Swift and portable detection of soybean mosaic virus SC7 through RNA extraction and loop-mediated isothermal amplification using lateral flow device
The soybean mosaic disease—caused by the soybean mosaic virus (SMV)—significantly impacts soybean quality and yield. Among its various strains, SMV-SC7 is prevalent in China. Therefore, rapid and accurate diagnosis is deemed critical to mitigate the spread of SMV-SC7. In this study, a simple and rap...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1478218/full |
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| author | Shui-Xian Guo Qing Zhang Nan-Nan Bai Pei-Yao Yue Jing-Ping Niu Cong-Cong Yin Ai-Qin Yue Wei-Jun Du Jin-Zhong Zhao |
| author_facet | Shui-Xian Guo Qing Zhang Nan-Nan Bai Pei-Yao Yue Jing-Ping Niu Cong-Cong Yin Ai-Qin Yue Wei-Jun Du Jin-Zhong Zhao |
| author_sort | Shui-Xian Guo |
| collection | DOAJ |
| description | The soybean mosaic disease—caused by the soybean mosaic virus (SMV)—significantly impacts soybean quality and yield. Among its various strains, SMV-SC7 is prevalent in China. Therefore, rapid and accurate diagnosis is deemed critical to mitigate the spread of SMV-SC7. In this study, a simple and rapid magnetic bead-based RNA extraction method was optimized. Furthermore, a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay that requires no specialized equipment such as PCR Amplifier was proposed, employing a lateral flow device (LFD) for visual interpretation of SMV-SC7. The RT-LAMP-LFD approach facilitated specificity testing of SMV-SC7. Moreover, the limit of detection (LOD) of this method was as low as 10−5 ng (2.4 copies). The sensitivity of RT-LAMP-LFD was 10-fold higher than that of the colorimetric RT-LAMP method. In 194 field samples tested, the RT-LAMP-LFD detection of the SMV-SC7 had accuracy of 98.45% in comparison to RT-qPCR. In conclusion, the assay exhibited high specificity, sensitivity, and rapidity, enabling economical and portable detection of SMV-SC7 and providing technical support to identify SMV-SC7-infected soybeans. |
| format | Article |
| id | doaj-art-c3d97867fa5445d4b9227a880242a409 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-c3d97867fa5445d4b9227a880242a4092025-01-03T06:47:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.14782181478218Swift and portable detection of soybean mosaic virus SC7 through RNA extraction and loop-mediated isothermal amplification using lateral flow deviceShui-Xian Guo0Qing Zhang1Nan-Nan Bai2Pei-Yao Yue3Jing-Ping Niu4Cong-Cong Yin5Ai-Qin Yue6Wei-Jun Du7Jin-Zhong Zhao8Department of Basic Sciences, Shanxi Agricultural University, Taigu, Shanxi, ChinaDepartment of Basic Sciences, Shanxi Agricultural University, Taigu, Shanxi, ChinaDepartment of Basic Sciences, Shanxi Agricultural University, Taigu, Shanxi, ChinaCollege of Agronomy, Shanxi Agricultural University, Taigu, Shanxi, ChinaCollege of Life Science, Shanxi Agricultural University, Taigu, Shanxi, ChinaDepartment of Basic Sciences, Shanxi Agricultural University, Taigu, Shanxi, ChinaCollege of Agronomy, Shanxi Agricultural University, Taigu, Shanxi, ChinaCollege of Agronomy, Shanxi Agricultural University, Taigu, Shanxi, ChinaDepartment of Basic Sciences, Shanxi Agricultural University, Taigu, Shanxi, ChinaThe soybean mosaic disease—caused by the soybean mosaic virus (SMV)—significantly impacts soybean quality and yield. Among its various strains, SMV-SC7 is prevalent in China. Therefore, rapid and accurate diagnosis is deemed critical to mitigate the spread of SMV-SC7. In this study, a simple and rapid magnetic bead-based RNA extraction method was optimized. Furthermore, a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay that requires no specialized equipment such as PCR Amplifier was proposed, employing a lateral flow device (LFD) for visual interpretation of SMV-SC7. The RT-LAMP-LFD approach facilitated specificity testing of SMV-SC7. Moreover, the limit of detection (LOD) of this method was as low as 10−5 ng (2.4 copies). The sensitivity of RT-LAMP-LFD was 10-fold higher than that of the colorimetric RT-LAMP method. In 194 field samples tested, the RT-LAMP-LFD detection of the SMV-SC7 had accuracy of 98.45% in comparison to RT-qPCR. In conclusion, the assay exhibited high specificity, sensitivity, and rapidity, enabling economical and portable detection of SMV-SC7 and providing technical support to identify SMV-SC7-infected soybeans.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1478218/fullsoybeansoybean mosaic virusvirulent strain SC7magnetic beads-based RNA extraction methodLAMPlateral flow device |
| spellingShingle | Shui-Xian Guo Qing Zhang Nan-Nan Bai Pei-Yao Yue Jing-Ping Niu Cong-Cong Yin Ai-Qin Yue Wei-Jun Du Jin-Zhong Zhao Swift and portable detection of soybean mosaic virus SC7 through RNA extraction and loop-mediated isothermal amplification using lateral flow device Frontiers in Microbiology soybean soybean mosaic virus virulent strain SC7 magnetic beads-based RNA extraction method LAMP lateral flow device |
| title | Swift and portable detection of soybean mosaic virus SC7 through RNA extraction and loop-mediated isothermal amplification using lateral flow device |
| title_full | Swift and portable detection of soybean mosaic virus SC7 through RNA extraction and loop-mediated isothermal amplification using lateral flow device |
| title_fullStr | Swift and portable detection of soybean mosaic virus SC7 through RNA extraction and loop-mediated isothermal amplification using lateral flow device |
| title_full_unstemmed | Swift and portable detection of soybean mosaic virus SC7 through RNA extraction and loop-mediated isothermal amplification using lateral flow device |
| title_short | Swift and portable detection of soybean mosaic virus SC7 through RNA extraction and loop-mediated isothermal amplification using lateral flow device |
| title_sort | swift and portable detection of soybean mosaic virus sc7 through rna extraction and loop mediated isothermal amplification using lateral flow device |
| topic | soybean soybean mosaic virus virulent strain SC7 magnetic beads-based RNA extraction method LAMP lateral flow device |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1478218/full |
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