Biological Characteristics, Domestication and Cultivation of Wild <i>Tyromyces kmetii</i>

To develop and utilize the wild fungi resources in Tibet, a wild fungal specimen collected from Qiangna Town, Milin County, Tibet was isolated, purified, and characterized. Through morphological examination and ITS sequence analysis, the strain—designated as X21266—was identified as <i>Tyromyc...

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Main Authors: Haichen Huang, Xiaomin Li, Xin Hu, Huijuan Sun, Junli Zhang, Xiaoping Wu, Junsheng Fu
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Horticulturae
Subjects:
Online Access:https://www.mdpi.com/2311-7524/10/11/1177
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author Haichen Huang
Xiaomin Li
Xin Hu
Huijuan Sun
Junli Zhang
Xiaoping Wu
Junsheng Fu
author_facet Haichen Huang
Xiaomin Li
Xin Hu
Huijuan Sun
Junli Zhang
Xiaoping Wu
Junsheng Fu
author_sort Haichen Huang
collection DOAJ
description To develop and utilize the wild fungi resources in Tibet, a wild fungal specimen collected from Qiangna Town, Milin County, Tibet was isolated, purified, and characterized. Through morphological examination and ITS sequence analysis, the strain—designated as X21266—was identified as <i>Tyromyces kmetii</i>. The effects of different carbon sources, nitrogen sources, pH, and temperature on the mycelial growth of <i>T. kmetii</i> under solid-state culture conditions were investigated. In addition, research on domestication cultivation was conducted. The results showed that the optimal carbon source for <i>T. kmetii</i> was fructose, the preferred nitrogen source was beef powder, and the optimal pH and temperature were pH 5.0 and 25 °C, respectively. Domestication cultivation results showed a mycelial initiation time of 34 days; primordia formation occurred after 17 days in an environment with appropriate light and low-temperature stimulation; and during the fruiting stage, a temperature of 25–28 °C and humidity above 90% were maintained, with mature fructification forming after 16 days. This study provides fundamental experimental data for the subsequent development and utilization of <i>T. kmetii</i>.
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publishDate 2024-11-01
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spelling doaj-art-c3b88583b47b44f6b9ebca1236787eec2025-08-20T02:28:14ZengMDPI AGHorticulturae2311-75242024-11-011011117710.3390/horticulturae10111177Biological Characteristics, Domestication and Cultivation of Wild <i>Tyromyces kmetii</i>Haichen Huang0Xiaomin Li1Xin Hu2Huijuan Sun3Junli Zhang4Xiaoping Wu5Junsheng Fu6College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaTibet Academy of Agricultural and Animal Husbandry Sciences, Lasa 850030, ChinaTibet Academy of Agricultural and Animal Husbandry Sciences, Lasa 850030, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaTo develop and utilize the wild fungi resources in Tibet, a wild fungal specimen collected from Qiangna Town, Milin County, Tibet was isolated, purified, and characterized. Through morphological examination and ITS sequence analysis, the strain—designated as X21266—was identified as <i>Tyromyces kmetii</i>. The effects of different carbon sources, nitrogen sources, pH, and temperature on the mycelial growth of <i>T. kmetii</i> under solid-state culture conditions were investigated. In addition, research on domestication cultivation was conducted. The results showed that the optimal carbon source for <i>T. kmetii</i> was fructose, the preferred nitrogen source was beef powder, and the optimal pH and temperature were pH 5.0 and 25 °C, respectively. Domestication cultivation results showed a mycelial initiation time of 34 days; primordia formation occurred after 17 days in an environment with appropriate light and low-temperature stimulation; and during the fruiting stage, a temperature of 25–28 °C and humidity above 90% were maintained, with mature fructification forming after 16 days. This study provides fundamental experimental data for the subsequent development and utilization of <i>T. kmetii</i>.https://www.mdpi.com/2311-7524/10/11/1177<i>Tyromyces kmetii</i>identificationbiological characteristicsdomestication cultivation
spellingShingle Haichen Huang
Xiaomin Li
Xin Hu
Huijuan Sun
Junli Zhang
Xiaoping Wu
Junsheng Fu
Biological Characteristics, Domestication and Cultivation of Wild <i>Tyromyces kmetii</i>
Horticulturae
<i>Tyromyces kmetii</i>
identification
biological characteristics
domestication cultivation
title Biological Characteristics, Domestication and Cultivation of Wild <i>Tyromyces kmetii</i>
title_full Biological Characteristics, Domestication and Cultivation of Wild <i>Tyromyces kmetii</i>
title_fullStr Biological Characteristics, Domestication and Cultivation of Wild <i>Tyromyces kmetii</i>
title_full_unstemmed Biological Characteristics, Domestication and Cultivation of Wild <i>Tyromyces kmetii</i>
title_short Biological Characteristics, Domestication and Cultivation of Wild <i>Tyromyces kmetii</i>
title_sort biological characteristics domestication and cultivation of wild i tyromyces kmetii i
topic <i>Tyromyces kmetii</i>
identification
biological characteristics
domestication cultivation
url https://www.mdpi.com/2311-7524/10/11/1177
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