Assembly and comparative analysis of the complete mitochondrial genome of Abies beshanzuensis: insights into conservation genomics of a critically endangered fir

Abstract Background Abies beshanzuensis, an endemic fir native to the subtropical high mountains of eastern China, is classified as a first-class nationally protected wild plant and is listed as Critically Endangered (CR) by the International Union for Conservation of Nature (IUCN). Taxonomic contro...

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Main Authors: Xingjian Liu, Shiyu Hu, Jiahao Shen, Mimi Li
Format: Article
Language:English
Published: BMC 2025-08-01
Series:BMC Plant Biology
Subjects:
Online Access:https://doi.org/10.1186/s12870-025-07132-2
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author Xingjian Liu
Shiyu Hu
Jiahao Shen
Mimi Li
author_facet Xingjian Liu
Shiyu Hu
Jiahao Shen
Mimi Li
author_sort Xingjian Liu
collection DOAJ
description Abstract Background Abies beshanzuensis, an endemic fir native to the subtropical high mountains of eastern China, is classified as a first-class nationally protected wild plant and is listed as Critically Endangered (CR) by the International Union for Conservation of Nature (IUCN). Taxonomic controversies have long persisted among A. beshanzuensis, A. ziyuanensis, and A. dayuanensis, yet studies focusing on their mitochondrial DNA remain limited. Results In this study, the complete mitochondrial genome of A. beshanzuensis was assembled using a combination of Oxford Nanopore long-read sequencing and Illumina short-read sequencing technologies. The mitochondrial genome is 1.06 Mb in size with a GC content of 45.97%, and consists of nine linear chromosomes and one circular chromosome. Comprehensive analyses, including genome annotation, structural comparisons, RNA editing site prediction, and phylogenetic reconstruction, were subsequently conducted. Conclusion The results provide important insights into the mitochondrial genome architecture of A. beshanzuensis, offering a valuable resource for clarifying its controversial taxonomic status and advancing the understanding of evolutionary relationships within the genus Abies.
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publishDate 2025-08-01
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series BMC Plant Biology
spelling doaj-art-c3f42773616346f2bd02755a9e1b4cd52025-08-20T03:42:40ZengBMCBMC Plant Biology1471-22292025-08-0125111310.1186/s12870-025-07132-2Assembly and comparative analysis of the complete mitochondrial genome of Abies beshanzuensis: insights into conservation genomics of a critically endangered firXingjian Liu0Shiyu Hu1Jiahao Shen2Mimi Li3Institute of Botany, Jiangsu Province and Chinese Academy of SciencesInstitute of Botany, Jiangsu Province and Chinese Academy of SciencesInstitute of Botany, Jiangsu Province and Chinese Academy of SciencesInstitute of Botany, Jiangsu Province and Chinese Academy of SciencesAbstract Background Abies beshanzuensis, an endemic fir native to the subtropical high mountains of eastern China, is classified as a first-class nationally protected wild plant and is listed as Critically Endangered (CR) by the International Union for Conservation of Nature (IUCN). Taxonomic controversies have long persisted among A. beshanzuensis, A. ziyuanensis, and A. dayuanensis, yet studies focusing on their mitochondrial DNA remain limited. Results In this study, the complete mitochondrial genome of A. beshanzuensis was assembled using a combination of Oxford Nanopore long-read sequencing and Illumina short-read sequencing technologies. The mitochondrial genome is 1.06 Mb in size with a GC content of 45.97%, and consists of nine linear chromosomes and one circular chromosome. Comprehensive analyses, including genome annotation, structural comparisons, RNA editing site prediction, and phylogenetic reconstruction, were subsequently conducted. Conclusion The results provide important insights into the mitochondrial genome architecture of A. beshanzuensis, offering a valuable resource for clarifying its controversial taxonomic status and advancing the understanding of evolutionary relationships within the genus Abies.https://doi.org/10.1186/s12870-025-07132-2Abies beshanzuensisFirMitochondrial genomePhylogeny
spellingShingle Xingjian Liu
Shiyu Hu
Jiahao Shen
Mimi Li
Assembly and comparative analysis of the complete mitochondrial genome of Abies beshanzuensis: insights into conservation genomics of a critically endangered fir
BMC Plant Biology
Abies beshanzuensis
Fir
Mitochondrial genome
Phylogeny
title Assembly and comparative analysis of the complete mitochondrial genome of Abies beshanzuensis: insights into conservation genomics of a critically endangered fir
title_full Assembly and comparative analysis of the complete mitochondrial genome of Abies beshanzuensis: insights into conservation genomics of a critically endangered fir
title_fullStr Assembly and comparative analysis of the complete mitochondrial genome of Abies beshanzuensis: insights into conservation genomics of a critically endangered fir
title_full_unstemmed Assembly and comparative analysis of the complete mitochondrial genome of Abies beshanzuensis: insights into conservation genomics of a critically endangered fir
title_short Assembly and comparative analysis of the complete mitochondrial genome of Abies beshanzuensis: insights into conservation genomics of a critically endangered fir
title_sort assembly and comparative analysis of the complete mitochondrial genome of abies beshanzuensis insights into conservation genomics of a critically endangered fir
topic Abies beshanzuensis
Fir
Mitochondrial genome
Phylogeny
url https://doi.org/10.1186/s12870-025-07132-2
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AT jiahaoshen assemblyandcomparativeanalysisofthecompletemitochondrialgenomeofabiesbeshanzuensisinsightsintoconservationgenomicsofacriticallyendangeredfir
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