Protocol for spatial metabolomics and isotope tracing in the mouse brain
Summary: Mass spectrometry imaging enables high-resolution spatial chemical mapping, yet its application for dynamic analysis with tracers poses challenges. Here, we present a protocol for spatial metabolomics and isotope tracing in the mouse brain. We describe steps for tracer administration, tissu...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725001224 |
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| author | Watit Sontising Francine Yanchik-Slade Carlos Rodriguez-Navas Md Amir Hossen Manuel Gonzalez-Rodriguez Jake Vaynshteyn Shinichi Yamaguchi Mohamed Nazim Boutaghou Isaac Marin-Valencia |
| author_facet | Watit Sontising Francine Yanchik-Slade Carlos Rodriguez-Navas Md Amir Hossen Manuel Gonzalez-Rodriguez Jake Vaynshteyn Shinichi Yamaguchi Mohamed Nazim Boutaghou Isaac Marin-Valencia |
| author_sort | Watit Sontising |
| collection | DOAJ |
| description | Summary: Mass spectrometry imaging enables high-resolution spatial chemical mapping, yet its application for dynamic analysis with tracers poses challenges. Here, we present a protocol for spatial metabolomics and isotope tracing in the mouse brain. We describe steps for tracer administration, tissue collection, and cryosectioning. We then detail procedures for matrix application, ion identification, and data analysis. This protocol delivers high-quality spatial metabolomics data and is well suited for region-specific tracing analysis in the brain. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-c3c2bd1650d9458ca3c8c582ea82f47d |
| institution | OA Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-c3c2bd1650d9458ca3c8c582ea82f47d2025-08-20T02:09:55ZengElsevierSTAR Protocols2666-16672025-06-016210371610.1016/j.xpro.2025.103716Protocol for spatial metabolomics and isotope tracing in the mouse brainWatit Sontising0Francine Yanchik-Slade1Carlos Rodriguez-Navas2Md Amir Hossen3Manuel Gonzalez-Rodriguez4Jake Vaynshteyn5Shinichi Yamaguchi6Mohamed Nazim Boutaghou7Isaac Marin-Valencia8The Abimael Laboratory of Neurometabolism, Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, NY, USA; Corresponding authorShimadzu Scientific Instruments, Columbia, MD, USA; Corresponding authorThe Abimael Laboratory of Neurometabolism, Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, NY, USAShimadzu Scientific Instruments, Columbia, MD, USAThe Abimael Laboratory of Neurometabolism, Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, NY, USAThe Abimael Laboratory of Neurometabolism, Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, NY, USAShimadzu Scientific Instruments, Columbia, MD, USAShimadzu Scientific Instruments, Columbia, MD, USAThe Abimael Laboratory of Neurometabolism, Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, NY, USA; Departments of Neuroscience, Genetics and Genomics Medicine, and Pediatrics Icahn School of Medicine at Mount Sinai, New York, NY, USA; Corresponding authorSummary: Mass spectrometry imaging enables high-resolution spatial chemical mapping, yet its application for dynamic analysis with tracers poses challenges. Here, we present a protocol for spatial metabolomics and isotope tracing in the mouse brain. We describe steps for tracer administration, tissue collection, and cryosectioning. We then detail procedures for matrix application, ion identification, and data analysis. This protocol delivers high-quality spatial metabolomics data and is well suited for region-specific tracing analysis in the brain. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725001224metabolismneurosciencemass spectrometry |
| spellingShingle | Watit Sontising Francine Yanchik-Slade Carlos Rodriguez-Navas Md Amir Hossen Manuel Gonzalez-Rodriguez Jake Vaynshteyn Shinichi Yamaguchi Mohamed Nazim Boutaghou Isaac Marin-Valencia Protocol for spatial metabolomics and isotope tracing in the mouse brain STAR Protocols metabolism neuroscience mass spectrometry |
| title | Protocol for spatial metabolomics and isotope tracing in the mouse brain |
| title_full | Protocol for spatial metabolomics and isotope tracing in the mouse brain |
| title_fullStr | Protocol for spatial metabolomics and isotope tracing in the mouse brain |
| title_full_unstemmed | Protocol for spatial metabolomics and isotope tracing in the mouse brain |
| title_short | Protocol for spatial metabolomics and isotope tracing in the mouse brain |
| title_sort | protocol for spatial metabolomics and isotope tracing in the mouse brain |
| topic | metabolism neuroscience mass spectrometry |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725001224 |
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