Epidemiological characteristics of infectious bursal disease virus (IBDV) in China from 2023 to 2024: Mutated very virulent IBDV (mvvIBDV) is associated with atypical IBD

Infectious bursal disease (IBD), caused by the infectious bursal disease virus (IBDV), threatens the global poultry industry with acute immunosuppression. The non-lethal novel variant IBDV (nVarIBDV) has been identified as the primary cause of a new outbreak of atypical IBD in China. However, since...

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Main Authors: Hangbo Yu, Guodong Wang, Wenying Zhang, Ziwen Wu, Xinxin Niu, Mengmeng Huang, Yulong Zhang, Runhang Liu, Jinze Han, Mengmeng Xu, Jingzhe Han, Dan Ling, Erjing Ke, Suyan Wang, Hongyu Cui, Yanping Zhang, Yuntong Chen, Yongzhen Liu, Yulu Duan, Yulong Gao, Xiaole Qi
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:Poultry Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S0032579125004377
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author Hangbo Yu
Guodong Wang
Wenying Zhang
Ziwen Wu
Xinxin Niu
Mengmeng Huang
Yulong Zhang
Runhang Liu
Jinze Han
Mengmeng Xu
Jingzhe Han
Dan Ling
Erjing Ke
Suyan Wang
Hongyu Cui
Yanping Zhang
Yuntong Chen
Yongzhen Liu
Yulu Duan
Yulong Gao
Xiaole Qi
author_facet Hangbo Yu
Guodong Wang
Wenying Zhang
Ziwen Wu
Xinxin Niu
Mengmeng Huang
Yulong Zhang
Runhang Liu
Jinze Han
Mengmeng Xu
Jingzhe Han
Dan Ling
Erjing Ke
Suyan Wang
Hongyu Cui
Yanping Zhang
Yuntong Chen
Yongzhen Liu
Yulu Duan
Yulong Gao
Xiaole Qi
author_sort Hangbo Yu
collection DOAJ
description Infectious bursal disease (IBD), caused by the infectious bursal disease virus (IBDV), threatens the global poultry industry with acute immunosuppression. The non-lethal novel variant IBDV (nVarIBDV) has been identified as the primary cause of a new outbreak of atypical IBD in China. However, since 2023, several poultry farms in China have continuously reported the detection of vvIBDV in chicken flocks exhibiting atypical IBD characteristics. To analyze this abnormal problem, the prevalence of IBDV in major poultry farming areas in China from 2023 to 2024 was monitored. Our epidemiological survey revealed four coexisting IBDV strains in China, nVarIBDV (69.9 %, 65/93) ranked the first, followed by vvIBDV (16.1 %, 15/93), attIBDV (8.6 %, 8/93), and cIBDV (5.4 %, 5/93). Notably, all these 15 vvIBDV strains detected in this study harbored single or combination mutations in the VP2 hypervariable region (HVR) including residue 279: D279N (seven strains), G254D/I256L/D279N (one strain), or A222T/G254D/I256L/D279N (seven strains). Furthermore, one representative strain of IBDV-NMG23-1701 which contained the D279N mutation was isolated. In the phylogenetic tree constructed based on the IBDV genome, IBDV-NMG23-1701 was located in the vvIBDV branch. However, IBDV-NMG23-1701 did not kill chickens directly but induced severe subclinical symptoms of atypical IBD. We named these strains mutated vvIBDV (mvvIBDV), which genotypically belonged to vvIBDV but exhibited atypical IBD symptoms. The mvvIBDV has been detected in several major poultry farming areas in China. This is the first study to identify mvvIBDV as a new pathogen causing atypical IBD, providing a scientific basis for optimizing prevention and control strategies.
