Osteogenic Differentiated Human Bone Marrow Stem Cells Contribute to Sprouting Angiogenesis Deceleration via Paracrine Excreted IGFBP7
Abstract Deceleration of sprouting angiogenesis until its final disappearance after blood vessel reconstruction is crucial for controlled tissue repair; however, its underlying mechanism remains unclear. It is reported that osteogenic differentiated bone marrow stem cells (OD‐BMSCs) contribute to sp...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2022-12-01
|
| Series: | Advanced Materials Interfaces |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/admi.202201719 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849425765920145408 |
|---|---|
| author | Shiyu Sun Cen Cao Yaru Guo Yi Hou Yanze Meng Yuman Li Feng Mei Zhewen Hu Yao Li Yuting Niu Yiming Chen Lili Chen Ying Huang Xuliang Deng |
| author_facet | Shiyu Sun Cen Cao Yaru Guo Yi Hou Yanze Meng Yuman Li Feng Mei Zhewen Hu Yao Li Yuting Niu Yiming Chen Lili Chen Ying Huang Xuliang Deng |
| author_sort | Shiyu Sun |
| collection | DOAJ |
| description | Abstract Deceleration of sprouting angiogenesis until its final disappearance after blood vessel reconstruction is crucial for controlled tissue repair; however, its underlying mechanism remains unclear. It is reported that osteogenic differentiated bone marrow stem cells (OD‐BMSCs) contribute to sprouting angiogenesis deceleration by releasing intrinsic “OFF” signals. In vitro experiments show that insulin‐like growth factor‐binding protein 7 (IGFBP7) is the main component of OD‐BMSCs paracrine products which could inhibit the tube formation ability of endothelial cells. In addition, cell functional experiments show that IGFBP7 inhibits sprouting angiogenesis by reducing cell migration and tip cell specification. Furthermore, it is found that early IGFBP7 intervention, which accelerates sprouting angiogenesis deceleration during the early stage of healing, impedes bone defect healing. These results demonstrate that OD‐BMSCs could offer intrinsic inhibitory signals on sprouting angiogenesis and the appropriate emergence timing of these signals is crucial to maintain vasculature homeostasis during bone repairing. These results provide insight into the complex interaction between osteogenesis–angiogenesis coupling and suggest the potential therapeutic application of IGFBP7 in regulating vascular homeostasis. |
| format | Article |
| id | doaj-art-c42e261424244f8099efdd886ed8aa29 |
| institution | Kabale University |
| issn | 2196-7350 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj-art-c42e261424244f8099efdd886ed8aa292025-08-20T03:29:39ZengWiley-VCHAdvanced Materials Interfaces2196-73502022-12-01935n/an/a10.1002/admi.202201719Osteogenic Differentiated Human Bone Marrow Stem Cells Contribute to Sprouting Angiogenesis Deceleration via Paracrine Excreted IGFBP7Shiyu Sun0Cen Cao1Yaru Guo2Yi Hou3Yanze Meng4Yuman Li5Feng Mei6Zhewen Hu7Yao Li8Yuting Niu9Yiming Chen10Lili Chen11Ying Huang12Xuliang Deng13Beijing Laboratory of Biomedical Materials Department of Geriatric Dentistry Peking University School and Hospital of Stomatology Beijing 100081 ChinaDepartment of Stomatology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 ChinaBeijing Laboratory of Biomedical Materials Department of Geriatric Dentistry Peking University School and Hospital of Stomatology Beijing 100081 ChinaCollege of Life Science and Technology Beijing University of Chemical Technology Beijing 100029 ChinaBeijing Laboratory of Biomedical Materials Department of Geriatric Dentistry Peking University School and Hospital of Stomatology Beijing 100081 ChinaBeijing Laboratory of Biomedical Materials Department of Geriatric Dentistry Peking University School and Hospital of Stomatology Beijing 100081 ChinaDepartment of Stomatology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 ChinaBeijing Laboratory of Biomedical Materials Department of Geriatric Dentistry Peking University School and Hospital of Stomatology Beijing 100081 ChinaBeijing Laboratory of Biomedical Materials Department of Geriatric Dentistry Peking University School and Hospital of Stomatology Beijing 100081 ChinaBeijing Laboratory of Biomedical Materials Department of Geriatric Dentistry Peking University School and Hospital of Stomatology Beijing 100081 ChinaBeijing Laboratory of Biomedical Materials Department of Geriatric Dentistry Peking University School and Hospital of Stomatology Beijing 100081 ChinaDepartment of Stomatology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 ChinaBeijing Laboratory of Biomedical Materials Department of Geriatric Dentistry Peking University School and Hospital of Stomatology Beijing 100081 ChinaBeijing Laboratory of Biomedical Materials Department of Geriatric Dentistry Peking University School and Hospital of Stomatology Beijing 100081 ChinaAbstract Deceleration of sprouting angiogenesis until its final disappearance after blood vessel reconstruction is crucial