Isolation, characterization, and receptor-binding protein specificity of phages PAS7, PAS59 and PAS61 infecting Shiga toxin-producing Escherichia coli O103 and O146
Abstract Shiga toxin-producing Escherichia coli (STEC) is a foodborne pathogen with 6,534 annual reported cases in the EU in 2021. This pathotype generally contains strains with smooth LPS with O-antigen serogroup O157 being the predominant serogroup in the US. However, non-O157 STEC serogroups are...
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Nature Portfolio
2024-10-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-77463-x |
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| author | Célia Pas Lars Fieseler Joël F. Pothier Yves Briers |
| author_facet | Célia Pas Lars Fieseler Joël F. Pothier Yves Briers |
| author_sort | Célia Pas |
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| description | Abstract Shiga toxin-producing Escherichia coli (STEC) is a foodborne pathogen with 6,534 annual reported cases in the EU in 2021. This pathotype generally contains strains with smooth LPS with O-antigen serogroup O157 being the predominant serogroup in the US. However, non-O157 STEC serogroups are becoming increasingly prevalent. Here we announce the complete genomes of three newly isolated phages that infect STEC serogroups O103 and O146, namely Escherichia phages vB_EcoP_PAS7, vB_EcoP_PAS59 and vB_EcoP_PAS61. The genome sequences revealed that they belong to three distinct genera, namely the newly proposed genus Cepavirus within the Slopekvirinae subfamily, the genus Suseptimavirus and the genus Uetakevirus, respectively. We identified the tailspikes of phages PAS7 and PAS61 as a primary specificity determinant for the O-antigens O103 and O146, respectively, and predicted their active site in silico. |
| format | Article |
| id | doaj-art-c3d61e6e5c8b4b3986f6ead9b8ac9f21 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-c3d61e6e5c8b4b3986f6ead9b8ac9f212025-08-20T02:18:28ZengNature PortfolioScientific Reports2045-23222024-10-0114111210.1038/s41598-024-77463-xIsolation, characterization, and receptor-binding protein specificity of phages PAS7, PAS59 and PAS61 infecting Shiga toxin-producing Escherichia coli O103 and O146Célia Pas0Lars Fieseler1Joël F. Pothier2Yves Briers3Department of Biotechnology, Ghent UniversityFood Microbiology Research Group, Institute of Food and Beverage Innovation, Zurich University of Applied Sciences (ZHAW)Environmental Genomics and Systems Biology Research Group, Institute of Natural Resource Sciences, Zurich University of Applied Sciences (ZHAW)Department of Biotechnology, Ghent UniversityAbstract Shiga toxin-producing Escherichia coli (STEC) is a foodborne pathogen with 6,534 annual reported cases in the EU in 2021. This pathotype generally contains strains with smooth LPS with O-antigen serogroup O157 being the predominant serogroup in the US. However, non-O157 STEC serogroups are becoming increasingly prevalent. Here we announce the complete genomes of three newly isolated phages that infect STEC serogroups O103 and O146, namely Escherichia phages vB_EcoP_PAS7, vB_EcoP_PAS59 and vB_EcoP_PAS61. The genome sequences revealed that they belong to three distinct genera, namely the newly proposed genus Cepavirus within the Slopekvirinae subfamily, the genus Suseptimavirus and the genus Uetakevirus, respectively. We identified the tailspikes of phages PAS7 and PAS61 as a primary specificity determinant for the O-antigens O103 and O146, respectively, and predicted their active site in silico.https://doi.org/10.1038/s41598-024-77463-xBacteriophageShiga toxin-producing Escherichia coli (STEC)Enterohemorrhagic Escherichia coli (EHEC)Phage isolationO103O146 |
| spellingShingle | Célia Pas Lars Fieseler Joël F. Pothier Yves Briers Isolation, characterization, and receptor-binding protein specificity of phages PAS7, PAS59 and PAS61 infecting Shiga toxin-producing Escherichia coli O103 and O146 Scientific Reports Bacteriophage Shiga toxin-producing Escherichia coli (STEC) Enterohemorrhagic Escherichia coli (EHEC) Phage isolation O103 O146 |
| title | Isolation, characterization, and receptor-binding protein specificity of phages PAS7, PAS59 and PAS61 infecting Shiga toxin-producing Escherichia coli O103 and O146 |
| title_full | Isolation, characterization, and receptor-binding protein specificity of phages PAS7, PAS59 and PAS61 infecting Shiga toxin-producing Escherichia coli O103 and O146 |
| title_fullStr | Isolation, characterization, and receptor-binding protein specificity of phages PAS7, PAS59 and PAS61 infecting Shiga toxin-producing Escherichia coli O103 and O146 |
| title_full_unstemmed | Isolation, characterization, and receptor-binding protein specificity of phages PAS7, PAS59 and PAS61 infecting Shiga toxin-producing Escherichia coli O103 and O146 |
| title_short | Isolation, characterization, and receptor-binding protein specificity of phages PAS7, PAS59 and PAS61 infecting Shiga toxin-producing Escherichia coli O103 and O146 |
| title_sort | isolation characterization and receptor binding protein specificity of phages pas7 pas59 and pas61 infecting shiga toxin producing escherichia coli o103 and o146 |
| topic | Bacteriophage Shiga toxin-producing Escherichia coli (STEC) Enterohemorrhagic Escherichia coli (EHEC) Phage isolation O103 O146 |
| url | https://doi.org/10.1038/s41598-024-77463-x |
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