Analytical Chemsitry
2025-06-01
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| Series: | Pakistan Journal of Analytical & Environmental Chemistry |
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| Online Access: | https://pjaec.pk/index.php/pjaec/article/view/11 |
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| author | Asmaa H. Abbas Subhi M. J. Al-Mtwaiti Theia’a N. Al-Sabha |
| author_facet | Asmaa H. Abbas Subhi M. J. Al-Mtwaiti Theia’a N. Al-Sabha |
| author_sort | Asmaa H. Abbas |
| collection | DOAJ |
| description | A simple and sensitive indirect spectrophotometric method has been developed for the assay of Adrenaline, Dopamine hydrochloride, and Methyldopa in their pure and pharmaceutical preparations. The method is based on the principle of oxidation of the above drugs with a known amount of the oxidizing agent, N-bromosuccinimide (NBS), in hydrochloric acid. The unreacted oxidant bleaches Ponceau 4R dye, then the absorbance of the remaining dye (ΔA) is measured at 507 nm. The method obeyed Beer’s law within the concentration range of 3.5-10, 4-15, and 5-20 μg/mL, with molar absorptivity values of 8.01×104, 5.32×104, and 4.93×104 L. mol-1cm-1 for Adrenaline, Dopamine hydrochloride and Methyldopa, respectively. The recovery % ranged between 99.15% and 100.61 %, and the relative standard deviation (RSD) was less than 1.42 % for all drug compounds. The method was applied successfully to pharmaceutical preparations for the studied medicinal compounds from different origins, as its results were in good agreement with the original content of the drugs in their pharmaceutical formulations and with the standard addition procedure, which proved that there was no interference by the excipients |
| format | Article |
| id | doaj-art-c4486bd996a14e0aae71e84837d19161 |
| institution | OA Journals |
| issn | 1996-918X 2221-5255 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | National Centre of Excellence in Analytical Chemsitry |
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| series | Pakistan Journal of Analytical & Environmental Chemistry |
| spelling | doaj-art-c4486bd996a14e0aae71e84837d191612025-08-20T02:07:59ZengNational Centre of Excellence in Analytical ChemsitryPakistan Journal of Analytical & Environmental Chemistry1996-918X2221-52552025-06-01250111312510.21743/pjaec/2024.06.10Indirect Spectrophotometric Determination of Antidepressant Drugs by Oxidation Using Ponceau 4R DyeAsmaa H. Abbas0Subhi M. J. Al-Mtwaiti 1Theia’a N. Al-Sabha2Department of Chemistry, College of Education for Pure Sciences, University of Mosul, Mosul, IraqDepartment of Chemistry, College of Education for Pure Sciences, University of Mosul, Mosul, IraqDepartment of Chemistry, College of Education for Pure Sciences, University of Mosul, Mosul, IraqA simple and sensitive indirect spectrophotometric method has been developed for the assay of Adrenaline, Dopamine hydrochloride, and Methyldopa in their pure and pharmaceutical preparations. The method is based on the principle of oxidation of the above drugs with a known amount of the oxidizing agent, N-bromosuccinimide (NBS), in hydrochloric acid. The unreacted oxidant bleaches Ponceau 4R dye, then the absorbance of the remaining dye (ΔA) is measured at 507 nm. The method obeyed Beer’s law within the concentration range of 3.5-10, 4-15, and 5-20 μg/mL, with molar absorptivity values of 8.01×104, 5.32×104, and 4.93×104 L. mol-1cm-1 for Adrenaline, Dopamine hydrochloride and Methyldopa, respectively. The recovery % ranged between 99.15% and 100.61 %, and the relative standard deviation (RSD) was less than 1.42 % for all drug compounds. The method was applied successfully to pharmaceutical preparations for the studied medicinal compounds from different origins, as its results were in good agreement with the original content of the drugs in their pharmaceutical formulations and with the standard addition procedure, which proved that there was no interference by the excipientshttps://pjaec.pk/index.php/pjaec/article/view/11antidepressant drugsponceau 4rnbs |
| spellingShingle | Asmaa H. Abbas Subhi M. J. Al-Mtwaiti Theia’a N. Al-Sabha Indirect Spectrophotometric Determination of Antidepressant Drugs by Oxidation Using Ponceau 4R Dye Pakistan Journal of Analytical & Environmental Chemistry antidepressant drugs ponceau 4r nbs |
| title | Indirect Spectrophotometric Determination of Antidepressant Drugs by Oxidation Using Ponceau 4R Dye |
| title_full | Indirect Spectrophotometric Determination of Antidepressant Drugs by Oxidation Using Ponceau 4R Dye |
| title_fullStr | Indirect Spectrophotometric Determination of Antidepressant Drugs by Oxidation Using Ponceau 4R Dye |
| title_full_unstemmed | Indirect Spectrophotometric Determination of Antidepressant Drugs by Oxidation Using Ponceau 4R Dye |
| title_short | Indirect Spectrophotometric Determination of Antidepressant Drugs by Oxidation Using Ponceau 4R Dye |
| title_sort | indirect spectrophotometric determination of antidepressant drugs by oxidation using ponceau 4r dye |
| topic | antidepressant drugs ponceau 4r nbs |
| url | https://pjaec.pk/index.php/pjaec/article/view/11 |
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