Significance of photosystem integrity and reducing Cd accumulation in mitigating Cd stress on olive tree inoculated with soil-borne bacteria consortium is variety-dependent
Rhizosphere microbial community role in boosting fruit tree ability to cope with heavy metals is gaining interest. Here, we address the effects of in-frame holobiont microbial inoculation on two Tunisian olive tree varieties (Olea europaea L. cv. Chemlali and Chetoui) exposed to CdCl2 for one month....
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Plant Stress |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X25000533 |
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| author | Rabaa Hidri Rahma Azri Katja Burow Walid Zorrig Ahmed Debez Nouf M. Alyami Mokded Rabhi Asma Bensalem-Fnayou Ahmed Mliki Philipp Franken Ricardo Aroca |
| author_facet | Rabaa Hidri Rahma Azri Katja Burow Walid Zorrig Ahmed Debez Nouf M. Alyami Mokded Rabhi Asma Bensalem-Fnayou Ahmed Mliki Philipp Franken Ricardo Aroca |
| author_sort | Rabaa Hidri |
| collection | DOAJ |
| description | Rhizosphere microbial community role in boosting fruit tree ability to cope with heavy metals is gaining interest. Here, we address the effects of in-frame holobiont microbial inoculation on two Tunisian olive tree varieties (Olea europaea L. cv. Chemlali and Chetoui) exposed to CdCl2 for one month. Shoot Cd content, leaf pigment contents, chlorophyll fluorescence, and energy conversion-related traits in photosystems I and II (PSI and PSII) were considered. Following pyrosequencing-based 16S rRNA analysis, Proteobacteria, and to a lesser extent Firmicutes phylums, were the most abundant. Both Cd-treated olive varieties showed a significant decrease in leaf pigment contents, however, this depressive effect was significantly mitigated following inoculation. PSI and PSII activities were more altered in Cd-treated Chetoui as compared to Chemlali. Quantum yield and electron transport of PSI and PSII were also more impacted in Chetoui, whereas the limitation of the donor side of PSI increased in the latter. Yet, Cd impact on these parameters was significantly reduced in inoculated plants. The quantum yield of nonregulated non-photochemical energy loss in PSII significantly increased in both olive varieties under Cd stress, but values were markedly restored with inoculation. High Cd accumulation observed in Chetoui shoots upon Cd exposure was significantly lower in inoculated plants. Overall, the consortium used improved olive tree Cd tolerance likely due to the restoration of leaf pigment contents and the better integrity of photosystems in both varieties, whereas lowering Cd translocation to shoots would be more prominent in Chetoui. Long-term experiments will be conducted to confirm these results. |
| format | Article |
| id | doaj-art-c43730e8847a49619ffefde70ca3a6a1 |
| institution | DOAJ |
| issn | 2667-064X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj-art-c43730e8847a49619ffefde70ca3a6a12025-08-20T02:56:33ZengElsevierPlant Stress2667-064X2025-03-011510078810.1016/j.stress.2025.100788Significance of photosystem integrity and reducing Cd accumulation in mitigating Cd stress on olive tree inoculated with soil-borne bacteria consortium is variety-dependentRabaa Hidri0Rahma Azri1Katja Burow2Walid Zorrig3Ahmed Debez4Nouf M. Alyami5Mokded Rabhi6Asma Bensalem-Fnayou7Ahmed Mliki8Philipp Franken9Ricardo Aroca10Laboratory of Extremophile Plants, Centre of Biotechnology of Borj-Cedria, P.O. Box 901, Hammam-Lif 2050, Tunisia; Corresponding author.Laboratory of Plant Molecular Physiology, Centre of Biotechnology of Borj-Cedria, P.O. Box 901, Hammam-Lif 2050, TunisiaErfurt Research Centre for Horticultural Crops, University of Applied Sciences, Kühnhäuser Str. 101, 99090 Erfurt, Thuringia, GermanyDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaLaboratory of Extremophile Plants, Centre of Biotechnology of Borj-Cedria, P.O. Box 901, Hammam-Lif 2050, TunisiaDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Plant Production and Protection, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah, Saudi ArabiaLaboratory of Plant Molecular Physiology, Centre of