Carboxymethylation of the polysaccharide from the fermentation broth of Marasmius androsaceus and its antidepressant mechanisms
To investigate the structure-activity relationship of polysaccharide and obtain a better antidepressant polysaccharide, the antidepressant-like activity of a carboxymethyl polysaccharide (C-MEPS2) subjected to submerged fermentation was systematically studied. PC12-H cell and Kunming mice were used...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000617 |
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| author | Yu Qiu Yan Su Jia Song Fangming Mou Jia Gou Xiaoqi Geng Xinyue Li Zhiqiang Nie Jianxin Wang Yu Zheng Min Wang |
| author_facet | Yu Qiu Yan Su Jia Song Fangming Mou Jia Gou Xiaoqi Geng Xinyue Li Zhiqiang Nie Jianxin Wang Yu Zheng Min Wang |
| author_sort | Yu Qiu |
| collection | DOAJ |
| description | To investigate the structure-activity relationship of polysaccharide and obtain a better antidepressant polysaccharide, the antidepressant-like activity of a carboxymethyl polysaccharide (C-MEPS2) subjected to submerged fermentation was systematically studied. PC12-H cell and Kunming mice were used to investigate the differences and their mechanism in the antidepressant effects of C-MEPS2 and MEPS2. Cell experiments have showed that C-MEPS2 has a better antidepressant effect than MEPS2. C-MEPS2 could exert antidepressant effects related to catecholamine synthesis with specific sites of TH, D2DR, and P-CAMKII. In addition, C-MEPS2 could repair the Res-induced damage in PC12-H cell, stabilize the mitochondrial membrane potential and regulate intracellular Ca2+ concentration, thus reducing cell apoptosis caused by RES. Antagonists common dosing experiments on animals further proved that CMEPS2 could significantly improve the antidepressant effect of derivatives without affecting the antidepressant mechanism of MEPS2. It is speculated that it may be related to carboxymethylated modification. |
| format | Article |
| id | doaj-art-c3e6f4b997c84ee9a8ed43173a1cb4f8 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-c3e6f4b997c84ee9a8ed43173a1cb4f82025-02-03T02:42:14ZengTsinghua University PressFood Science and Human Wellness2213-45302023-11-0112624172427Carboxymethylation of the polysaccharide from the fermentation broth of Marasmius androsaceus and its antidepressant mechanismsYu Qiu0Yan Su1Jia Song2Fangming Mou3Jia Gou4Xiaoqi Geng5Xinyue Li6Zhiqiang Nie7Jianxin Wang8Yu Zheng9Min Wang10State Key Laboratory of Food Nutrition and Safety, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China; Corresponding author at: State Key Laboratory of Food Nutrition and Safety, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, ChinaKey Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, ChinaWisconsin Center for NanoBioSystems, School of Pharmacy, University of Wisconsin-Madison, Madison, WI 53706, USA; Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, Madison, WI 53706, USAState Key Laboratory of Food Nutrition and Safety, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China; Corresponding author at: State Key Laboratory of Food Nutrition and Safety, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China; Corresponding author at: State Key Laboratory of Food Nutrition and Safety, Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, ChinaTo investigate the structure-activity relationship of polysaccharide and obtain a better antidepressant polysaccharide, the antidepressant-like activity of a carboxymethyl polysaccharide (C-MEPS2) subjected to submerged fermentation was systematically studied. PC12-H cell and Kunming mice were used to investigate the differences and their mechanism in the antidepressant effects of C-MEPS2 and MEPS2. Cell experiments have showed that C-MEPS2 has a better antidepressant effect than MEPS2. C-MEPS2 could exert antidepressant effects related to catecholamine synthesis with specific sites of TH, D2DR, and P-CAMKII. In addition, C-MEPS2 could repair the Res-induced damage in PC12-H cell, stabilize the mitochondrial membrane potential and regulate intracellular Ca2+ concentration, thus reducing cell apoptosis caused by RES. Antagonists common dosing experiments on animals further proved that CMEPS2 could significantly improve the antidepressant effect of derivatives without affecting the antidepressant mechanism of MEPS2. It is speculated that it may be related to carboxymethylated modification.http://www.sciencedirect.com/science/article/pii/S2213453023000617Carboxymethyl polysaccharideAnti-depressantCatecholamine |
| spellingShingle | Yu Qiu Yan Su Jia Song Fangming Mou Jia Gou Xiaoqi Geng Xinyue Li Zhiqiang Nie Jianxin Wang Yu Zheng Min Wang Carboxymethylation of the polysaccharide from the fermentation broth of Marasmius androsaceus and its antidepressant mechanisms Food Science and Human Wellness Carboxymethyl polysaccharide Anti-depressant Catecholamine |
| title | Carboxymethylation of the polysaccharide from the fermentation broth of Marasmius androsaceus and its antidepressant mechanisms |
| title_full | Carboxymethylation of the polysaccharide from the fermentation broth of Marasmius androsaceus and its antidepressant mechanisms |
| title_fullStr | Carboxymethylation of the polysaccharide from the fermentation broth of Marasmius androsaceus and its antidepressant mechanisms |
| title_full_unstemmed | Carboxymethylation of the polysaccharide from the fermentation broth of Marasmius androsaceus and its antidepressant mechanisms |
| title_short | Carboxymethylation of the polysaccharide from the fermentation broth of Marasmius androsaceus and its antidepressant mechanisms |
| title_sort | carboxymethylation of the polysaccharide from the fermentation broth of marasmius androsaceus and its antidepressant mechanisms |
| topic | Carboxymethyl polysaccharide Anti-depressant Catecholamine |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000617 |
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