Aptamer-dependent full-length cDNA synthesis by overlap extension PCR

Sequencing of the human genome in combination with computational annotation has provided tremendous data on predicted genes. However, for most of them, no corresponding cDNAs are available yet. Furthermore, even where cDNA clones were obtained, gene transcripts often have many different splice varia...

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Bibliographic Details
Main Authors: Yasumasa Mitani, Takayuki Nakayama, Matthias Harbers, Yoshihide Hayashizaki
Format: Article
Language:English
Published: Taylor & Francis Group 2004-07-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/04371DD02
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