ARF4 acting upstream of LBD16 promotes adventitious root formation in peach
Although class A auxin response factors (ARFs) are known to regulate adventitious root (AR) development through the canonical SCFTIR1-Aux/IAA-ARF signaling pathway, the regulatory role of class B ARFs in AR development remains largely unclear. Therefore, this research focused on the role of class B...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2025-01-01
|
| Series: | Horticultural Plant Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014123001735 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850101199075803136 |
|---|---|
| author | Jingjing Liu Lingling Gao Ruoxi Zhang Anqi Gao Zamith Kerubo Oginga Beibei Zheng Yuepeng Han |
| author_facet | Jingjing Liu Lingling Gao Ruoxi Zhang Anqi Gao Zamith Kerubo Oginga Beibei Zheng Yuepeng Han |
| author_sort | Jingjing Liu |
| collection | DOAJ |
| description | Although class A auxin response factors (ARFs) are known to regulate adventitious root (AR) development through the canonical SCFTIR1-Aux/IAA-ARF signaling pathway, the regulatory role of class B ARFs in AR development remains largely unclear. Therefore, this research focused on the role of class B ARF transcription factors in peach (Prunus persica ‘Shengli’) adventitious root formation. Here, we report the role of a class B ARF gene PpARF4 in adventitious root formation in peach. Comparative transcriptome and qRT-PCR analyses showed that the transcription of PpARF4 was significantly up-regulated in auxin-treated stem explants. Y2H assay showed that PpARF4 had no interaction with PpIAAs (AUXIN/INDOLE ACETIC ACIDs). PpARF4 could bind the promoters of lateral root development gene PpLBD16 and auxin transport gene PpPIN1 to activate their transcription. Ectopic overexpression of PpARF4 and PpLBD16 in Arabidopsis promoted AR development. Additionally, PpARF4 could act as a negative regulator of flavone synthesis and thus prevent the explants from browning. The results not only provide novel insights into the functions of ARFs in regulating plant growth and development, but will also be useful for fulfilling asexual propagation by stem cuttings in peach. |
| format | Article |
| id | doaj-art-c3f2a5f26deb4a98a92b31017e4c50dc |
| institution | DOAJ |
| issn | 2468-0141 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj-art-c3f2a5f26deb4a98a92b31017e4c50dc2025-08-20T02:40:06ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412025-01-0111114516110.1016/j.hpj.2023.07.004ARF4 acting upstream of LBD16 promotes adventitious root formation in peachJingjing Liu0Lingling Gao1Ruoxi Zhang2Anqi Gao3Zamith Kerubo Oginga4Beibei Zheng5Yuepeng Han6CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design of Chinese Academy of Sciences, Wuhan, Hubei 430074, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design of Chinese Academy of Sciences, Wuhan, Hubei 430074, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design of Chinese Academy of Sciences, Wuhan, Hubei 430074, China; Hubei Hongshan Laboratory, Wuhan, Hubei 430070, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design of Chinese Academy of Sciences, Wuhan, Hubei 430074, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design of Chinese Academy of Sciences, Wuhan, Hubei 430074, ChinaCAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design of Chinese Academy of Sciences, Wuhan, Hubei 430074, China; Hubei Hongshan Laboratory, Wuhan, Hubei 430070, China; Corresponding authors.CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Innovative Academy of Seed Design of Chinese Academy of Sciences, Wuhan, Hubei 430074, China; Hubei Hongshan Laboratory, Wuhan, Hubei 430070, China; Corresponding authors.Although class A auxin response factors (ARFs) are known to regulate adventitious root (AR) development through the canonical SCFTIR1-Aux/IAA-ARF signaling pathway, the regulatory role of class B ARFs in AR development remains largely unclear. Therefore, this research focused on the role of class B ARF transcription factors in peach (Prunus persica ‘Shengli’) adventitious root formation. Here, we report the role of a class B ARF gene PpARF4 in adventitious root formation in peach. Comparative transcriptome and qRT-PCR analyses showed that the transcription of PpARF4 was significantly up-regulated in auxin-treated stem explants. Y2H assay showed that PpARF4 had no interaction with PpIAAs (AUXIN/INDOLE ACETIC ACIDs). PpARF4 could bind the promoters of lateral root development gene PpLBD16 and auxin transport gene PpPIN1 to activate their transcription. Ectopic overexpression of PpARF4 and PpLBD16 in Arabidopsis promoted AR development. Additionally, PpARF4 could act as a negative regulator of flavone synthesis and thus prevent the explants from browning. The results not only provide novel insights into the functions of ARFs in regulating plant growth and development, but will also be useful for fulfilling asexual propagation by stem cuttings in peach.http://www.sciencedirect.com/science/article/pii/S2468014123001735Prunus persicaAdventitious rootAuxinPpARF4PpLBD16 |
| spellingShingle | Jingjing Liu Lingling Gao Ruoxi Zhang Anqi Gao Zamith Kerubo Oginga Beibei Zheng Yuepeng Han ARF4 acting upstream of LBD16 promotes adventitious root formation in peach Horticultural Plant Journal Prunus persica Adventitious root Auxin PpARF4 PpLBD16 |
| title | ARF4 acting upstream of LBD16 promotes adventitious root formation in peach |
| title_full | ARF4 acting upstream of LBD16 promotes adventitious root formation in peach |
| title_fullStr | ARF4 acting upstream of LBD16 promotes adventitious root formation in peach |
| title_full_unstemmed | ARF4 acting upstream of LBD16 promotes adventitious root formation in peach |
| title_short | ARF4 acting upstream of LBD16 promotes adventitious root formation in peach |
| title_sort | arf4 acting upstream of lbd16 promotes adventitious root formation in peach |
| topic | Prunus persica Adventitious root Auxin PpARF4 PpLBD16 |
| url | http://www.sciencedirect.com/science/article/pii/S2468014123001735 |
| work_keys_str_mv | AT jingjingliu arf4actingupstreamoflbd16promotesadventitiousrootformationinpeach AT linglinggao arf4actingupstreamoflbd16promotesadventitiousrootformationinpeach AT ruoxizhang arf4actingupstreamoflbd16promotesadventitiousrootformationinpeach AT anqigao arf4actingupstreamoflbd16promotesadventitiousrootformationinpeach AT zamithkerubooginga arf4actingupstreamoflbd16promotesadventitiousrootformationinpeach AT beibeizheng arf4actingupstreamoflbd16promotesadventitiousrootformationinpeach AT yuepenghan arf4actingupstreamoflbd16promotesadventitiousrootformationinpeach |