Conductive Composite Biosensor System for Electrochemical Indinavir Drug Detection
Indinavir is a protease inhibitor antiretroviral (ARV) drug, which forms part of the highly active antiretroviral therapy during the treatment of HIV/AIDS. Indinavir undergoes first-pass metabolism through the cytochrome P450 (CYP) enzymes in the human liver, of which CYP3A4 is the most influential...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/630408 |
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| author | Natasha Ross Nicolette Hendricks-Leukes Rachel Fanelwa Ajayi Priscilla Baker Emmanuel I. Iwuoha |
| author_facet | Natasha Ross Nicolette Hendricks-Leukes Rachel Fanelwa Ajayi Priscilla Baker Emmanuel I. Iwuoha |
| author_sort | Natasha Ross |
| collection | DOAJ |
| description | Indinavir is a protease inhibitor antiretroviral (ARV) drug, which forms part of the highly active antiretroviral therapy during the treatment of HIV/AIDS. Indinavir undergoes first-pass metabolism through the cytochrome P450 (CYP) enzymes in the human liver, of which CYP3A4 is the most influential isoenzyme. Multidrug combination therapy and, as such, therapeutic drug monitoring (TDM) during HIV/AIDS treatment are therefore critical, to prevent adverse interactions. The conventional sensitive and specific assays available for quantifying ARV drugs, however, suffer from distinct disadvantages. In this regard, biosensors can be used to provide real time information on the metabolic profile of the drug. In this study, a biosensor with cobalt(III) sepulchrate trichloride {CoSep3+} as diffusional mediator was constructed. The biosensor platform consisted of CYP3A4 immobilized onto a gold nanoparticle (GNP) overoxidized polypyrrole (OvOxPpy) carrier matrix. The biosensor exhibited reversible electrochemistry, with formal potential determined as −624 ± 5 mV, from voltammetric analysis, with overall electron transfer being diffusion controlled. The biosensor showed typical electrocatalytic response to dioxygen (O2), exemplified by the distinct increase in the cathodic peak current (Ip,c). A concentration-dependent increase in Ip,c was observed in response to consecutive additions of Indinavir. |
| format | Article |
| id | doaj-art-c3ea64bc8fed4c08aa10ead420766d0b |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-c3ea64bc8fed4c08aa10ead420766d0b2025-02-03T01:10:43ZengWileyJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/630408630408Conductive Composite Biosensor System for Electrochemical Indinavir Drug DetectionNatasha Ross0Nicolette Hendricks-Leukes1Rachel Fanelwa Ajayi2Priscilla Baker3Emmanuel I. Iwuoha4SensorLab, Department of Chemistry, University of Western Cape, Private Bag X17, Bellville 7535, South AfricaSensorLab, Department of Chemistry, University of Western Cape, Private Bag X17, Bellville 7535, South AfricaSensorLab, Department of Chemistry, University of Western Cape, Private Bag X17, Bellville 7535, South AfricaSensorLab, Department of Chemistry, University of Western Cape, Private Bag X17, Bellville 7535, South AfricaSensorLab, Department of Chemistry, University of Western Cape, Private Bag X17, Bellville 7535, South AfricaIndinavir is a protease inhibitor antiretroviral (ARV) drug, which forms part of the highly active antiretroviral therapy during the treatment of HIV/AIDS. Indinavir undergoes first-pass metabolism through the cytochrome P450 (CYP) enzymes in the human liver, of which CYP3A4 is the most influential isoenzyme. Multidrug combination therapy and, as such, therapeutic drug monitoring (TDM) during HIV/AIDS treatment are therefore critical, to prevent adverse interactions. The conventional sensitive and specific assays available for quantifying ARV drugs, however, suffer from distinct disadvantages. In this regard, biosensors can be used to provide real time information on the metabolic profile of the drug. In this study, a biosensor with cobalt(III) sepulchrate trichloride {CoSep3+} as diffusional mediator was constructed. The biosensor platform consisted of CYP3A4 immobilized onto a gold nanoparticle (GNP) overoxidized polypyrrole (OvOxPpy) carrier matrix. The biosensor exhibited reversible electrochemistry, with formal potential determined as −624 ± 5 mV, from voltammetric analysis, with overall electron transfer being diffusion controlled. The biosensor showed typical electrocatalytic response to dioxygen (O2), exemplified by the distinct increase in the cathodic peak current (Ip,c). A concentration-dependent increase in Ip,c was observed in response to consecutive additions of Indinavir.http://dx.doi.org/10.1155/2015/630408 |
| spellingShingle | Natasha Ross Nicolette Hendricks-Leukes Rachel Fanelwa Ajayi Priscilla Baker Emmanuel I. Iwuoha Conductive Composite Biosensor System for Electrochemical Indinavir Drug Detection Journal of Chemistry |
| title | Conductive Composite Biosensor System for Electrochemical Indinavir Drug Detection |
| title_full | Conductive Composite Biosensor System for Electrochemical Indinavir Drug Detection |
| title_fullStr | Conductive Composite Biosensor System for Electrochemical Indinavir Drug Detection |
| title_full_unstemmed | Conductive Composite Biosensor System for Electrochemical Indinavir Drug Detection |
| title_short | Conductive Composite Biosensor System for Electrochemical Indinavir Drug Detection |
| title_sort | conductive composite biosensor system for electrochemical indinavir drug detection |
| url | http://dx.doi.org/10.1155/2015/630408 |
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