Palm Tocotrienol Supplementation Enhanced Bone Formation in Oestrogen-Deficient Rats

Postmenopausal osteoporosis is the commonest cause of osteoporosis. It is associated with increased free radical activity induced by the oestrogen-deficient state. Therefore, supplementation with palm-oil-derived tocotrienols, a potent antioxidant, should be able to prevent this bone loss. Our earl...

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Main Authors: Ima Nirwana Soelaiman, Wang Ming, Roshayati Abu Bakar, Nursyahrina Atiqah Hashnan, Hanif Mohd Ali, Norazlina Mohamed, Norliza Muhammad, Ahmad Nazrun Shuid
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:International Journal of Endocrinology
Online Access:http://dx.doi.org/10.1155/2012/532862
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author Ima Nirwana Soelaiman
Wang Ming
Roshayati Abu Bakar
Nursyahrina Atiqah Hashnan
Hanif Mohd Ali
Norazlina Mohamed
Norliza Muhammad
Ahmad Nazrun Shuid
author_facet Ima Nirwana Soelaiman
Wang Ming
Roshayati Abu Bakar
Nursyahrina Atiqah Hashnan
Hanif Mohd Ali
Norazlina Mohamed
Norliza Muhammad
Ahmad Nazrun Shuid
author_sort Ima Nirwana Soelaiman
collection DOAJ
description Postmenopausal osteoporosis is the commonest cause of osteoporosis. It is associated with increased free radical activity induced by the oestrogen-deficient state. Therefore, supplementation with palm-oil-derived tocotrienols, a potent antioxidant, should be able to prevent this bone loss. Our earlier studies have shown that tocotrienol was able to prevent and even reverse osteoporosis due to various factors, including oestrogen deficiency. In this study we compared the effects of supplementation with palm tocotrienol mixture or calcium on bone biomarkers and bone formation rate in ovariectomised (oestrogen-deficient) female rats. Our results showed that palm tocotrienols significantly increased bone formation in oestrogen-deficient rats, seen by increased double-labeled surface (dLS/Bs), reduced single-labeled surface (sLS/BS), increased mineralizing surface (MS/BS), increased mineral apposition rate (MAR), and an overall increase in bone formation rate (BFR/BS). These effects were not seen in the group supplemented with calcium. However, no significant changes were seen in the serum levels of the bone biomarkers, osteocalcin, and cross-linked C-telopeptide of type I collagen, CTX. In conclusion, palm tocotrienol is more effective than calcium in preventing oestrogen-deficient bone loss. Further studies are needed to determine the potential of tocotrienol as an antiosteoporotic agent.
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spelling doaj-art-c39ccc83e1bc45169f2df339a41b3e852025-08-20T03:23:12ZengWileyInternational Journal of Endocrinology1687-83371687-83452012-01-01201210.1155/2012/532862532862Palm Tocotrienol Supplementation Enhanced Bone Formation in Oestrogen-Deficient RatsIma Nirwana Soelaiman0Wang Ming1Roshayati Abu Bakar2Nursyahrina Atiqah Hashnan3Hanif Mohd Ali4Norazlina Mohamed5Norliza Muhammad6Ahmad Nazrun Shuid7Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, MalaysiaDepartment of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, MalaysiaPostmenopausal osteoporosis is the commonest cause of osteoporosis. It is associated with increased free radical activity induced by the oestrogen-deficient state. Therefore, supplementation with palm-oil-derived tocotrienols, a potent antioxidant, should be able to prevent this bone loss. Our earlier studies have shown that tocotrienol was able to prevent and even reverse osteoporosis due to various factors, including oestrogen deficiency. In this study we compared the effects of supplementation with palm tocotrienol mixture or calcium on bone biomarkers and bone formation rate in ovariectomised (oestrogen-deficient) female rats. Our results showed that palm tocotrienols significantly increased bone formation in oestrogen-deficient rats, seen by increased double-labeled surface (dLS/Bs), reduced single-labeled surface (sLS/BS), increased mineralizing surface (MS/BS), increased mineral apposition rate (MAR), and an overall increase in bone formation rate (BFR/BS). These effects were not seen in the group supplemented with calcium. However, no significant changes were seen in the serum levels of the bone biomarkers, osteocalcin, and cross-linked C-telopeptide of type I collagen, CTX. In conclusion, palm tocotrienol is more effective than calcium in preventing oestrogen-deficient bone loss. Further studies are needed to determine the potential of tocotrienol as an antiosteoporotic agent.http://dx.doi.org/10.1155/2012/532862
spellingShingle Ima Nirwana Soelaiman
Wang Ming
Roshayati Abu Bakar
Nursyahrina Atiqah Hashnan
Hanif Mohd Ali
Norazlina Mohamed
Norliza Muhammad
Ahmad Nazrun Shuid
Palm Tocotrienol Supplementation Enhanced Bone Formation in Oestrogen-Deficient Rats
International Journal of Endocrinology
title Palm Tocotrienol Supplementation Enhanced Bone Formation in Oestrogen-Deficient Rats
title_full Palm Tocotrienol Supplementation Enhanced Bone Formation in Oestrogen-Deficient Rats
title_fullStr Palm Tocotrienol Supplementation Enhanced Bone Formation in Oestrogen-Deficient Rats
title_full_unstemmed Palm Tocotrienol Supplementation Enhanced Bone Formation in Oestrogen-Deficient Rats
title_short Palm Tocotrienol Supplementation Enhanced Bone Formation in Oestrogen-Deficient Rats
title_sort palm tocotrienol supplementation enhanced bone formation in oestrogen deficient rats
url http://dx.doi.org/10.1155/2012/532862
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