Engineered Tissue Models to Decode Host–Microbiota Interactions
Abstract A mutualistic co‐evolution exists between the host and its associated microbiota in the human body. Bacteria establish ecological niches in various tissues of the body, locally influencing their physiology and functions, but also contributing to the well‐being of the whole organism through...
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| Format: | Article |
| Language: | English |
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Wiley
2025-06-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202417687 |
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| author | Miryam Adelfio Grace E. Callen Xuesong He Bruce J. Paster Hatice Hasturk Chiara E. Ghezzi |
| author_facet | Miryam Adelfio Grace E. Callen Xuesong He Bruce J. Paster Hatice Hasturk Chiara E. Ghezzi |
| author_sort | Miryam Adelfio |
| collection | DOAJ |
| description | Abstract A mutualistic co‐evolution exists between the host and its associated microbiota in the human body. Bacteria establish ecological niches in various tissues of the body, locally influencing their physiology and functions, but also contributing to the well‐being of the whole organism through systemic communication with other distant niches (axis). Emerging evidence indicates that when the composition of the microbiota inhabiting the niche changes toward a pathogenic state (dysbiosis) and interactions with the host become unbalanced, diseases may present. In addition, imbalances within a single niche can cause dysbiosis in distant organs. Current research efforts are focused on elucidating the mechanisms leading to dysbiosis, with the goal of restoring tissue homeostasis. In vitro models can provide critical experimental platforms to address this need, by reproducing the niche cyto‐architecture and physiology with high fidelity. This review surveys current in in vitro host–microbiota research strategies and provides a roadmap that can guide the field in further developing physiologically relevant in vitro models of ecological niches, thus enabling investigation of the role of the microbiota in human health and diseases. Lastly, given the Food and Drug Administration Modernization Act 2.0, this review highlights emerging in vitro strategies to support the development and validation of new therapies on the market. |
| format | Article |
| id | doaj-art-c42a7d007fc64b0289c447a5cf598a8a |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-c42a7d007fc64b0289c447a5cf598a8a2025-08-20T03:31:26ZengWileyAdvanced Science2198-38442025-06-011223n/an/a10.1002/advs.202417687Engineered Tissue Models to Decode Host–Microbiota InteractionsMiryam Adelfio0Grace E. Callen1Xuesong He2Bruce J. Paster3Hatice Hasturk4Chiara E. Ghezzi5Department of Biomedical Engineering University of Massachusetts‐Lowell Lowell MA 01854 USADepartment of Biomedical Engineering University of Massachusetts‐Lowell Lowell MA 01854 USAADA Forsyth Institute 245 First St Cambridge MA 02142 USAADA Forsyth Institute 245 First St Cambridge MA 02142 USAADA Forsyth Institute 245 First St Cambridge MA 02142 USADepartment of Biomedical Engineering University of Massachusetts‐Lowell Lowell MA 01854 USAAbstract A mutualistic co‐evolution exists between the host and its associated microbiota in the human body. Bacteria establish ecological niches in various tissues of the body, locally influencing their physiology and functions, but also contributing to the well‐being of the whole organism through systemic communication with other distant niches (axis). Emerging evidence indicates that when the composition of the microbiota inhabiting the niche changes toward a pathogenic state (dysbiosis) and interactions with the host become unbalanced, diseases may present. In addition, imbalances within a single niche can cause dysbiosis in distant organs. Current research efforts are focused on elucidating the mechanisms leading to dysbiosis, with the goal of restoring tissue homeostasis. In vitro models can provide critical experimental platforms to address this need, by reproducing the niche cyto‐architecture and physiology with high fidelity. This review surveys current in in vitro host–microbiota research strategies and provides a roadmap that can guide the field in further developing physiologically relevant in vitro models of ecological niches, thus enabling investigation of the role of the microbiota in human health and diseases. Lastly, given the Food and Drug Administration Modernization Act 2.0, this review highlights emerging in vitro strategies to support the development and validation of new therapies on the market.https://doi.org/10.1002/advs.202417687dysbiosisfemale reproductive tracthost‐microbiome interactionsintestineoralskin |
| spellingShingle | Miryam Adelfio Grace E. Callen Xuesong He Bruce J. Paster Hatice Hasturk Chiara E. Ghezzi Engineered Tissue Models to Decode Host–Microbiota Interactions Advanced Science dysbiosis female reproductive tract host‐microbiome interactions intestine oral skin |
| title | Engineered Tissue Models to Decode Host–Microbiota Interactions |
| title_full | Engineered Tissue Models to Decode Host–Microbiota Interactions |
| title_fullStr | Engineered Tissue Models to Decode Host–Microbiota Interactions |
| title_full_unstemmed | Engineered Tissue Models to Decode Host–Microbiota Interactions |
| title_short | Engineered Tissue Models to Decode Host–Microbiota Interactions |
| title_sort | engineered tissue models to decode host microbiota interactions |
| topic | dysbiosis female reproductive tract host‐microbiome interactions intestine oral skin |
| url | https://doi.org/10.1002/advs.202417687 |
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