Formulation, characterization and co-delivery of curcumin-rosemary loaded niosomes to enhance antimicrobial activity against staphylococcus aureus strains
Objectives: Staphylococcus aureus is a common human pathogen that infects thousands of people every year. The growing resistance of this bacterium to antibiotics has increased the need for developing more effective and natural antimicrobial medications. In this study, niosomes loaded Rosmarinus offi...
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Mashhad University of Medical Sciences
2025-04-01
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| Series: | Nanomedicine Journal |
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| Online Access: | https://nmj.mums.ac.ir/article_25432_a8580205aec4fd19151912ee3f73d716.pdf |
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| author | Elmira Mehdipour Alireza Iranbakhsh Amir Mirzaie |
| author_facet | Elmira Mehdipour Alireza Iranbakhsh Amir Mirzaie |
| author_sort | Elmira Mehdipour |
| collection | DOAJ |
| description | Objectives: Staphylococcus aureus is a common human pathogen that infects thousands of people every year. The growing resistance of this bacterium to antibiotics has increased the need for developing more effective and natural antimicrobial medications. In this study, niosomes loaded Rosmarinus officinalis (rosemary)@curcumin were synthesized, and their antimicrobial and anti-biofilm activity on clinical S. aureus strains was investigated.Materials and Methods: Rosmarinus officinalis (rosemary) extract was prepared through maceration and its compounds were identified by GC/MS. Niosomes loaded rosemary@curcumin were synthesized through thin-film hydration. Their characteristics were analyzed with dynamic light scattering (DLS)-zetasizer, scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and drug release test was studied using a dialysis bag. Finally, the antimicrobial and anti-biofilm activity against clinical strains of S. aureus were investigated using minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and crystal violet methods, respectively.Results: Formulated niosomes were spherical, averaged 442 nm in size, and had uniform particle distribution. FTIR results indicated the successful encapsulation of rosemary@curcumin inside niosomes with 94.23% encapsulation efficiency. In vitro drug release studies showed a slow-release pattern of rosemary@curcumin from niosomes. The MIC and MBC values of niosomes loaded rosemary@curcumin were between 32.5 and 62.5 µg/ml and showed higher antimicrobial activity. Also, the results of the biofilm inhibition test showed that niosomes loaded rosemary@curcumin reduced the rate of biofilm formation between 2 and 4 fold.Conclusion: Niosomes loaded rosemary@curcumin have suitable structure and surface characteristics and can successfully inhibit S. aureus growth and prevent its biofilm formation potency. |
| format | Article |
| id | doaj-art-c4216a1c19eb4116b13b8dfcf11e14ae |
| institution | Kabale University |
| issn | 2322-3049 2322-5904 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Nanomedicine Journal |
| spelling | doaj-art-c4216a1c19eb4116b13b8dfcf11e14ae2025-08-20T03:35:06ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042025-04-0112231332310.22038/nmj.2025.78286.191425432Formulation, characterization and co-delivery of curcumin-rosemary loaded niosomes to enhance antimicrobial activity against staphylococcus aureus strainsElmira Mehdipour0Alireza Iranbakhsh1Amir Mirzaie2Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Biology, Parand Branch, Islamic Azad University, Parand, IranObjectives: Staphylococcus aureus is a common human pathogen that infects thousands of people every year. The growing resistance of this bacterium to antibiotics has increased the need for developing more effective and natural antimicrobial medications. In this study, niosomes loaded Rosmarinus officinalis (rosemary)@curcumin were synthesized, and their antimicrobial and anti-biofilm activity on clinical S. aureus strains was investigated.Materials and Methods: Rosmarinus officinalis (rosemary) extract was prepared through maceration and its compounds were identified by GC/MS. Niosomes loaded rosemary@curcumin were synthesized through thin-film hydration. Their characteristics were analyzed with dynamic light scattering (DLS)-zetasizer, scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and drug release test was studied using a dialysis bag. Finally, the antimicrobial and anti-biofilm activity against clinical strains of S. aureus were investigated using minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and crystal violet methods, respectively.Results: Formulated niosomes were spherical, averaged 442 nm in size, and had uniform particle distribution. FTIR results indicated the successful encapsulation of rosemary@curcumin inside niosomes with 94.23% encapsulation efficiency. In vitro drug release studies showed a slow-release pattern of rosemary@curcumin from niosomes. The MIC and MBC values of niosomes loaded rosemary@curcumin were between 32.5 and 62.5 µg/ml and showed higher antimicrobial activity. Also, the results of the biofilm inhibition test showed that niosomes loaded rosemary@curcumin reduced the rate of biofilm formation between 2 and 4 fold.Conclusion: Niosomes loaded rosemary@curcumin have suitable structure and surface characteristics and can successfully inhibit S. aureus growth and prevent its biofilm formation potency.https://nmj.mums.ac.ir/article_25432_a8580205aec4fd19151912ee3f73d716.pdfantimicrobialcurcuminniosomerosmarinus officinalisstaphylococcus aureus |
| spellingShingle | Elmira Mehdipour Alireza Iranbakhsh Amir Mirzaie Formulation, characterization and co-delivery of curcumin-rosemary loaded niosomes to enhance antimicrobial activity against staphylococcus aureus strains Nanomedicine Journal antimicrobial curcumin niosome rosmarinus officinalis staphylococcus aureus |
| title | Formulation, characterization and co-delivery of curcumin-rosemary loaded niosomes to enhance antimicrobial activity against staphylococcus aureus strains |
| title_full | Formulation, characterization and co-delivery of curcumin-rosemary loaded niosomes to enhance antimicrobial activity against staphylococcus aureus strains |
| title_fullStr | Formulation, characterization and co-delivery of curcumin-rosemary loaded niosomes to enhance antimicrobial activity against staphylococcus aureus strains |
| title_full_unstemmed | Formulation, characterization and co-delivery of curcumin-rosemary loaded niosomes to enhance antimicrobial activity against staphylococcus aureus strains |
| title_short | Formulation, characterization and co-delivery of curcumin-rosemary loaded niosomes to enhance antimicrobial activity against staphylococcus aureus strains |
| title_sort | formulation characterization and co delivery of curcumin rosemary loaded niosomes to enhance antimicrobial activity against staphylococcus aureus strains |
| topic | antimicrobial curcumin niosome rosmarinus officinalis staphylococcus aureus |
| url | https://nmj.mums.ac.ir/article_25432_a8580205aec4fd19151912ee3f73d716.pdf |
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