RETRACTED ARTICLE: Interleukin-27 ameliorates coxsackievirus-B3-induced viral myocarditis by inhibiting Th17 cells
Abstract Background Interleukin (IL)-27, which has both pro and anti- inflammatory properties, is a new discovered heterodimeric cytokine that belongs to IL-12 family. However, the expression pattern and functional role of IL-27 in viral myocarditis (VMC) has not been investigated. Methods BALB/c mi...
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BMC
2015-11-01
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| Series: | Virology Journal |
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| Online Access: | https://doi.org/10.1186/s12985-015-0418-x |
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| author | Hengshan Zhu Chuang Lou Ping Liu |
| author_facet | Hengshan Zhu Chuang Lou Ping Liu |
| author_sort | Hengshan Zhu |
| collection | DOAJ |
| description | Abstract Background Interleukin (IL)-27, which has both pro and anti- inflammatory properties, is a new discovered heterodimeric cytokine that belongs to IL-12 family. However, the expression pattern and functional role of IL-27 in viral myocarditis (VMC) has not been investigated. Methods BALB/c mice were intraperitoneally (i.p) infected with Coxsackie virus B3 (CVB3) for establishing VMC models. Mice were then injected i.p. with Anti-Mouse IL-27 p28Ab or recombinant IL-27 for neutralization and overexpression of IL-27. The survival rates of mice were recorded and the kinetics of IL-27 expression, the frequencies of Th17 cells and the expression of inflammatory cytokine in CVB3-infected mice were determined by ELISA, real-time PCR and flow cytometry. Results The IL-27 expression in heart tissues and serum in coxsackievirus B3 (CVB3)-induced myocarditis mice peaked on day 4 but then rapidly decreased during the late infectious stage of CVB3, high IL-27 levels were negatively correlated with bodyweight loss (r = −0.71, P = 0.021) and myocardial pathological score (r = −0.85, P = 0.0018). Additionally, neutralization of IL-27 with Anti-IL-27 Ab accelerated, whereas systemic administration of recombinant mouse IL-27 ameliorated CVB3-induced myocarditis. The protective role of IL-27 in VMC was reflected by an improved survival rate, increased bodyweights, and reduced pathological scores in Anti-IL-27 group compared with IgG control group. Mechanistic investigations showed that IL-27 inhibited Th17 cells frequencies and IL-17 production, as well as the Th17-related proinflammatory cytokines in heart tissues. Conclusions Our results demonstrate that that IL-27 effectively protects the myocardium from the pathogenesis of CVB3 induced myocarditis, which may be attributable to reduced Th17 production. IL-27 might serve as a novel therapeutic treatment for VMC. |
| format | Article |
| id | doaj-art-c3a24978c0bb4b6eb814c10106afd742 |
| institution | Kabale University |
| issn | 1743-422X |
| language | English |
| publishDate | 2015-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj-art-c3a24978c0bb4b6eb814c10106afd7422025-08-20T03:45:27ZengBMCVirology Journal1743-422X2015-11-011211810.1186/s12985-015-0418-xRETRACTED ARTICLE: Interleukin-27 ameliorates coxsackievirus-B3-induced viral myocarditis by inhibiting Th17 cellsHengshan Zhu0Chuang Lou1Ping Liu2Department of Cardiovascular Surgery, Xianyang Hospital of Yan’an UniversityDepartment of Cardiology, AnKang Hospital of Traditional Chinese MedicineDepartment of Cardiology, Xianyang Hospital of Yan’an UniversityAbstract Background Interleukin (IL)-27, which has both pro and anti- inflammatory properties, is a new discovered heterodimeric cytokine that belongs to IL-12 family. However, the expression pattern and functional role of IL-27 in viral myocarditis (VMC) has not been investigated. Methods BALB/c mice were intraperitoneally (i.p) infected with Coxsackie virus B3 (CVB3) for establishing VMC models. Mice were then injected i.p. with Anti-Mouse IL-27 p28Ab or recombinant IL-27 for neutralization and overexpression of IL-27. The survival rates of mice were recorded and the kinetics of IL-27 expression, the frequencies of Th17 cells and the expression of inflammatory cytokine in CVB3-infected mice were determined by ELISA, real-time PCR and flow cytometry. Results The IL-27 expression in heart tissues and serum in coxsackievirus B3 (CVB3)-induced myocarditis mice peaked on day 4 but then rapidly decreased during the late infectious stage of CVB3, high IL-27 levels were negatively correlated with bodyweight loss (r = −0.71, P = 0.021) and myocardial pathological score (r = −0.85, P = 0.0018). Additionally, neutralization of IL-27 with Anti-IL-27 Ab accelerated, whereas systemic administration of recombinant mouse IL-27 ameliorated CVB3-induced myocarditis. The protective role of IL-27 in VMC was reflected by an improved survival rate, increased bodyweights, and reduced pathological scores in Anti-IL-27 group compared with IgG control group. Mechanistic investigations showed that IL-27 inhibited Th17 cells frequencies and IL-17 production, as well as the Th17-related proinflammatory cytokines in heart tissues. Conclusions Our results demonstrate that that IL-27 effectively protects the myocardium from the pathogenesis of CVB3 induced myocarditis, which may be attributable to reduced Th17 production. IL-27 might serve as a novel therapeutic treatment for VMC.https://doi.org/10.1186/s12985-015-0418-xInterleukin-27Viral myocarditisCVB3Th17 cells |
| spellingShingle | Hengshan Zhu Chuang Lou Ping Liu RETRACTED ARTICLE: Interleukin-27 ameliorates coxsackievirus-B3-induced viral myocarditis by inhibiting Th17 cells Virology Journal Interleukin-27 Viral myocarditis CVB3 Th17 cells |
| title | RETRACTED ARTICLE: Interleukin-27 ameliorates coxsackievirus-B3-induced viral myocarditis by inhibiting Th17 cells |
| title_full | RETRACTED ARTICLE: Interleukin-27 ameliorates coxsackievirus-B3-induced viral myocarditis by inhibiting Th17 cells |
| title_fullStr | RETRACTED ARTICLE: Interleukin-27 ameliorates coxsackievirus-B3-induced viral myocarditis by inhibiting Th17 cells |
| title_full_unstemmed | RETRACTED ARTICLE: Interleukin-27 ameliorates coxsackievirus-B3-induced viral myocarditis by inhibiting Th17 cells |
| title_short | RETRACTED ARTICLE: Interleukin-27 ameliorates coxsackievirus-B3-induced viral myocarditis by inhibiting Th17 cells |
| title_sort | retracted article interleukin 27 ameliorates coxsackievirus b3 induced viral myocarditis by inhibiting th17 cells |
| topic | Interleukin-27 Viral myocarditis CVB3 Th17 cells |
| url | https://doi.org/10.1186/s12985-015-0418-x |
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