Tick-borne encephalitis: immunological indicators of possible transformation of acute process into chronic disease
Several autoimmune diseases with chronic clinical course are characterized by detection of DNA autoantibodies in patients’ serum, while there are no such IgGs in healthy donors’ blood or in patients with acute clinical course with no evidence of chronization. Tick-borne encephalitis has not been con...
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Siberian State Medical University (Tomsk)
2010-08-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/1617 |
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| author | Ye. I. Gusev T. A. Parkhomenko V. N. Buneva O. B. Doronina V. B. Doronin I. I. Generalov B. M. Doronin G. A. Nevinsky |
| author_facet | Ye. I. Gusev T. A. Parkhomenko V. N. Buneva O. B. Doronina V. B. Doronin I. I. Generalov B. M. Doronin G. A. Nevinsky |
| author_sort | Ye. I. Gusev |
| collection | DOAJ |
| description | Several autoimmune diseases with chronic clinical course are characterized by detection of DNA autoantibodies in patients’ serum, while there are no such IgGs in healthy donors’ blood or in patients with acute clinical course with no evidence of chronization. Tick-borne encephalitis has not been considered this way. Several strict criteria have been applied to show that the DNase activity is an intrinsic property of IgGs from the sera of TBE patients but not from healthy donors. The relative activity of IgGs has been shown to vary extensively from patient to patient, but most of the preparations (91%) had detectable levels of the DNase activity. Polyclonal DNase IgGs were not active in the presence of EDTA or after a dialysis against EDTA, but could be activated by several externally added metal ions, with the level of activity decreasing in the order Mn2+ + Ca2+ ≥ Mn2+ + Mg2+ ≥ Mn2+ ≥ ≥ Mg2+ + Ca2+ ≥ Ca2+ ≥ Mg2+ > Ca2+, while K+ , Na+ , Ni2+, Zn2+, and Cu2+ did not stimulate DNA hydrolysis. Affinity chromatography on DNA-cellulose separated the DNase IgGs into many subfractions with various affinities for DNA and very different levels of the relative activity. Possible reasons for catalytic diversity of polyclonal human Abzs are discussed. |
| format | Article |
| id | doaj-art-c3dd67f0ea2f46e39bd3cb388c987ad4 |
| institution | Kabale University |
| issn | 1682-0363 1819-3684 |
| language | English |
| publishDate | 2010-08-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj-art-c3dd67f0ea2f46e39bd3cb388c987ad42025-08-20T03:38:09ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842010-08-019451810.20538/1682-0363-2010-4-5-181120Tick-borne encephalitis: immunological indicators of possible transformation of acute process into chronic diseaseYe. I. Gusev0T. A. Parkhomenko1V. N. Buneva2O. B. Doronina3V. B. Doronin4I. I. Generalov5B. M. Doronin6G. A. Nevinsky7Российский государственный медицинский университетИнститут химической биологии и фундаментальной медицины СО РАН; Новосибирский государственный университетИнститут химической биологии и фундаментальной медицины СО РАН; Новосибирский государственный университетНовосибирский государственный медицинский университетИнститут терапии СО РАМНВитебский государственный медицинский университетНовосибирский государственный медицинский университетИнститут химической биологии и фундаментальной медицины СО РАН; Новосибирский государственный университетSeveral autoimmune diseases with chronic clinical course are characterized by detection of DNA autoantibodies in patients’ serum, while there are no such IgGs in healthy donors’ blood or in patients with acute clinical course with no evidence of chronization. Tick-borne encephalitis has not been considered this way. Several strict criteria have been applied to show that the DNase activity is an intrinsic property of IgGs from the sera of TBE patients but not from healthy donors. The relative activity of IgGs has been shown to vary extensively from patient to patient, but most of the preparations (91%) had detectable levels of the DNase activity. Polyclonal DNase IgGs were not active in the presence of EDTA or after a dialysis against EDTA, but could be activated by several externally added metal ions, with the level of activity decreasing in the order Mn2+ + Ca2+ ≥ Mn2+ + Mg2+ ≥ Mn2+ ≥ ≥ Mg2+ + Ca2+ ≥ Ca2+ ≥ Mg2+ > Ca2+, while K+ , Na+ , Ni2+, Zn2+, and Cu2+ did not stimulate DNA hydrolysis. Affinity chromatography on DNA-cellulose separated the DNase IgGs into many subfractions with various affinities for DNA and very different levels of the relative activity. Possible reasons for catalytic diversity of polyclonal human Abzs are discussed.https://bulletin.ssmu.ru/jour/article/view/1617viral infectionstick-borne encephalitisabzymesdna hydrolysis |
| spellingShingle | Ye. I. Gusev T. A. Parkhomenko V. N. Buneva O. B. Doronina V. B. Doronin I. I. Generalov B. M. Doronin G. A. Nevinsky Tick-borne encephalitis: immunological indicators of possible transformation of acute process into chronic disease Бюллетень сибирской медицины viral infections tick-borne encephalitis abzymes dna hydrolysis |
| title | Tick-borne encephalitis: immunological indicators of possible transformation of acute process into chronic disease |
| title_full | Tick-borne encephalitis: immunological indicators of possible transformation of acute process into chronic disease |
| title_fullStr | Tick-borne encephalitis: immunological indicators of possible transformation of acute process into chronic disease |
| title_full_unstemmed | Tick-borne encephalitis: immunological indicators of possible transformation of acute process into chronic disease |
| title_short | Tick-borne encephalitis: immunological indicators of possible transformation of acute process into chronic disease |
| title_sort | tick borne encephalitis immunological indicators of possible transformation of acute process into chronic disease |
| topic | viral infections tick-borne encephalitis abzymes dna hydrolysis |
| url | https://bulletin.ssmu.ru/jour/article/view/1617 |
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