Genomic signatures of sensory adaptation and evolution in pangolins

Abstract Background Pangolin is one of the most endangered mammals with many peculiar characteristics, yet the understanding of its sensory systems is still superficial. Studying the genomic basis of adaptation and evolution of pangolin’s sensory system is expected to provide further potential assis...

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Main Authors: Jun Li, Ce Guo, Meiling Xie, Kai Wang, Xianghe Wang, Bishan Zou, Fanghui Hou, Chongyang Ran, Shiman Bi, Yanchun Xu, Yan Hua
Format: Article
Language:English
Published: BMC 2024-12-01
Series:BMC Genomics
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Online Access:https://doi.org/10.1186/s12864-024-11063-x
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author Jun Li
Ce Guo
Meiling Xie
Kai Wang
Xianghe Wang
Bishan Zou
Fanghui Hou
Chongyang Ran
Shiman Bi
Yanchun Xu
Yan Hua
author_facet Jun Li
Ce Guo
Meiling Xie
Kai Wang
Xianghe Wang
Bishan Zou
Fanghui Hou
Chongyang Ran
Shiman Bi
Yanchun Xu
Yan Hua
author_sort Jun Li
collection DOAJ
description Abstract Background Pangolin is one of the most endangered mammals with many peculiar characteristics, yet the understanding of its sensory systems is still superficial. Studying the genomic basis of adaptation and evolution of pangolin’s sensory system is expected to provide further potential assistance for their conservation in the future. Results In this study, we performed a comprehensive comparative genomic analysis to explore the signature of sensory adaptation and evolution in pangolins. By comparing with the aardvark, Cape golden mole, and short-beaked echidna, 124 and 152 expanded gene families were detected in the genome of the Chinese and Malayan pangolins, respectively. The enrichment analyses showed olfactory-related genomic convergence among five concerned mammals. We found 769 and 733 intact OR genes, and 704 and 475 OR pseudogenes in the Chinese and Malayan pangolin species, respectively. Compared to other mammals, far more intact members of OR6 and OR14 were identified in pangolins, particularly for four genes with large copy numbers (OR6C2, OR14A2, OR14C36, and OR14L1). On the genome-wide scale, 1,523, 1,887, 1,110, and 2,732 genes were detected under positive selection (PSGs), intensified selection (ISGs), rapid evolution (REGs), and relaxed selection (RSGs) in pangolins. GO terms associated with visual perception were enriched in PSGs, ISGs, and REGs. Those related to rhythm and sound perception were enriched in both ISGs and REGs, ear development and morphogenesis were enriched in ISGs, and mechanical stimulus and temperature adaptation were enriched in RSGs. The convergence of two vision-related PSGs (OPN4 and ATXN7), with more than one parallel substituted site, was detected among five concerned mammals. Additionally, the absence of intact genes of PKD1L3, PKD2L1, and TAS1R2 and just six single-copy TAS2Rs (TAS2R1, TAS2R4, TAS2R7, TAS2R38, TAS2R40, and TAS2R46) were found in pangolins. Interestingly, we found two large insertions in TAS1R3, distributed in the N-terminal ectodomain, just in pangolins. Conclusions We found new features related to the adaptation and evolution of pangolin-specific sensory characteristics across the genome. These are expected to provide valuable and useful genome-wide genetic information for the future breeding and conservation of pangolins.
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spelling doaj-art-c39eceb66f324edf937b3102cbf8eeea2025-08-20T02:20:45ZengBMCBMC Genomics1471-21642024-12-0125111310.1186/s12864-024-11063-xGenomic signatures of sensory adaptation and evolution in pangolinsJun Li0Ce Guo1Meiling Xie2Kai Wang3Xianghe Wang4Bishan Zou5Fanghui Hou6Chongyang Ran7Shiman Bi8Yanchun Xu9Yan Hua10Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of ForestryGuangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of ForestryGuangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of ForestryGuangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of ForestryGuangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of ForestryGuangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of ForestryGuangdong Wildlife Rescue Monitoring CenterGuangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of ForestryGuangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of ForestryCollege of Wildlife and Protected Area, Northeast Forestry UniversityGuangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of ForestryAbstract Background Pangolin is one of the most endangered mammals with many peculiar characteristics, yet the understanding of its sensory systems is still superficial. Studying the genomic basis of adaptation and evolution of pangolin’s sensory system is expected to provide further potential assistance for their conservation in the future. Results In this study, we performed a comprehensive comparative genomic analysis to explore the signature of sensory adaptation and evolution in pangolins. By comparing with the aardvark, Cape golden mole, and short-beaked echidna, 124 and 152 expanded gene families were detected in the genome of the Chinese and Malayan pangolins, respectively. The enrichment analyses showed olfactory-related genomic convergence among five concerned mammals. We found 769 and 733 intact OR genes, and 704 and 475 OR pseudogenes in the Chinese and Malayan pangolin species, respectively. Compared to other mammals, far more intact members of OR6 and OR14 were identified in pangolins, particularly for four genes with large copy numbers (OR6C2, OR14A2, OR14C36, and OR14L1). On the genome-wide scale, 1,523, 1,887, 1,110, and 2,732 genes were detected under positive selection (PSGs), intensified selection (ISGs), rapid evolution (REGs), and relaxed selection (RSGs) in pangolins. GO terms associated with visual perception were enriched in PSGs, ISGs, and REGs. Those related to rhythm and sound perception were enriched in both ISGs and REGs, ear development and morphogenesis were enriched in ISGs, and mechanical stimulus and temperature adaptation were enriched in RSGs. The convergence of two vision-related PSGs (OPN4 and ATXN7), with more than one parallel substituted site, was detected among five concerned mammals. Additionally, the absence of intact genes of PKD1L3, PKD2L1, and TAS1R2 and just six single-copy TAS2Rs (TAS2R1, TAS2R4, TAS2R7, TAS2R38, TAS2R40, and TAS2R46) were found in pangolins. Interestingly, we found two large insertions in TAS1R3, distributed in the N-terminal ectodomain, just in pangolins. Conclusions We found new features related to the adaptation and evolution of pangolin-specific sensory characteristics across the genome. These are expected to provide valuable and useful genome-wide genetic information for the future breeding and conservation of pangolins.https://doi.org/10.1186/s12864-024-11063-xSensory systemPangolinAdaptionConvergent evolutionConservation
spellingShingle Jun Li
Ce Guo
Meiling Xie
Kai Wang
Xianghe Wang
Bishan Zou
Fanghui Hou
Chongyang Ran
Shiman Bi
Yanchun Xu
Yan Hua
Genomic signatures of sensory adaptation and evolution in pangolins
BMC Genomics
Sensory system
Pangolin
Adaption
Convergent evolution
Conservation
title Genomic signatures of sensory adaptation and evolution in pangolins
title_full Genomic signatures of sensory adaptation and evolution in pangolins
title_fullStr Genomic signatures of sensory adaptation and evolution in pangolins
title_full_unstemmed Genomic signatures of sensory adaptation and evolution in pangolins
title_short Genomic signatures of sensory adaptation and evolution in pangolins
title_sort genomic signatures of sensory adaptation and evolution in pangolins
topic Sensory system
Pangolin
Adaption
Convergent evolution
Conservation
url https://doi.org/10.1186/s12864-024-11063-x
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