Spleen-based proteogenomics reveals that Escherichia coli infection induces activation of phagosome maturation pathway in chicken
Avian pathogenic Escherichia coli (APEC) leads to economic losses in poultry industry and is also a threat to human health. Various strategies were used for searching virulence factors, while little is known about the mechanism by which APEC survives in host or is eliminated by host. Thus, chicken c...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Virulence |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2022.2150453 |
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| author | Jiahui Shi Songhao Jiang Qiang Wang Jilin Dong Huiming Zhu Peijia Wang Shuhong Meng Zhenpeng Zhang Lei Chang Guibin Wang Xiaoqin Xu Ping Xu Yao Zhang |
| author_facet | Jiahui Shi Songhao Jiang Qiang Wang Jilin Dong Huiming Zhu Peijia Wang Shuhong Meng Zhenpeng Zhang Lei Chang Guibin Wang Xiaoqin Xu Ping Xu Yao Zhang |
| author_sort | Jiahui Shi |
| collection | DOAJ |
| description | Avian pathogenic Escherichia coli (APEC) leads to economic losses in poultry industry and is also a threat to human health. Various strategies were used for searching virulence factors, while little is known about the mechanism by which APEC survives in host or is eliminated by host. Thus, chicken colibacillosis model was constructed by intraperitoneally injecting E. coli O78 in this study, then the protein dynamic expression of spleen was characterized at different post-infection times by quantitative proteome. Comparative analysis showed that E. coli induced significant dysregulation at 72 h post infection in spleen tissue. Transcriptomic method was further used to assess the changes of dysregulated proteins at 72 h post infection at the mRNA level. Total 278 protein groups (5.7%) and 2,443 genes (24.4%) were dysregulated, respectively. The upregulated proteins and genes were consistently enriched in phagosome and lysosome pathways, indicating E. coli infection activates phagosome maturation pathway. The matured phagolysosome might kill the invasive E. coli. This study illuminated the genetic dysregulation in chicken spleen at the protein and mRNA levels after E. coli infecting and identified candidate genes for host response to APEC infection. |
| format | Article |
| id | doaj-art-c3aa9a305a2e4efca31e1eefaccb9e4d |
| institution | OA Journals |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj-art-c3aa9a305a2e4efca31e1eefaccb9e4d2025-08-20T02:37:45ZengTaylor & Francis GroupVirulence2150-55942150-56082023-12-0114110.1080/21505594.2022.2150453Spleen-based proteogenomics reveals that Escherichia coli infection induces activation of phagosome maturation pathway in chickenJiahui Shi0Songhao Jiang1Qiang Wang2Jilin Dong3Huiming Zhu4Peijia Wang5Shuhong Meng6Zhenpeng Zhang7Lei Chang8Guibin Wang9Xiaoqin Xu10Ping Xu11Yao Zhang12State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing, ChinaState Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing, ChinaCollege of veterinary medicine, Yangzhou University, Yangzhou, ChinaState Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing, ChinaState Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing, ChinaCollege of veterinary medicine, Yangzhou University, Yangzhou, ChinaState Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing, ChinaState Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing, ChinaState Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing, ChinaState Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing, ChinaCollege of veterinary medicine, Yangzhou University, Yangzhou, ChinaState Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing, ChinaState Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences Beijing, Research Unit of Proteomics & Research and Development of New Drug of Chinese Academy of Medical Sciences, Institute of Lifeomics, Beijing, ChinaAvian pathogenic Escherichia coli (APEC) leads to economic losses in poultry industry and is also a threat to human health. Various strategies were used for searching virulence factors, while little is known about the mechanism by which APEC survives in host or is eliminated by host. Thus, chicken colibacillosis model was constructed by intraperitoneally injecting E. coli O78 in this study, then the protein dynamic expression of spleen was characterized at different post-infection times by quantitative proteome. Comparative analysis showed that E. coli induced significant dysregulation at 72 h post infection in spleen tissue. Transcriptomic method was further used to assess the changes of dysregulated proteins at 72 h post infection at the mRNA level. Total 278 protein groups (5.7%) and 2,443 genes (24.4%) were dysregulated, respectively. The upregulated proteins and genes were consistently enriched in phagosome and lysosome pathways, indicating E. coli infection activates phagosome maturation pathway. The matured phagolysosome might kill the invasive E. coli. This study illuminated the genetic dysregulation in chicken spleen at the protein and mRNA levels after E. coli infecting and identified candidate genes for host response to APEC infection.https://www.tandfonline.com/doi/10.1080/21505594.2022.2150453White Leghorn chickenEscherichia coli O78spleenquantitative proteometranscriptome |
| spellingShingle | Jiahui Shi Songhao Jiang Qiang Wang Jilin Dong Huiming Zhu Peijia Wang Shuhong Meng Zhenpeng Zhang Lei Chang Guibin Wang Xiaoqin Xu Ping Xu Yao Zhang Spleen-based proteogenomics reveals that Escherichia coli infection induces activation of phagosome maturation pathway in chicken Virulence White Leghorn chicken Escherichia coli O78 spleen quantitative proteome transcriptome |
| title | Spleen-based proteogenomics reveals that Escherichia coli infection induces activation of phagosome maturation pathway in chicken |
| title_full | Spleen-based proteogenomics reveals that Escherichia coli infection induces activation of phagosome maturation pathway in chicken |
| title_fullStr | Spleen-based proteogenomics reveals that Escherichia coli infection induces activation of phagosome maturation pathway in chicken |
| title_full_unstemmed | Spleen-based proteogenomics reveals that Escherichia coli infection induces activation of phagosome maturation pathway in chicken |
| title_short | Spleen-based proteogenomics reveals that Escherichia coli infection induces activation of phagosome maturation pathway in chicken |
| title_sort | spleen based proteogenomics reveals that escherichia coli infection induces activation of phagosome maturation pathway in chicken |
| topic | White Leghorn chicken Escherichia coli O78 spleen quantitative proteome transcriptome |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2022.2150453 |
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