Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis
Summary: Here, we present a protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis. We describe steps for Leishmania infection, harvesting infected tissues, and performing liquid chromatography-mass spectrometry (LC-MS) to identify enriched metabolic...
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| Main Authors: | Thalia Pacheco-Fernandez, Laura Klenow, Nazli Azodi, Hannah Markle, Matthew Bernier, Greta Volpedo, Timur Oljuskin, Parna Bhattacharya, Shinjiro Hamano, Greg Matlashewski, Hira L. Nakhasi, Abhay R. Satoskar, Sreenivas Gannavaram |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002230 |
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