for controlled tissue repair; however, its underlying mechanism remains unclear. It is reported that osteogenic differentiated bone marrow stem cells (OD‐BMSCs) contribute to sprouting angiogenesis deceleration by releasing intrinsic “OFF” signals. In vitro experiments show that insulin‐like growth factor‐binding protein 7 (IGFBP7) is the main component of OD‐BMSCs paracrine products which could inhibit the tube formation ability of endothelial cells. In addition, cell functional experiments show that IGFBP7 inhibits sprouting angiogenesis by reducing cell migration and tip cell specification. Furthermore, it is found that early IGFBP7 intervention, which accelerates sprouting angiogenesis deceleration during the early stage of healing, impedes bone defect healing. These results demonstrate that OD‐BMSCs could offer intrinsic inhibitory signals on sprouting angiogenesis and the appropriate emergence timing of these signals is crucial to maintain vasculature homeostasis during bone repairing. These results provide insight into the complex interaction between osteogenesis–angiogenesis coupling and suggest the potential therapeutic application of IGFBP7 in regulating vascular homeostasis.https://doi.org/10.1002/admi.202201719endothelial cells (ECs)insulin‐like growth factor‐binding protein 7 (IGFBP7)osteogenic differentiated bone marrow stem cells (BMSCs)paracrine effectsprouting angiogenesis deceleration |
| spellingShingle | Shiyu Sun Cen Cao Yaru Guo Yi Hou Yanze Meng Yuman Li Feng Mei Zhewen Hu Yao Li Yuting Niu Yiming Chen Lili Chen Ying Huang Xuliang Deng Osteogenic Differentiated Human Bone Marrow Stem Cells Contribute to Sprouting Angiogenesis Deceleration via Paracrine Excreted IGFBP7 Advanced Materials Interfaces endothelial cells (ECs) insulin‐like growth factor‐binding protein 7 (IGFBP7) osteogenic differentiated bone marrow stem cells (BMSCs) paracrine effect sprouting angiogenesis deceleration |
| title | Osteogenic Differentiated Human Bone Marrow Stem Cells Contribute to Sprouting Angiogenesis Deceleration via Paracrine Excreted IGFBP7 |
| title_full | Osteogenic Differentiated Human Bone Marrow Stem Cells Contribute to Sprouting Angiogenesis Deceleration via Paracrine Excreted IGFBP7 |
| title_fullStr | Osteogenic Differentiated Human Bone Marrow Stem Cells Contribute to Sprouting Angiogenesis Deceleration via Paracrine Excreted IGFBP7 |
| title_full_unstemmed | Osteogenic Differentiated Human Bone Marrow Stem Cells Contribute to Sprouting Angiogenesis Deceleration via Paracrine Excreted IGFBP7 |
| title_short | Osteogenic Differentiated Human Bone Marrow Stem Cells Contribute to Sprouting Angiogenesis Deceleration via Paracrine Excreted IGFBP7 |
| title_sort | osteogenic differentiated human bone marrow stem cells contribute to sprouting angiogenesis deceleration via paracrine excreted igfbp7 |
| topic | endothelial cells (ECs) insulin‐like growth factor‐binding protein 7 (IGFBP7) osteogenic differentiated bone marrow stem cells (BMSCs) paracrine effect sprouting angiogenesis deceleration |
| url | https://doi.org/10.1002/admi.202201719 |
| work_keys_str_mv | AT shiyusun osteogenicdifferentiatedhumanbonemarrowstemcellscontributetosproutingangiogenesisdecelerationviaparacrineexcretedigfbp7 AT cencao osteogenicdifferentiatedhumanbonemarrowstemcellscontributetosproutingangiogenesisdecelerationviaparacrineexcretedigfbp7 AT yaruguo osteogenicdifferentiatedhumanbonemarrowstemcellscontributetosproutingangiogenesisdecelerationviaparacrineexcretedigfbp7 AT yihou osteogenicdifferentiatedhumanbonemarrowstemcellscontributetosproutingangiogenesisdecelerationviaparacrineexcretedigfbp7 AT yanzemeng osteogenicdifferentiatedhumanbonemarrowstemcellscontributetosproutingangiogenesisdecelerationviaparacrineexcretedigfbp7 AT yumanli osteogenicdifferentiatedhumanbonemarrowstemcellscontributetosproutingangiogenesisdecelerationviaparacrineexcretedigfbp7 AT fengmei osteogenicdifferentiatedhumanbonemarrowstemcellscontributetosproutingangiogenesisdecelerationviaparacrineexcretedigfbp7 AT zhewenhu osteogenicdifferentiatedhumanbonemarrowstemcellscontributetosproutingangiogenesisdecelerationviaparacrineexcretedigfbp7 AT yaoli osteogenicdifferentiatedhumanbonemarrowstemcellscontributetosproutingangiogenesisdecelerationviaparacrineexcretedigfbp7 AT yutingniu osteogenicdifferentiatedhumanbonemarrowstemcellscontributetosproutingangiogenesisdecelerationviaparacrineexcretedigfbp7 AT yimingchen osteogenicdifferentiatedhumanbonemarrowstemcellscontributetosproutingangiogenesisdecelerationviaparacrineexcretedigfbp7 AT lilichen osteogenicdifferentiatedhumanbonemarrowstemcellscontributetosproutingangiogenesisdecelerationviaparacrineexcretedigfbp7 AT yinghuang osteogenicdifferentiatedhumanbonemarrowstemcellscontributetosproutingangiogenesisdecelerationviaparacrineexcretedigfbp7 AT xuliangdeng osteogenicdifferentiatedhumanbonemarrowstemcellscontributetosproutingangiogenesisdecelerationviaparacrineexcretedigfbp7 |