Screening and optimization of cyclodextrin glucanotransferase production by an alkaliphile Paenibacillus daejeonensis P-83
Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) is the member of starch degrading enzymes that catalyses an intramolecular transglycosylation reaction to produce cyclodextrins. CGTase producing 141 isolates were obtained from different agricultural fields (paddy, corn, potato, sorghum, and mil...
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Elsevier
2025-06-01
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| Series: | The Microbe |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950194625001268 |
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| author | Dimple S. Pardhi Khushbu Rabadiya Rushikesh G. Joshi Vikram H. Raval Rakeshkumar R. Panchal Kiransinh N. Rajput |
| author_facet | Dimple S. Pardhi Khushbu Rabadiya Rushikesh G. Joshi Vikram H. Raval Rakeshkumar R. Panchal Kiransinh N. Rajput |
| author_sort | Dimple S. Pardhi |
| collection | DOAJ |
| description | Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) is the member of starch degrading enzymes that catalyses an intramolecular transglycosylation reaction to produce cyclodextrins. CGTase producing 141 isolates were obtained from different agricultural fields (paddy, corn, potato, sorghum, and millet), gardens, and industrial waste soil samples. Amongst these 141, an alkaliphilic CGTase producing bacterial isolate Paenibacillus daejeonensis P-83 was selected. Although the CGTase showed the ability to produce α-, β-, and γ-cyclodextrins, the optimization studies were focused on β-CD production. One-factor-at-a-time (OFAT) increased the initial CGTase production from 1.05 U/ml to 2.76 U/ml with optimal conditions like, 3 % (w/v) water chestnut flour, 0.5 % (w/v) peptone, 0.5 % (w/v) yeast extract, 0.02 % (w/v) MgSO4, 0.1 % (w/v) K2HPO4, pH 11 ± 0.2, 6 % (v/v) inoculum, and incubation at 30 °C ± 0.2 for 72 hours on a rotary shaker (150 rpm). The CGTase production was increased upto 5.23 U/ml by statistical optimization via Central Component Design (CCD) using three variables i.e., water chestnut flour (3.65 %; w/v), peptone (1.1 %; w/v), and yeast extract (0.5 %; w/v). The optimization process significantly increased the CGTase production by 2.55 and 4.9 times using OFAT and CCD, respectively. |
| format | Article |
| id | doaj-art-c447e704bf3b4f32a6ae9d8b50bfb80d |
| institution | OA Journals |
| issn | 2950-1946 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | The Microbe |
| spelling | doaj-art-c447e704bf3b4f32a6ae9d8b50bfb80d2025-08-20T02:19:47ZengElsevierThe Microbe2950-19462025-06-01710035810.1016/j.microb.2025.100358Screening and optimization of cyclodextrin glucanotransferase production by an alkaliphile Paenibacillus daejeonensis P-83Dimple S. Pardhi0Khushbu Rabadiya1Rushikesh G. Joshi2Vikram H. Raval3Rakeshkumar R. Panchal4Kiransinh N. Rajput5Department of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad, Gujarat 380009, IndiaDepartment of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad, Gujarat 380009, IndiaDepartment of Biochemistry and Forensic Science, School of Sciences, Gujarat University, Ahmedabad, Gujarat 380009, IndiaDepartment of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad, Gujarat 380009, IndiaDepartment of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad, Gujarat 380009, IndiaDepartment of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad, Gujarat 380009, India; Corresponding author.Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) is the member of starch degrading enzymes that catalyses an intramolecular transglycosylation reaction to produce cyclodextrins. CGTase producing 141 isolates were obtained from different agricultural fields (paddy, corn, potato, sorghum, and millet), gardens, and industrial waste soil samples. Amongst these 141, an alkaliphilic CGTase producing bacterial isolate Paenibacillus daejeonensis P-83 was selected. Although the CGTase showed the ability to produce α-, β-, and γ-cyclodextrins, the optimization studies were focused on β-CD production. One-factor-at-a-time (OFAT) increased the initial CGTase production from 1.05 U/ml to 2.76 U/ml with optimal conditions like, 3 % (w/v) water chestnut flour, 0.5 % (w/v) peptone, 0.5 % (w/v) yeast extract, 0.02 % (w/v) MgSO4, 0.1 % (w/v) K2HPO4, pH 11 ± 0.2, 6 % (v/v) inoculum, and incubation at 30 °C ± 0.2 for 72 hours on a rotary shaker (150 rpm). The CGTase production was increased upto 5.23 U/ml by statistical optimization via Central Component Design (CCD) using three variables i.e., water chestnut flour (3.65 %; w/v), peptone (1.1 %; w/v), and yeast extract (0.5 %; w/v). The optimization process significantly increased the CGTase production by 2.55 and 4.9 times using OFAT and CCD, respectively.http://www.sciencedirect.com/science/article/pii/S2950194625001268CGTaseStarchCyclodextrinPaenibacillus daejeonensis P-83One-factor-at-a-timeShake flask culture |
| spellingShingle | Dimple S. Pardhi Khushbu Rabadiya Rushikesh G. Joshi Vikram H. Raval Rakeshkumar R. Panchal Kiransinh N. Rajput Screening and optimization of cyclodextrin glucanotransferase production by an alkaliphile Paenibacillus daejeonensis P-83 The Microbe CGTase Starch Cyclodextrin Paenibacillus daejeonensis P-83 One-factor-at-a-time Shake flask culture |
| title | Screening and optimization of cyclodextrin glucanotransferase production by an alkaliphile Paenibacillus daejeonensis P-83 |
| title_full | Screening and optimization of cyclodextrin glucanotransferase production by an alkaliphile Paenibacillus daejeonensis P-83 |
| title_fullStr | Screening and optimization of cyclodextrin glucanotransferase production by an alkaliphile Paenibacillus daejeonensis P-83 |
| title_full_unstemmed | Screening and optimization of cyclodextrin glucanotransferase production by an alkaliphile Paenibacillus daejeonensis P-83 |
| title_short | Screening and optimization of cyclodextrin glucanotransferase production by an alkaliphile Paenibacillus daejeonensis P-83 |
| title_sort | screening and optimization of cyclodextrin glucanotransferase production by an alkaliphile paenibacillus daejeonensis p 83 |
| topic | CGTase Starch Cyclodextrin Paenibacillus daejeonensis P-83 One-factor-at-a-time Shake flask culture |
| url | http://www.sciencedirect.com/science/article/pii/S2950194625001268 |
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