A novel p.127Val>Ile single nucleotide polymorphism in the gene and its relation to litter size in Thin-tailed Indonesian ewes
Objective The primary objective was to identify and characterize the single nucleotide polymorphisms (SNPs) within the MTNR1A gene sequence in Thin-tailed Indonesian ewes to assess the possible association of MTNR1A gene polymorphism with litter size trait. Methods Forty-seven Thin-tailed Indonesian...
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Asian-Australasian Association of Animal Production Societies
2025-02-01
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| Series: | Animal Bioscience |
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| Online Access: | http://www.animbiosci.org/upload/pdf/ab-24-0187.pdf |
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| author | Mutasem Abuzahra Mohammed Baqur S. Al-Shuhaib Dwi Wijayanti Mustofa Helmi Effendi Imam Mustofa Ikechukwu Benjamin Moses |
| author_facet | Mutasem Abuzahra Mohammed Baqur S. Al-Shuhaib Dwi Wijayanti Mustofa Helmi Effendi Imam Mustofa Ikechukwu Benjamin Moses |
| author_sort | Mutasem Abuzahra |
| collection | DOAJ |
| description | Objective The primary objective was to identify and characterize the single nucleotide polymorphisms (SNPs) within the MTNR1A gene sequence in Thin-tailed Indonesian ewes to assess the possible association of MTNR1A gene polymorphism with litter size trait. Methods Forty-seven Thin-tailed Indonesian sheep were selected for the study. Genotyping involved collecting blood samples, and sequencing exon 2 of the MTNR1A gene. Results The study identified 19 novel SNPs, with 10 being non-synonymous variations, in the MTNR1A gene of Thin-tailed Indonesian ewes. One non-synonymous SNP (rs1087815963) showed a significant association with litter size, with the GC genotype exhibiting a higher average litter size than the GG genotype. The deleterious impact of p.Val127Ile SNP was predicted by various in silico tools that predicted a highly damaging effect of p.Val127Ile SNP on the structure, function, and stability of MTNR1A. Docking reactions showed a critical involvement of this locus with the binding with melatonin. Conclusion In conclusion, the results of our study suggest that rs1087815963 has a remarkable negative impact on the MTNR1A with a putative alteration in the binding with melatonin. Therefore, the implementation of the novel p.Val127Ile could be a useful marker in marker-assisted selection. |
| format | Article |
| id | doaj-art-c42a38d6a4e9410ebbc8473fd81304c6 |
| institution | DOAJ |
| issn | 2765-0189 2765-0235 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj-art-c42a38d6a4e9410ebbc8473fd81304c62025-08-20T02:43:10ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352025-02-0138220922210.5713/ab.24.018725283A novel p.127Val>Ile single nucleotide polymorphism in the gene and its relation to litter size in Thin-tailed Indonesian ewesMutasem Abuzahra0Mohammed Baqur S. Al-Shuhaib1Dwi Wijayanti2Mustofa Helmi Effendi3Imam Mustofa4Ikechukwu Benjamin Moses5 Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia Department of Animal Production, College of Agriculture, Al-Qasim Green University, Al-Qasim, 51013, Babil, Iraq College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115, Indonesia Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki 481101, NigeriaObjective The primary objective was to identify and characterize the single nucleotide polymorphisms (SNPs) within the MTNR1A gene sequence in Thin-tailed Indonesian ewes to assess the possible association of MTNR1A gene polymorphism with litter size trait. Methods Forty-seven Thin-tailed Indonesian sheep were selected for the study. Genotyping involved collecting blood samples, and sequencing exon 2 of the MTNR1A gene. Results The study identified 19 novel SNPs, with 10 being non-synonymous variations, in the MTNR1A gene of Thin-tailed Indonesian ewes. One non-synonymous SNP (rs1087815963) showed a significant association with litter size, with the GC genotype exhibiting a higher average litter size than the GG genotype. The deleterious impact of p.Val127Ile SNP was predicted by various in silico tools that predicted a highly damaging effect of p.Val127Ile SNP on the structure, function, and stability of MTNR1A. Docking reactions showed a critical involvement of this locus with the binding with melatonin. Conclusion In conclusion, the results of our study suggest that rs1087815963 has a remarkable negative impact on the MTNR1A with a putative alteration in the binding with melatonin. Therefore, the implementation of the novel p.Val127Ile could be a useful marker in marker-assisted selection.http://www.animbiosci.org/upload/pdf/ab-24-0187.pdfgenetic diversity and farmed animalslitter sizesingle nucleotide polymorphisms |
| spellingShingle | Mutasem Abuzahra Mohammed Baqur S. Al-Shuhaib Dwi Wijayanti Mustofa Helmi Effendi Imam Mustofa Ikechukwu Benjamin Moses A novel p.127Val>Ile single nucleotide polymorphism in the gene and its relation to litter size in Thin-tailed Indonesian ewes Animal Bioscience genetic diversity and farmed animals litter size single nucleotide polymorphisms |
| title | A novel p.127Val>Ile single nucleotide polymorphism in the gene and its relation to litter size in Thin-tailed Indonesian ewes |
| title_full | A novel p.127Val>Ile single nucleotide polymorphism in the gene and its relation to litter size in Thin-tailed Indonesian ewes |
| title_fullStr | A novel p.127Val>Ile single nucleotide polymorphism in the gene and its relation to litter size in Thin-tailed Indonesian ewes |
| title_full_unstemmed | A novel p.127Val>Ile single nucleotide polymorphism in the gene and its relation to litter size in Thin-tailed Indonesian ewes |
| title_short | A novel p.127Val>Ile single nucleotide polymorphism in the gene and its relation to litter size in Thin-tailed Indonesian ewes |
| title_sort | novel p 127val ile single nucleotide polymorphism in the gene and its relation to litter size in thin tailed indonesian ewes |
| topic | genetic diversity and farmed animals litter size single nucleotide polymorphisms |
| url | http://www.animbiosci.org/upload/pdf/ab-24-0187.pdf |
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