Generation of INS-jGCaMP7f knock-in Ca2+ reporter human embryonic stem cell line, GZLe001-C, using CRISPR/Cas9-based gene targeting

Generation of INS-jGCaMP7f knock-in Ca2+ reporter human embryonic stem cell line, GZLe001-C, using CRISPR/Cas9-based gene targeting

As a member of the single-fluorophore genetically encoded calcium indicators (GECIs), jGCaMP7f is widely applied to investigate intracellular Ca2+ concentrations. Here, we established an INS-jGCaMP7f knock-in H1 human embryonic stem cell (hESC) line by integrating jGCaMP7f gene into insulin locus vi...

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Bibliographic Details
Main Authors: Xin Liu, Feng Zhang, Deqi Chen, Jiaxiang Yin, Zirong Bi, Lihua Chen, Jiawei Yan, Qifei Dong, Wei Peng, Tao Xu, Yanying Guo, Haopeng Lin, Huisheng Liu
Format: Article
Language:English
Published: Elsevier 2025-02-01
Series:Stem Cell Research
Subjects:
jGCaMP7f
Ca2+
Insulin
hESC
CRISPR/Cas9
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506124003313
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