Impact of FokI (rs2228570) and BglI (rs739837) polymorphisms in VDR gene on permanent tooth eruption: A cross-sectional study
Introduction: Genetic polymorphisms who disturb the mineral homeostasis during tooth development and eruption are candidate to clarify the molecular mechanisms involved in changes in the tooth eruption chronology. In this study, we evaluate whether the FokI (rs2228570) and BglI (rs739837) polymorphi...
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Elsevier
2024-11-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426824001428 |
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| author | Caio Luiz Bitencourt Reis Kelem Cristina Cambraia Caproni Guerra Mariane Carolina Faria Barbosa Fabricio Fernandes Ferreira Daniela Coelho de Lima Raquel Assed Bezerra Segato Ana Cláudia Pedreira de Almeida Mirian Aiko Nakane Matsumoto Flares Baratto Filho Maria Angélica Hueb de Menezes Erika Calvano Küchler Daniela Silva Barroso de Oliveira |
| author_facet | Caio Luiz Bitencourt Reis Kelem Cristina Cambraia Caproni Guerra Mariane Carolina Faria Barbosa Fabricio Fernandes Ferreira Daniela Coelho de Lima Raquel Assed Bezerra Segato Ana Cláudia Pedreira de Almeida Mirian Aiko Nakane Matsumoto Flares Baratto Filho Maria Angélica Hueb de Menezes Erika Calvano Küchler Daniela Silva Barroso de Oliveira |
| author_sort | Caio Luiz Bitencourt Reis |
| collection | DOAJ |
| description | Introduction: Genetic polymorphisms who disturb the mineral homeostasis during tooth development and eruption are candidate to clarify the molecular mechanisms involved in changes in the tooth eruption chronology. In this study, we evaluate whether the FokI (rs2228570) and BglI (rs739837) polymorphisms in the Vitamin D receptor (VDR) gene are associated with changes in the chronology of eruption of permanent teeth. Material & method: This cross-sectional study randomly included 353 biologically unrelated children, both sexes, without systemic impairment or syndromes and history of trauma during the primary dentition. One operator perform the oral clinical examination. The tooth was considered erupted if there was a visible minimum of any tooth surface emerging from the mucosa. Genomic DNA was extracted from buccal epithelial cells from saliva samples. Genotyping was performed by Real-Time Polymerase Chain Reactions using TaqMan® technology. The average of the total number of erupted permanent teeth between the genotypes was compared by the Mann-Whitney test and multivariate Generalized Linear Models (GLM) (α = 5 %). β values with Confidence Interval (CI) 95 % were calculated. Results: The heterozygous adenine-guanine genotype of the FokI significantly decreases the number of erupted permanent teeth (β = −1.15; CI 95 % = −2.22 to −0.07; p = 0.036). In the stratified analysis for maxillary and mandibular teeth, this genotype was associated with a decrease in the number of erupted maxillary permanent teeth (β = −0.65; CI 95 % = −1.22 to −0.09; p = 0.023). BglI was not associated with permanent teeth eruption. Conclusion: The FokI, but not BglI, in the VDR may delay the eruption of permanent teeth. |
| format | Article |
| id | doaj-art-c40ffdcaa3aa47c5a84ea5aec7822695 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
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| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-c40ffdcaa3aa47c5a84ea5aec78226952024-11-27T05:02:13ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682024-11-01146700705Impact of FokI (rs2228570) and BglI (rs739837) polymorphisms in VDR gene on permanent tooth eruption: A cross-sectional studyCaio Luiz Bitencourt Reis0Kelem Cristina Cambraia Caproni Guerra1Mariane Carolina Faria Barbosa2Fabricio Fernandes Ferreira3Daniela Coelho de Lima4Raquel Assed Bezerra Segato5Ana Cláudia Pedreira de Almeida6Mirian Aiko Nakane Matsumoto7Flares Baratto Filho8Maria Angélica Hueb de Menezes9Erika Calvano Küchler10Daniela Silva Barroso de Oliveira11Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Brazil; Corresponding author. School of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café, s/n, Monte Alegre, Ribeirão Preto, São Paulo, 14040-904, Brazil.Department of Clinic and Surgery, School of Dentistry, Federal University of Alfenas, BrazilDepartment of Pediatric Dentistry, School