Biotechnology of Borj-Cedria, P.O. Box 901, Hammam-Lif 2050, TunisiaLaboratory of Plant Molecular Physiology, Centre of Biotechnology of Borj-Cedria, P.O. Box 901, Hammam-Lif 2050, TunisiaErfurt Research Centre for Horticultural Crops, University of Applied Sciences, Kühnhäuser Str. 101, 99090 Erfurt, Thuringia, GermanyDepartamento de Microbiología del Suelo y la Planta, Estación Experimental del Zaidín (CSIC), C/ Profesor Albareda 1, 18008 Granada, SpainRhizosphere microbial community role in boosting fruit tree ability to cope with heavy metals is gaining interest. Here, we address the effects of in-frame holobiont microbial inoculation on two Tunisian olive tree varieties (Olea europaea L. cv. Chemlali and Chetoui) exposed to CdCl2 for one month. Shoot Cd content, leaf pigment contents, chlorophyll fluorescence, and energy conversion-related traits in photosystems I and II (PSI and PSII) were considered. Following pyrosequencing-based 16S rRNA analysis, Proteobacteria, and to a lesser extent Firmicutes phylums, were the most abundant. Both Cd-treated olive varieties showed a significant decrease in leaf pigment contents, however, this depressive effect was significantly mitigated following inoculation. PSI and PSII activities were more altered in Cd-treated Chetoui as compared to Chemlali. Quantum yield and electron transport of PSI and PSII were also more impacted in Chetoui, whereas the limitation of the donor side of PSI increased in the latter. Yet, Cd impact on these parameters was significantly reduced in inoculated plants. The quantum yield of nonregulated non-photochemical energy loss in PSII significantly increased in both olive varieties under Cd stress, but values were markedly restored with inoculation. High Cd accumulation observed in Chetoui shoots upon Cd exposure was significantly lower in inoculated plants. Overall, the consortium used improved olive tree Cd tolerance likely due to the restoration of leaf pigment contents and the better integrity of photosystems in both varieties, whereas lowering Cd translocation to shoots would be more prominent in Chetoui. Long-term experiments will be conducted to confirm these results.http://www.sciencedirect.com/science/article/pii/S2667064X25000533Cadmium stressMicrobial consortiumBacteriaOlive varietiesPhotosystem activitiesCd accumulation |
| spellingShingle | Rabaa Hidri Rahma Azri Katja Burow Walid Zorrig Ahmed Debez Nouf M. Alyami Mokded Rabhi Asma Bensalem-Fnayou Ahmed Mliki Philipp Franken Ricardo Aroca Significance of photosystem integrity and reducing Cd accumulation in mitigating Cd stress on olive tree inoculated with soil-borne bacteria consortium is variety-dependent Plant Stress Cadmium stress Microbial consortium Bacteria Olive varieties Photosystem activities Cd accumulation |
| title | Significance of photosystem integrity and reducing Cd accumulation in mitigating Cd stress on olive tree inoculated with soil-borne bacteria consortium is variety-dependent |
| title_full | Significance of photosystem integrity and reducing Cd accumulation in mitigating Cd stress on olive tree inoculated with soil-borne bacteria consortium is variety-dependent |
| title_fullStr | Significance of photosystem integrity and reducing Cd accumulation in mitigating Cd stress on olive tree inoculated with soil-borne bacteria consortium is variety-dependent |
| title_full_unstemmed | Significance of photosystem integrity and reducing Cd accumulation in mitigating Cd stress on olive tree inoculated with soil-borne bacteria consortium is variety-dependent |
| title_short | Significance of photosystem integrity and reducing Cd accumulation in mitigating Cd stress on olive tree inoculated with soil-borne bacteria consortium is variety-dependent |
| title_sort | significance of photosystem integrity and reducing cd accumulation in mitigating cd stress on olive tree inoculated with soil borne bacteria consortium is variety dependent |
| topic | Cadmium stress Microbial consortium Bacteria Olive varieties Photosystem activities Cd accumulation |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X25000533 |
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