Immune Response following Liver Transplantation Compared to Kidney Transplantation: Usefulness of Monitoring Peripheral Blood CD4+ Adenosine Triphosphate Activity and Cytochrome P450 3A5 Genotype Assay
Seventy living donor liver transplantation (LDLT) and 39 kidney transplantation (KT) patients were randomly screened by using the peripheral blood CD4+ adenosine triphosphate activity (ATP) assay (IMK assay). The patients were divided into 2 groups in each organ transplantation with low IMK ATP leve...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/936063 |
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| author | Yu Nobuoka Shugo Mizuno Kouhei Nishikawa Kaname Nakatani Yuichi Muraki Tomomi Yamada Masahiro Okuda Tsutomu Nobori Yoshiki Sugimura Shuji Isaji |
| author_facet | Yu Nobuoka Shugo Mizuno Kouhei Nishikawa Kaname Nakatani Yuichi Muraki Tomomi Yamada Masahiro Okuda Tsutomu Nobori Yoshiki Sugimura Shuji Isaji |
| author_sort | Yu Nobuoka |
| collection | DOAJ |
| description | Seventy living donor liver transplantation (LDLT) and 39 kidney transplantation (KT) patients were randomly screened by using the peripheral blood CD4+ adenosine triphosphate activity (ATP) assay (IMK assay). The patients were divided into 2 groups in each organ transplantation with low IMK ATP level (<225 ng/mL) or high (>225) (LT-L: n=23, KT-L: n=19, LT-H: n=47, and KT-H: n=20, resp.). The incidence of bacterial and/or viral infection was significantly higher in LT-L group than in LT-H group (74.0 versus 8.5%: P<0.001). Occurrence of total viral infection in KT-L was also significantly higher than that in KT-H (36.8 versus 10%: P=0.046). The sensitivity and specificity of the IMK assay for identifying risk of infection was 0.810 and 0.878 in LDLT patients and 0.727 and 0.607 in KT patients. The percentage of LDLT patients with cytochrome P450 3A5 (CYP3A5) *1/*1 or *1/*3 genotype (expressors) was significantly higher in LT-L group than in LT-H group (53.8 versus 20.7%: P=0.032). In both LDLT and KT patients, the IMK assay can be useful for monitoring immunological aspects of bacterial and/or viral infection. CYP3A5 expressors in LT-L group are related to postoperative infections. |
| format | Article |
| id | doaj-art-c398cffec5174bfb9bd49491888a3e90 |
| institution | OA Journals |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
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| series | Clinical and Developmental Immunology |
| spelling | doaj-art-c398cffec5174bfb9bd49491888a3e902025-08-20T02:18:55ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/936063936063Immune Response following Liver Transplantation Compared to Kidney Transplantation: Usefulness of Monitoring Peripheral Blood CD4+ Adenosine Triphosphate Activity and Cytochrome P450 3A5 Genotype AssayYu Nobuoka0Shugo Mizuno1Kouhei Nishikawa2Kaname Nakatani3Yuichi Muraki4Tomomi Yamada5Masahiro Okuda6Tsutomu Nobori7Yoshiki Sugimura8Shuji Isaji9Department of Hepatobiliary Pancreatic and Transplant Surgery, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Hepatobiliary Pancreatic and Transplant Surgery, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDivisions of Nephro-Urologic Surgery and Andrology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Molecular and Laboratory Medicine, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Pharmacy, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanTranslational Medical Science, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Pharmacy, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Molecular and Laboratory Medicine, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDivisions of Nephro-Urologic Surgery and Andrology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanDepartment of Hepatobiliary Pancreatic and Transplant Surgery, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-0001, JapanSeventy living donor liver transplantation (LDLT) and 39 kidney transplantation (KT) patients were randomly screened by using the peripheral blood CD4+ adenosine triphosphate activity (ATP) assay (IMK assay). The patients were divided into 2 groups in each organ transplantation with low IMK ATP level (<225 ng/mL) or high (>225) (LT-L: n=23, KT-L: n=19, LT-H: n=47, and KT-H: n=20, resp.). The incidence of bacterial and/or viral infection was significantly higher in LT-L group than in LT-H group (74.0 versus 8.5%: P<0.001). Occurrence of total viral infection in KT-L was also significantly higher than that in KT-H (36.8 versus 10%: P=0.046). The sensitivity and specificity of the IMK assay for identifying risk of infection was 0.810 and 0.878 in LDLT patients and 0.727 and 0.607 in KT patients. The percentage of LDLT patients with cytochrome P450 3A5 (CYP3A5) *1/*1 or *1/*3 genotype (expressors) was significantly higher in LT-L group than in LT-H group (53.8 versus 20.7%: P=0.032). In both LDLT and KT patients, the IMK assay can be useful for monitoring immunological aspects of bacterial and/or viral infection. CYP3A5 expressors in LT-L group are related to postoperative infections.http://dx.doi.org/10.1155/2013/936063 |
| spellingShingle | Yu Nobuoka Shugo Mizuno Kouhei Nishikawa Kaname Nakatani Yuichi Muraki Tomomi Yamada Masahiro Okuda Tsutomu Nobori Yoshiki Sugimura Shuji Isaji Immune Response following Liver Transplantation Compared to Kidney Transplantation: Usefulness of Monitoring Peripheral Blood CD4+ Adenosine Triphosphate Activity and Cytochrome P450 3A5 Genotype Assay Clinical and Developmental Immunology |
| title | Immune Response following Liver Transplantation Compared to Kidney Transplantation: Usefulness of Monitoring Peripheral Blood CD4+ Adenosine Triphosphate Activity and Cytochrome P450 3A5 Genotype Assay |
| title_full | Immune Response following Liver Transplantation Compared to Kidney Transplantation: Usefulness of Monitoring Peripheral Blood CD4+ Adenosine Triphosphate Activity and Cytochrome P450 3A5 Genotype Assay |
| title_fullStr | Immune Response following Liver Transplantation Compared to Kidney Transplantation: Usefulness of Monitoring Peripheral Blood CD4+ Adenosine Triphosphate Activity and Cytochrome P450 3A5 Genotype Assay |
| title_full_unstemmed | Immune Response following Liver Transplantation Compared to Kidney Transplantation: Usefulness of Monitoring Peripheral Blood CD4+ Adenosine Triphosphate Activity and Cytochrome P450 3A5 Genotype Assay |
| title_short | Immune Response following Liver Transplantation Compared to Kidney Transplantation: Usefulness of Monitoring Peripheral Blood CD4+ Adenosine Triphosphate Activity and Cytochrome P450 3A5 Genotype Assay |
| title_sort | immune response following liver transplantation compared to kidney transplantation usefulness of monitoring peripheral blood cd4 adenosine triphosphate activity and cytochrome p450 3a5 genotype assay |
| url | http://dx.doi.org/10.1155/2013/936063 |
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