of Dentistry, Federal University of Minas Gerais, BrazilDepartment of Clinic and Surgery, School of Dentistry, Federal University of Alfenas, BrazilDepartment of Clinic and Surgery, School of Dentistry, Federal University of Alfenas, BrazilDepartment of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, BrazilDepartment of Clinic and Surgery, School of Dentistry, Federal University of Alfenas, BrazilDepartment of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, BrazilSchool of Dentistry, Tuiuti University of Paraná, Brazil; School of Dentistry, Univille University, BrazilSchool of Dentistry, University de Uberaba, BrazilSchool of Dentistry, Tuiuti University of Paraná, Brazil; School of Dentistry, Univille University, BrazilDepartment of Clinic and Surgery, School of Dentistry, Federal University of Alfenas, BrazilIntroduction: Genetic polymorphisms who disturb the mineral homeostasis during tooth development and eruption are candidate to clarify the molecular mechanisms involved in changes in the tooth eruption chronology. In this study, we evaluate whether the FokI (rs2228570) and BglI (rs739837) polymorphisms in the Vitamin D receptor (VDR) gene are associated with changes in the chronology of eruption of permanent teeth. Material & method: This cross-sectional study randomly included 353 biologically unrelated children, both sexes, without systemic impairment or syndromes and history of trauma during the primary dentition. One operator perform the oral clinical examination. The tooth was considered erupted if there was a visible minimum of any tooth surface emerging from the mucosa. Genomic DNA was extracted from buccal epithelial cells from saliva samples. Genotyping was performed by Real-Time Polymerase Chain Reactions using TaqMan® technology. The average of the total number of erupted permanent teeth between the genotypes was compared by the Mann-Whitney test and multivariate Generalized Linear Models (GLM) (α = 5 %). β values with Confidence Interval (CI) 95 % were calculated. Results: The heterozygous adenine-guanine genotype of the FokI significantly decreases the number of erupted permanent teeth (β = −1.15; CI 95 % = −2.22 to −0.07; p = 0.036). In the stratified analysis for maxillary and mandibular teeth, this genotype was associated with a decrease in the number of erupted maxillary permanent teeth (β = −0.65; CI 95 % = −1.22 to −0.09; p = 0.023). BglI was not associated with permanent teeth eruption. Conclusion: The FokI, but not BglI, in the VDR may delay the eruption of permanent teeth.http://www.sciencedirect.com/science/article/pii/S2212426824001428Tooth eruptionSingle nucleotide polymorphismvdr |
| spellingShingle | Caio Luiz Bitencourt Reis Kelem Cristina Cambraia Caproni Guerra Mariane Carolina Faria Barbosa Fabricio Fernandes Ferreira Daniela Coelho de Lima Raquel Assed Bezerra Segato Ana Cláudia Pedreira de Almeida Mirian Aiko Nakane Matsumoto Flares Baratto Filho Maria Angélica Hueb de Menezes Erika Calvano Küchler Daniela Silva Barroso de Oliveira Impact of FokI (rs2228570) and BglI (rs739837) polymorphisms in VDR gene on permanent tooth eruption: A cross-sectional study Journal of Oral Biology and Craniofacial Research Tooth eruption Single nucleotide polymorphism vdr |
| title | Impact of FokI (rs2228570) and BglI (rs739837) polymorphisms in VDR gene on permanent tooth eruption: A cross-sectional study |
| title_full | Impact of FokI (rs2228570) and BglI (rs739837) polymorphisms in VDR gene on permanent tooth eruption: A cross-sectional study |
| title_fullStr | Impact of FokI (rs2228570) and BglI (rs739837) polymorphisms in VDR gene on permanent tooth eruption: A cross-sectional study |
| title_full_unstemmed | Impact of FokI (rs2228570) and BglI (rs739837) polymorphisms in VDR gene on permanent tooth eruption: A cross-sectional study |
| title_short | Impact of FokI (rs2228570) and BglI (rs739837) polymorphisms in VDR gene on permanent tooth eruption: A cross-sectional study |
| title_sort | impact of foki rs2228570 and bgli rs739837 polymorphisms in vdr gene on permanent tooth eruption a cross sectional study |
| topic | Tooth eruption Single nucleotide polymorphism vdr |
| url | http://www.sciencedirect.com/science/article/pii/S2212426824001428 |
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