Chlorpyrifos Detection Based on 9-Fluorenone Oxime
Chlorpyrifos is one of the most toxic organophosphate pesticides, prompting its ban in Europe in 2020. Consequently, developing a detection method that is both selective and sensitive is essential for protecting human health and the environment. In this study, we report for the first time a fluoresc...
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MDPI AG
2025-05-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/13/5/170 |
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| author | Edoardo Donà Aleksandra Lobnik |
| author_facet | Edoardo Donà Aleksandra Lobnik |
| author_sort | Edoardo Donà |
| collection | DOAJ |
| description | Chlorpyrifos is one of the most toxic organophosphate pesticides, prompting its ban in Europe in 2020. Consequently, developing a detection method that is both selective and sensitive is essential for protecting human health and the environment. In this study, we report for the first time a fluorescent probe based on an oxime for the direct detection of chlorpyrifos. 9-fluorenone oxime, upon deprotonation with a phosphazene base, undergoes a nucleophilic attack on chlorpyrifos, resulting in a significant alteration of its fluorescence properties. Following careful optimization, the method demonstrated excellent linearity (R<sup>2</sup> = 0.98) over a concentration range of 350 to 6980 μg/L, with a limit of detection of 15.5 μg/L. Furthermore, the probe was successfully applied to chlorpyrifos detection in water samples, yielding satisfactory results. This approach effectively overcomes the stability limitations of enzyme-based fluorescent sensors, offering a robust and innovative solution for the detection of organophosphate pesticides. |
| format | Article |
| id | doaj-art-c41fe798623143589fd59bd55be52154 |
| institution | DOAJ |
| issn | 2227-9040 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj-art-c41fe798623143589fd59bd55be521542025-08-20T03:14:32ZengMDPI AGChemosensors2227-90402025-05-0113517010.3390/chemosensors13050170Chlorpyrifos Detection Based on 9-Fluorenone OximeEdoardo Donà0Aleksandra Lobnik1Institute for Environmental Protection and Sensors, Beloruska 7, SI-2000 Maribor, SloveniaInstitute for Environmental Protection and Sensors, Beloruska 7, SI-2000 Maribor, SloveniaChlorpyrifos is one of the most toxic organophosphate pesticides, prompting its ban in Europe in 2020. Consequently, developing a detection method that is both selective and sensitive is essential for protecting human health and the environment. In this study, we report for the first time a fluorescent probe based on an oxime for the direct detection of chlorpyrifos. 9-fluorenone oxime, upon deprotonation with a phosphazene base, undergoes a nucleophilic attack on chlorpyrifos, resulting in a significant alteration of its fluorescence properties. Following careful optimization, the method demonstrated excellent linearity (R<sup>2</sup> = 0.98) over a concentration range of 350 to 6980 μg/L, with a limit of detection of 15.5 μg/L. Furthermore, the probe was successfully applied to chlorpyrifos detection in water samples, yielding satisfactory results. This approach effectively overcomes the stability limitations of enzyme-based fluorescent sensors, offering a robust and innovative solution for the detection of organophosphate pesticides.https://www.mdpi.com/2227-9040/13/5/170organophosphate pesticidechlorpyrifosfluorenone oximefluorescencephosphazene |
| spellingShingle | Edoardo Donà Aleksandra Lobnik Chlorpyrifos Detection Based on 9-Fluorenone Oxime Chemosensors organophosphate pesticide chlorpyrifos fluorenone oxime fluorescence phosphazene |
| title | Chlorpyrifos Detection Based on 9-Fluorenone Oxime |
| title_full | Chlorpyrifos Detection Based on 9-Fluorenone Oxime |
| title_fullStr | Chlorpyrifos Detection Based on 9-Fluorenone Oxime |
| title_full_unstemmed | Chlorpyrifos Detection Based on 9-Fluorenone Oxime |
| title_short | Chlorpyrifos Detection Based on 9-Fluorenone Oxime |
| title_sort | chlorpyrifos detection based on 9 fluorenone oxime |
| topic | organophosphate pesticide chlorpyrifos fluorenone oxime fluorescence phosphazene |
| url | https://www.mdpi.com/2227-9040/13/5/170 |
| work_keys_str_mv | AT edoardodona chlorpyrifosdetectionbasedon9fluorenoneoxime AT aleksandralobnik chlorpyrifosdetectionbasedon9fluorenoneoxime |