Construction of the recombinant Listeria monocytogenes mutant expressing green fluorescent protein
A recombinant Listeria monocyogenes mutant expressing green fluorescent protein (GFP) gene was constructed by homologous recombination. The target gene gfp was fused to the actA gene fragment downstream of its promoter and signal sequence. PCR amplification and restriction digestion of the PCR produ...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2005-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2005.05.0638 |
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| author | KE Chun-lin SONG Hou-hui JIANG Ling-li ZHANG Gui-zhi FANG Wei-huan |
| author_facet | KE Chun-lin SONG Hou-hui JIANG Ling-li ZHANG Gui-zhi FANG Wei-huan |
| author_sort | KE Chun-lin |
| collection | DOAJ |
| description | A recombinant Listeria monocyogenes mutant expressing green fluorescent protein (GFP) gene was constructed by homologous recombination. The target gene gfp was fused to the actA gene fragment downstream of its promoter and signal sequence. PCR amplification and restriction digestion of the PCR product confirmed the insertional fusion. The reporter gene was expressed as shown by fluorescent microscopy and SDS-PAGE/western blotting. The recombinant strain exhibited reduced invasiveness to the in vitro cultured cells as well as decreased virulence to the chicken embryos and mice. The strain has potential applications in microbe-host interaction studies in the medical or veterinary medical fields and in food microbiological studies. |
| format | Article |
| id | doaj-art-c3f087f3f0944d3e96ffcffe96d301e2 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2005-09-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-c3f087f3f0944d3e96ffcffe96d301e22025-08-20T03:16:04ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552005-09-013163864410.3785/1008-9209.2005.05.063810089209Construction of the recombinant Listeria monocytogenes mutant expressing green fluorescent proteinKE Chun-linSONG Hou-huiJIANG Ling-liZHANG Gui-zhiFANG Wei-huanA recombinant Listeria monocyogenes mutant expressing green fluorescent protein (GFP) gene was constructed by homologous recombination. The target gene gfp was fused to the actA gene fragment downstream of its promoter and signal sequence. PCR amplification and restriction digestion of the PCR product confirmed the insertional fusion. The reporter gene was expressed as shown by fluorescent microscopy and SDS-PAGE/western blotting. The recombinant strain exhibited reduced invasiveness to the in vitro cultured cells as well as decreased virulence to the chicken embryos and mice. The strain has potential applications in microbe-host interaction studies in the medical or veterinary medical fields and in food microbiological studies.https://www.academax.com/doi/10.3785/1008-9209.2005.05.0638<italic>Listeria monocytogenes</italic>homologous recombinationgreen fluorescent proteinheterologous gene expression |
| spellingShingle | KE Chun-lin SONG Hou-hui JIANG Ling-li ZHANG Gui-zhi FANG Wei-huan Construction of the recombinant Listeria monocytogenes mutant expressing green fluorescent protein 浙江大学学报. 农业与生命科学版 <italic>Listeria monocytogenes</italic> homologous recombination green fluorescent protein heterologous gene expression |
| title | Construction of the recombinant Listeria monocytogenes mutant expressing green fluorescent protein |
| title_full | Construction of the recombinant Listeria monocytogenes mutant expressing green fluorescent protein |
| title_fullStr | Construction of the recombinant Listeria monocytogenes mutant expressing green fluorescent protein |
| title_full_unstemmed | Construction of the recombinant Listeria monocytogenes mutant expressing green fluorescent protein |
| title_short | Construction of the recombinant Listeria monocytogenes mutant expressing green fluorescent protein |
| title_sort | construction of the recombinant listeria monocytogenes mutant expressing green fluorescent protein |
| topic | <italic>Listeria monocytogenes</italic> homologous recombination green fluorescent protein heterologous gene expression |
| url | https://www.academax.com/doi/10.3785/1008-9209.2005.05.0638 |
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