Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm Genotypes

Cinnamomum tamala Nees & Eberm. is an important traditional medicinal plant, mentioned in various ancient literatures such as Ayurveda. Several of its medicinal properties have recently been proved. To characterize diversity in terms of metabolite profiles of Cinnamomum tamala Nees and Eberm g...

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Main Authors: Vineeta Singh, Atul Kumar Gupta, S. P. Singh, Anil Kumar
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/2012/549265
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author Vineeta Singh
Atul Kumar Gupta
S. P. Singh
Anil Kumar
author_facet Vineeta Singh
Atul Kumar Gupta
S. P. Singh
Anil Kumar
author_sort Vineeta Singh
collection DOAJ
description Cinnamomum tamala Nees & Eberm. is an important traditional medicinal plant, mentioned in various ancient literatures such as Ayurveda. Several of its medicinal properties have recently been proved. To characterize diversity in terms of metabolite profiles of Cinnamomum tamala Nees and Eberm genotypes, a newly emerging mass spectral ionization technique direct time in real time (DART) is very helpful. The DART ion source has been used to analyze an extremely wide range of phytochemicals present in leaves of Cinnamomum tamala. Ten genotypes were assessed for the presence of different phytochemicals. Phytochemical analysis showed the presence of mainly terpenes and phenols. These constituents vary in the different genotypes of Cinnamomum tamala. Principal component analysis has also been employed to analyze the DART data of these Cinnamomum genotypes. The result shows that the genotype of Cinnamomum tamala could be differentiated using DART MS data. The active components present in Cinnamomum tamala may be contributing significantly to high amount of antioxidant property of leaves and, in turn, conditional effects for diabetic patients.
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spelling doaj-art-c3e75180b18543308062f90d4070cd702025-02-03T01:22:48ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/549265549265Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm GenotypesVineeta Singh0Atul Kumar Gupta1S. P. Singh2Anil Kumar3Department of Molecular Biology & Genetic Engineering, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Uttarakhand, Pantnagar 263145, IndiaDepartment of Molecular Biology & Genetic Engineering, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Uttarakhand, Pantnagar 263145, IndiaDepartment of Pharmacology & Toxicology, College of Veterinary Science, G. B. Pant University of Agriculture and Technology, Uttarakhand, Pantnagar 263145, IndiaDepartment of Molecular Biology & Genetic Engineering, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Uttarakhand, Pantnagar 263145, IndiaCinnamomum tamala Nees & Eberm. is an important traditional medicinal plant, mentioned in various ancient literatures such as Ayurveda. Several of its medicinal properties have recently been proved. To characterize diversity in terms of metabolite profiles of Cinnamomum tamala Nees and Eberm genotypes, a newly emerging mass spectral ionization technique direct time in real time (DART) is very helpful. The DART ion source has been used to analyze an extremely wide range of phytochemicals present in leaves of Cinnamomum tamala. Ten genotypes were assessed for the presence of different phytochemicals. Phytochemical analysis showed the presence of mainly terpenes and phenols. These constituents vary in the different genotypes of Cinnamomum tamala. Principal component analysis has also been employed to analyze the DART data of these Cinnamomum genotypes. The result shows that the genotype of Cinnamomum tamala could be differentiated using DART MS data. The active components present in Cinnamomum tamala may be contributing significantly to high amount of antioxidant property of leaves and, in turn, conditional effects for diabetic patients.http://dx.doi.org/10.1100/2012/549265
spellingShingle Vineeta Singh
Atul Kumar Gupta
S. P. Singh
Anil Kumar
Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm Genotypes
The Scientific World Journal
title Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm Genotypes
title_full Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm Genotypes
title_fullStr Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm Genotypes
title_full_unstemmed Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm Genotypes
title_short Direct Analysis in Real Time by Mass Spectrometric Technique for Determining the Variation in Metabolite Profiles of Cinnamomum tamala Nees and Eberm Genotypes
title_sort direct analysis in real time by mass spectrometric technique for determining the variation in metabolite profiles of cinnamomum tamala nees and eberm genotypes
url http://dx.doi.org/10.1100/2012/549265
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