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spelling doaj-art-c40cd2771af94135b6822da039bc7d3b2025-08-20T02:40:10ZengElsevierPoultry Science0032-57912025-07-01104710519510.1016/j.psj.2025.105195Epidemiological characteristics of infectious bursal disease virus (IBDV) in China from 2023 to 2024: Mutated very virulent IBDV (mvvIBDV) is associated with atypical IBDHangbo Yu0Guodong Wang1Wenying Zhang2Ziwen Wu3Xinxin Niu4Mengmeng Huang5Yulong Zhang6Runhang Liu7Jinze Han8Mengmeng Xu9Jingzhe Han10Dan Ling11Erjing Ke12Suyan Wang13Hongyu Cui14Yanping Zhang15Yuntong Chen16Yongzhen Liu17Yulu Duan18Yulong Gao19Xiaole Qi20Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, 225009, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, 225009, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, 225009, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, ChinaAvian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, 225009, China; Corresponding authors at: Avian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, No. 678 Haping Road, Xiangfang District, Harbin 150069, Heilongjiang Province, PR China.Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; WOAH Reference Laboratory for Infectious Bursal Disease, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin, 150069, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou, 225009, China; Corresponding authors at: Avian Immunosuppressive Diseases Division, State Key Laboratory of Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, No. 678 Haping Road, Xiangfang District, Harbin 150069, Heilongjiang Province, PR China.Infectious bursal disease (IBD), caused by the infectious bursal disease virus (IBDV), threatens the global poultry industry with acute immunosuppression. The non-lethal novel variant IBDV (nVarIBDV) has been identified as the primary cause of a new outbreak of atypical IBD in China. However, since 2023, several poultry farms in China have continuously reported the detection of vvIBDV in chicken flocks exhibiting atypical IBD characteristics. To analyze this abnormal problem, the prevalence of IBDV in major poultry farming areas in China from 2023 to 2024 was monitored. Our epidemiological survey revealed four coexisting IBDV strains in China, nVarIBDV (69.9 %, 65/93) ranked the first, followed by vvIBDV (16.1 %, 15/93), attIBDV (8.6 %, 8/93), and cIBDV (5.4 %, 5/93). Notably, all these 15 vvIBDV strains detected in this study harbored single or combination mutations in the VP2 hypervariable region (HVR) including residue 279: D279N (seven strains), G254D/I256L/D279N (one strain), or A222T/G254D/I256L/D279N (seven strains). Furthermore, one representative strain of IBDV-NMG23-1701 which contained the D279N mutation was isolated. In the phylogenetic tree constructed based on the IBDV genome, IBDV-NMG23-1701 was located in the vvIBDV branch. However, IBDV-NMG23-1701 did not kill chickens directly but induced severe subclinical symptoms of atypical IBD. We named these strains mutated vvIBDV (mvvIBDV), which genotypically belonged to vvIBDV but exhibited atypical IBD symptoms. The mvvIBDV has been detected in several major poultry farming areas in China. This is the first study to identify mvvIBDV as a new pathogen causing atypical IBD, providing a scientific basis for optimizing prevention and control strategies.http://www.sciencedirect.com/science/article/pii/S0032579125004377Atypical IBDNovel variant IBDV (nVarIBDV)Mutated very virulent IBDV (mvvIBDV)PathogenicitySubclinical symptoms
spellingShingle Hangbo Yu
Guodong Wang
Wenying Zhang
Ziwen Wu
Xinxin Niu
Mengmeng Huang
Yulong Zhang
Runhang Liu
Jinze Han
Mengmeng Xu
Jingzhe Han
Dan Ling
Erjing Ke
Suyan Wang
Hongyu Cui
Yanping Zhang
Yuntong Chen
Yongzhen Liu
Yulu Duan
Yulong Gao
Xiaole Qi
Epidemiological characteristics of infectious bursal disease virus (IBDV) in China from 2023 to 2024: Mutated very virulent IBDV (mvvIBDV) is associated with atypical IBD
Poultry Science
Atypical IBD
Novel variant IBDV (nVarIBDV)
Mutated very virulent IBDV (mvvIBDV)
Pathogenicity
Subclinical symptoms
title Epidemiological characteristics of infectious bursal disease virus (IBDV) in China from 2023 to 2024: Mutated very virulent IBDV (mvvIBDV) is associated with atypical IBD
title_full Epidemiological characteristics of infectious bursal disease virus (IBDV) in China from 2023 to 2024: Mutated very virulent IBDV (mvvIBDV) is associated with atypical IBD
title_fullStr Epidemiological characteristics of infectious bursal disease virus (IBDV) in China from 2023 to 2024: Mutated very virulent IBDV (mvvIBDV) is associated with atypical IBD
title_full_unstemmed Epidemiological characteristics of infectious bursal disease virus (IBDV) in China from 2023 to 2024: Mutated very virulent IBDV (mvvIBDV) is associated with atypical IBD
title_short Epidemiological characteristics of infectious bursal disease virus (IBDV) in China from 2023 to 2024: Mutated very virulent IBDV (mvvIBDV) is associated with atypical IBD
title_sort epidemiological characteristics of infectious bursal disease virus ibdv in china from 2023 to 2024 mutated very virulent ibdv mvvibdv is associated with atypical ibd
topic Atypical IBD
Novel variant IBDV (nVarIBDV)
Mutated very virulent IBDV (mvvIBDV)
Pathogenicity
Subclinical symptoms
url http://www.sciencedirect.com/science/article/pii/S0032579125004377
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