Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis

Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis

Summary: Here, we present a protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis. We describe steps for Leishmania infection, harvesting infected tissues, and performing liquid chromatography-mass spectrometry (LC-MS) to identify enriched metabolic...

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Bibliographic Details
Main Authors: Thalia Pacheco-Fernandez, Laura Klenow, Nazli Azodi, Hannah Markle, Matthew Bernier, Greta Volpedo, Timur Oljuskin, Parna Bhattacharya, Shinjiro Hamano, Greg Matlashewski, Hira L. Nakhasi, Abhay R. Satoskar, Sreenivas Gannavaram
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
metabolomics
microbiology
model organisms
molecular biology
mass spectrometry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725002230
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Summary:Summary: Here, we present a protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis. We describe steps for Leishmania infection, harvesting infected tissues, and performing liquid chromatography-mass spectrometry (LC-MS) to identify enriched metabolic pathways and altered metabolites. We then detail procedures for verifying the metabolite role in immune response via PCR and ELISA. This protocol focuses on cutaneous leishmaniasis models, but the analyses here are applicable to other infection models.For complete details on the use and execution of this protocol, please refer to Volpedo et al.1 and Oljuskin et al.2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667

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