Development of a colloidal gold immunoassay based on monoclonal antibody for tomato spotted wilt disease
Tomato spotted wilt virus (TSWV) is a bacterial virus that seriously harms the growth of tobacco. In order to realize the rapid detection of TSWV, monoclonal antibody to TSWV-NP protein was prepared and characterized, and colloidal gold immunochromatographic test strips were established that can be...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2024-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2024/61/bioconf_isaeb2024_03010.pdf |
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| author | Wang Chunqiong Zhu Dan Sun Haowei Meng Hongming Cai Jieyun Zhang Ke Zhang Xiaowei Long Jie Meng Hongming Liu Kai Chen Dan |
| author_facet | Wang Chunqiong Zhu Dan Sun Haowei Meng Hongming Cai Jieyun Zhang Ke Zhang Xiaowei Long Jie Meng Hongming Liu Kai Chen Dan |
| author_sort | Wang Chunqiong |
| collection | DOAJ |
| description | Tomato spotted wilt virus (TSWV) is a bacterial virus that seriously harms the growth of tobacco. In order to realize the rapid detection of TSWV, monoclonal antibody to TSWV-NP protein was prepared and characterized, and colloidal gold immunochromatographic test strips were established that can be used to detect TSWV. In this study, the monoclonal antibody to TSWV-NP protein was labeled with colloidal gold, and the reaction conditions were optimized to establish a colloidal gold immunochromatographic test strip assay, and the detectability, accuracy, and specificity of the test strip were evaluated. The detection limit of the test strip for TSWV-NP protein was 0.1 μg/mL, and there was no cross-reactivity with tobacco bunchy top virus, potato Y virus, and tobacco mosaic virus, which had excellent specificity. The results of the colloidal gold test strip and the RT-PCR result method were compared with the actual samples, and the total conformity rate of the two was 95%, and the positive conformity rate was 93.3%. |
| format | Article |
| id | doaj-art-c3bb00c1adbb49c2894ff31350b9fb9d |
| institution | OA Journals |
| issn | 2117-4458 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj-art-c3bb00c1adbb49c2894ff31350b9fb9d2025-08-20T02:36:09ZengEDP SciencesBIO Web of Conferences2117-44582024-01-011420301010.1051/bioconf/202414203010bioconf_isaeb2024_03010Development of a colloidal gold immunoassay based on monoclonal antibody for tomato spotted wilt diseaseWang Chunqiong0Zhu Dan1Sun Haowei2Meng Hongming3Cai Jieyun4Zhang Ke5Zhang Xiaowei6Long Jie7Meng Hongming8Liu Kai9Chen Dan10Yunnan Tobacco Quality Supervision and Inspection StationKunming Cigarette Factory of Hongyun Honghe GroupYunnan Tobacco Quality Supervision and Inspection StationYunnan Tobacco Quality Supervision and Inspection StationYunnan Tobacco Quality Supervision and Inspection StationYunnan Tobacco Quality Supervision and Inspection StationYunnan Tobacco Quality Supervision and Inspection StationYunnan Tobacco Quality Supervision and Inspection StationYunnan Tobacco Quality Supervision and Inspection StationYunnan Tobacco Quality Supervision and Inspection StationYunnan Tobacco Quality Supervision and Inspection StationTomato spotted wilt virus (TSWV) is a bacterial virus that seriously harms the growth of tobacco. In order to realize the rapid detection of TSWV, monoclonal antibody to TSWV-NP protein was prepared and characterized, and colloidal gold immunochromatographic test strips were established that can be used to detect TSWV. In this study, the monoclonal antibody to TSWV-NP protein was labeled with colloidal gold, and the reaction conditions were optimized to establish a colloidal gold immunochromatographic test strip assay, and the detectability, accuracy, and specificity of the test strip were evaluated. The detection limit of the test strip for TSWV-NP protein was 0.1 μg/mL, and there was no cross-reactivity with tobacco bunchy top virus, potato Y virus, and tobacco mosaic virus, which had excellent specificity. The results of the colloidal gold test strip and the RT-PCR result method were compared with the actual samples, and the total conformity rate of the two was 95%, and the positive conformity rate was 93.3%.https://www.bio-conferences.org/articles/bioconf/pdf/2024/61/bioconf_isaeb2024_03010.pdf |
| spellingShingle | Wang Chunqiong Zhu Dan Sun Haowei Meng Hongming Cai Jieyun Zhang Ke Zhang Xiaowei Long Jie Meng Hongming Liu Kai Chen Dan Development of a colloidal gold immunoassay based on monoclonal antibody for tomato spotted wilt disease BIO Web of Conferences |
| title | Development of a colloidal gold immunoassay based on monoclonal antibody for tomato spotted wilt disease |
| title_full | Development of a colloidal gold immunoassay based on monoclonal antibody for tomato spotted wilt disease |
| title_fullStr | Development of a colloidal gold immunoassay based on monoclonal antibody for tomato spotted wilt disease |
| title_full_unstemmed | Development of a colloidal gold immunoassay based on monoclonal antibody for tomato spotted wilt disease |
| title_short | Development of a colloidal gold immunoassay based on monoclonal antibody for tomato spotted wilt disease |
| title_sort | development of a colloidal gold immunoassay based on monoclonal antibody for tomato spotted wilt disease |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2024/61/bioconf_isaeb2024_03010.pdf |
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