Enhancement of Anti-Staling Properties of Rice Bread Through Fermentation Rice Flour with Three Lactic Acid Bacteria

This study investigated the effects of <i>Lactococcus lactis</i> subsp. 1.2472 (L)-, <i>Streptococcus thermophilus</i> 1.2718 (S)-, and thermostable <i>Lactobacillus rhamnosus</i> HCUL 1.1901-1912 (T)-fermented rice flour with inoculum levels of 3–11% (<i>w&...

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Main Authors: Zhiqi Wang, Zhaosen Yuan, Xinlai Dou, Wanshan Yang, Huining Zhang, Yue Zhang, Fenglian Chen, Yanling Hao
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Foods
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Online Access:https://www.mdpi.com/2304-8158/14/15/2674
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author Zhiqi Wang
Zhaosen Yuan
Xinlai Dou
Wanshan Yang
Huining Zhang
Yue Zhang
Fenglian Chen
Yanling Hao
author_facet Zhiqi Wang
Zhaosen Yuan
Xinlai Dou
Wanshan Yang
Huining Zhang
Yue Zhang
Fenglian Chen
Yanling Hao
author_sort Zhiqi Wang
collection DOAJ
description This study investigated the effects of <i>Lactococcus lactis</i> subsp. 1.2472 (L)-, <i>Streptococcus thermophilus</i> 1.2718 (S)-, and thermostable <i>Lactobacillus rhamnosus</i> HCUL 1.1901-1912 (T)-fermented rice flour with inoculum levels of 3–11% (<i>w</i>/<i>w</i>) on rice bread staling. Optimal staling resistance was achieved, as follows: 9% L-fermented rice bread (LRB), 7% T-fermented rice bread (TRB), and 5% S-fermented rice bread (SRB). Lactic acid bacteria-fermented rice flour significantly enhanced hydration properties. LF-NMR analysis revealed that T<sub>21</sub> (strongly bound water) and T<sub>22</sub> (weakly bound water) relaxation times decreased, while T<sub>23</sub> (free water) increased with prolonged storage. Fermented-rice-flour groups had significantly more strongly bound water than the control group on 7 d. The optimized formulations exhibited exceptional volumetric stability with specific volume change rates of 17.63% (LRB), 17.60% (TRB), and 19.58% (SRB), coupled with maximal porosities of 10.34%, 9.05%, and 9.41%, respectively. This study provides a theoretical foundation for improving rice bread’s anti-staling properties.
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id doaj-art-c43bbf589ca84d29bc3a8b5e3b368414
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issn 2304-8158
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publishDate 2025-07-01
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spelling doaj-art-c43bbf589ca84d29bc3a8b5e3b3684142025-08-20T03:02:49ZengMDPI AGFoods2304-81582025-07-011415267410.3390/foods14152674Enhancement of Anti-Staling Properties of Rice Bread Through Fermentation Rice Flour with Three Lactic Acid BacteriaZhiqi Wang0Zhaosen Yuan1Xinlai Dou2Wanshan Yang3Huining Zhang4Yue Zhang5Fenglian Chen6Yanling Hao7College of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaCollege of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaInstitute of Nutrition and Health, China Agricultural University, Beijing 100083, ChinaCollege of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaCollege of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaCollege of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaCollege of Food Engineering, Harbin University of Commerce, Harbin 150028, ChinaInstitute of Nutrition and Health, China Agricultural University, Beijing 100083, ChinaThis study investigated the effects of <i>Lactococcus lactis</i> subsp. 1.2472 (L)-, <i>Streptococcus thermophilus</i> 1.2718 (S)-, and thermostable <i>Lactobacillus rhamnosus</i> HCUL 1.1901-1912 (T)-fermented rice flour with inoculum levels of 3–11% (<i>w</i>/<i>w</i>) on rice bread staling. Optimal staling resistance was achieved, as follows: 9% L-fermented rice bread (LRB), 7% T-fermented rice bread (TRB), and 5% S-fermented rice bread (SRB). Lactic acid bacteria-fermented rice flour significantly enhanced hydration properties. LF-NMR analysis revealed that T<sub>21</sub> (strongly bound water) and T<sub>22</sub> (weakly bound water) relaxation times decreased, while T<sub>23</sub> (free water) increased with prolonged storage. Fermented-rice-flour groups had significantly more strongly bound water than the control group on 7 d. The optimized formulations exhibited exceptional volumetric stability with specific volume change rates of 17.63% (LRB), 17.60% (TRB), and 19.58% (SRB), coupled with maximal porosities of 10.34%, 9.05%, and 9.41%, respectively. This study provides a theoretical foundation for improving rice bread’s anti-staling properties.https://www.mdpi.com/2304-8158/14/15/2674rice breadanti-stalinglactic acid bacteriafermented rice flour
spellingShingle Zhiqi Wang
Zhaosen Yuan
Xinlai Dou
Wanshan Yang
Huining Zhang
Yue Zhang
Fenglian Chen
Yanling Hao
Enhancement of Anti-Staling Properties of Rice Bread Through Fermentation Rice Flour with Three Lactic Acid Bacteria
Foods
rice bread
anti-staling
lactic acid bacteria
fermented rice flour
title Enhancement of Anti-Staling Properties of Rice Bread Through Fermentation Rice Flour with Three Lactic Acid Bacteria
title_full Enhancement of Anti-Staling Properties of Rice Bread Through Fermentation Rice Flour with Three Lactic Acid Bacteria
title_fullStr Enhancement of Anti-Staling Properties of Rice Bread Through Fermentation Rice Flour with Three Lactic Acid Bacteria
title_full_unstemmed Enhancement of Anti-Staling Properties of Rice Bread Through Fermentation Rice Flour with Three Lactic Acid Bacteria
title_short Enhancement of Anti-Staling Properties of Rice Bread Through Fermentation Rice Flour with Three Lactic Acid Bacteria
title_sort enhancement of anti staling properties of rice bread through fermentation rice flour with three lactic acid bacteria
topic rice bread
anti-staling
lactic acid bacteria
fermented rice flour
url https://www.mdpi.com/2304-8158/14/15/2674
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