Differentially expressed genes of SHED based on RNA-seq analysis after long-term expansion in vitro
[Objective:] To investigate the differences in gene expression of stem cells derived from human exfoliated deciduous teeth (SHED) after long-term continuous subculture to passage 20 (P20) in vitro by RNA sequencing (RNA-seq) analysis technology, and to preliminarily explore their signaling pathways...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Oral and Maxillofacial Surgery
2023-08-01
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| Series: | Kouqiang hemian waike zazhi |
| Subjects: | |
| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.04.002 |
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| Summary: | [Objective:] To investigate the differences in gene expression of stem cells derived from human exfoliated deciduous teeth (SHED) after long-term continuous subculture to passage 20 (P20) in vitro by RNA sequencing (RNA-seq) analysis technology, and to preliminarily explore their signaling pathways related to in vitro expansion and aging. [Methods:] SHED were isolated from deciduous teeth of healthy children in mixed dentition stage and subculture to P20 under the standard condition. RNA-seq was performed to identify the differentially expressed genes among different cell passages, then the relevant biological information was analyzed to explore the senescence related signal pathway of continuous amplification of SHED in vitro. [Results:] RNA-seq results showed that a total of 1 884 genes were differentially expressed in SHED at passage 4 (P4) and P20, including 575 up-regulated genes and 1 309 down-regulated genes. Gene ontology analysis indicated that the differentially expressed genes were distributed in biological processes (BP), cell composition (CC) and molecular function (MF). Moreover, the pathways most enriched for differentiallygenes and related proteins were mainly concentrated in the spliceosome, ribosome, cell cycle and p53 pathway related signaling pathways. [Conclusion:] This study revealed that the changes of gene expression profile of SHED after continuous amplification culture to the 20th generation in vitro, which indicated the direction for the further study of in vitro senescence mechanism of SHED. |
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| ISSN: | 1005-4979 |