Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters
Introduction: Uropathogenic Escherichia coli (UPEC), an important causative agent of urinary tract infections (UTIs), carries virulence factors which are clustered on pathogenicity islands (PAIs). The goal of this study was to characterize the PAIs among the UPEC isolated from patients with urinary...
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The Journal of Infection in Developing Countries
2017-07-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/8660 |
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| author | Farzaneh Firoozeh Leila Soleimani-Moorchekhorti Mohammad Zibaei |
| author_facet | Farzaneh Firoozeh Leila Soleimani-Moorchekhorti Mohammad Zibaei |
| author_sort | Farzaneh Firoozeh |
| collection | DOAJ |
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Introduction: Uropathogenic Escherichia coli (UPEC), an important causative agent of urinary tract infections (UTIs), carries virulence factors which are clustered on pathogenicity islands (PAIs). The goal of this study was to characterize the PAIs among the UPEC isolated from patients with urinary catheters.
Methodology: A descriptive cross-sectional study was designed and from December 2014 to April 2015, 78 non-duplicate E. coli were collected from hospitalized patients with UTIs, including patients with and without indwelling urinary catheters. Two multiplex polymerase chain reaction (PCR) assays were performed to evaluate the presence of the eight most studied PAIs (I 536, II 536, III 536, IV536, I CFT073, II CFT073, PAI IJ96, and PAI IIJ96).
Results: Of 78 patients with a UTI 31 (39.7%) used indwelling catheters. Of these 31 patients, 27 (87.1%) carried PAIs markers, including 25 (80.6%) PAI IV536, 12 (38.7%) PAI ICFT073, 6 (19.4%) PAI IICFT073, 6 (19.4%) PAI III536 and 3 (9.7%) PAI II536. PAI I536, PAI IJ96, and PAI IIJ96 were not detected in the UPEC strains.
Conclusions: The findings of this study revealed that the frequency of PAI markers in UPEC isolates from patients with indwelling urinary catheters was high. The rate of multiple PAIs carriage was notable among those patients, suggesting that UPEC strains that colonize the indwelling urinary catheters have the potential to cause complicated urinary infections. PAI ICFT073, which was found in association with pyelonephritis, prostatitis, and sepsis, could be considered as a target for medical interventions.
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| format | Article |
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| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2017-07-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-c399d4a267774bdb81b334bb6f814ebb2025-08-20T02:57:01ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802017-07-01110710.3855/jidc.8660Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary cathetersFarzaneh Firoozeh0Leila Soleimani-Moorchekhorti1Mohammad Zibaei2School of Medicine, Kashan University of Medical Sciences, Kashan, I.R. IranSchool of Medicine, Kashan University of Medical Sciences, Kashan, I.R. IranSchool of Medicine, Alborz University of Medical Sciences, Karaj, I.R. Iran Introduction: Uropathogenic Escherichia coli (UPEC), an important causative agent of urinary tract infections (UTIs), carries virulence factors which are clustered on pathogenicity islands (PAIs). The goal of this study was to characterize the PAIs among the UPEC isolated from patients with urinary catheters. Methodology: A descriptive cross-sectional study was designed and from December 2014 to April 2015, 78 non-duplicate E. coli were collected from hospitalized patients with UTIs, including patients with and without indwelling urinary catheters. Two multiplex polymerase chain reaction (PCR) assays were performed to evaluate the presence of the eight most studied PAIs (I 536, II 536, III 536, IV536, I CFT073, II CFT073, PAI IJ96, and PAI IIJ96). Results: Of 78 patients with a UTI 31 (39.7%) used indwelling catheters. Of these 31 patients, 27 (87.1%) carried PAIs markers, including 25 (80.6%) PAI IV536, 12 (38.7%) PAI ICFT073, 6 (19.4%) PAI IICFT073, 6 (19.4%) PAI III536 and 3 (9.7%) PAI II536. PAI I536, PAI IJ96, and PAI IIJ96 were not detected in the UPEC strains. Conclusions: The findings of this study revealed that the frequency of PAI markers in UPEC isolates from patients with indwelling urinary catheters was high. The rate of multiple PAIs carriage was notable among those patients, suggesting that UPEC strains that colonize the indwelling urinary catheters have the potential to cause complicated urinary infections. PAI ICFT073, which was found in association with pyelonephritis, prostatitis, and sepsis, could be considered as a target for medical interventions. https://jidc.org/index.php/journal/article/view/8660uropathogenic Escherichia coliUropathogenicpathogenicity islandsEscherichia coliurinary catheter |
| spellingShingle | Farzaneh Firoozeh Leila Soleimani-Moorchekhorti Mohammad Zibaei Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters Journal of Infection in Developing Countries uropathogenic Escherichia coli Uropathogenic pathogenicity islands Escherichia coli urinary catheter |
| title | Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters |
| title_full | Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters |
| title_fullStr | Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters |
| title_full_unstemmed | Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters |
| title_short | Evaluation of pathogenicity islands in uropathogenic Escherichia coli isolated from patients with urinary catheters |
| title_sort | evaluation of pathogenicity islands in uropathogenic escherichia coli isolated from patients with urinary catheters |
| topic | uropathogenic Escherichia coli Uropathogenic pathogenicity islands Escherichia coli urinary catheter |
| url | https://jidc.org/index.php/journal/article/view/8660 |
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