Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of β-lactam resistance
Introduction: Bovine mastitis is a frequent cause of economic loss in dairy herds. Coagulase-negative staphylococci (CoNS) are increasing in importance as causeof bovine intramammary infection (IMI) throughout the world in recent years. CoNShave been isolated from milk samples collected from cows wi...
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The Journal of Infection in Developing Countries
2015-09-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/5871 |
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| author | Mariela Elizabeth Srednik Mario Andres Grieben Adriana Bentancor Elida Raquel Gentilini |
| author_facet | Mariela Elizabeth Srednik Mario Andres Grieben Adriana Bentancor Elida Raquel Gentilini |
| author_sort | Mariela Elizabeth Srednik |
| collection | DOAJ |
| description | Introduction: Bovine mastitis is a frequent cause of economic loss in dairy herds. Coagulase-negative staphylococci (CoNS) are increasing in importance as causeof bovine intramammary infection (IMI) throughout the world in recent years. CoNShave been isolated from milk samples collected from cows with clinical andsubclinical mastitis in several countries. Identification of mastitis pathogensis important when selection appropriate antimicrobial therapy.Methodology: A total of 93 strains of Staphylococcusspp isolated from bovine clinical andsubclinical mastitis in Argentina during 2010-2013 were identified by Restriction Fragment Length Polymorphism (RFLP)-PCR using the gap gene. The isolates were tested by PCR for the presence of blaZ gene and mecA gene and were tested by disk diffusion for the susceptibilityto penicillin and cefoxitin. Results: The most common CoNS species was S.chromogenes 46.2% (43/93), followed by S. devriesei 11.8% (11/93) and S. haemolyticus 9.7% (9/93). The blaZ gene was detected in 19 (20.4%) but only 16 (17.2%) isolates were resistant to penicillin; the mecA was detected in6 (6.5%) isolates but only 4 (4.3) were resistant to cefoxitin. The 6 mecA-positive isolates showed oxaxillinMICs ≥ 0.5 μg/ml. Discussion: CoNSare important minor mastitis pathogens and can be the cause of substantial economic losses. Despite the low resistance to PEN in Argentina, the presenceof MR isolates found in this study emphasize the importance of identificationof CoNS when an IMI is present because of the potentially risk of lateraltransfer of resistance genes between staphylococcal species. |
| format | Article |
| id | doaj-art-c39155db83844ac8b8affa3bb738e128 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-09-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-c39155db83844ac8b8affa3bb738e1282025-08-20T02:16:14ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-09-0190910.3855/jidc.5871Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of β-lactam resistanceMariela Elizabeth Srednik0Mario Andres Grieben1Adriana Bentancor2Elida Raquel Gentilini3Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Buenos Aires, ArgentinaFacultad de Ciencias Veterinarias, Universidad de Buenos Aires, Buenos Aires, ArgentinaFacultad de Ciencias Veterinarias, Universidad de Buenos Aires, Buenos Aires, ArgentinaFacultad de Ciencias Veterinarias, Universidad de Buenos Aires, Buenos Aires, ArgentinaIntroduction: Bovine mastitis is a frequent cause of economic loss in dairy herds. Coagulase-negative staphylococci (CoNS) are increasing in importance as causeof bovine intramammary infection (IMI) throughout the world in recent years. CoNShave been isolated from milk samples collected from cows with clinical andsubclinical mastitis in several countries. Identification of mastitis pathogensis important when selection appropriate antimicrobial therapy.Methodology: A total of 93 strains of Staphylococcusspp isolated from bovine clinical andsubclinical mastitis in Argentina during 2010-2013 were identified by Restriction Fragment Length Polymorphism (RFLP)-PCR using the gap gene. The isolates were tested by PCR for the presence of blaZ gene and mecA gene and were tested by disk diffusion for the susceptibilityto penicillin and cefoxitin. Results: The most common CoNS species was S.chromogenes 46.2% (43/93), followed by S. devriesei 11.8% (11/93) and S. haemolyticus 9.7% (9/93). The blaZ gene was detected in 19 (20.4%) but only 16 (17.2%) isolates were resistant to penicillin; the mecA was detected in6 (6.5%) isolates but only 4 (4.3) were resistant to cefoxitin. The 6 mecA-positive isolates showed oxaxillinMICs ≥ 0.5 μg/ml. Discussion: CoNSare important minor mastitis pathogens and can be the cause of substantial economic losses. Despite the low resistance to PEN in Argentina, the presenceof MR isolates found in this study emphasize the importance of identificationof CoNS when an IMI is present because of the potentially risk of lateraltransfer of resistance genes between staphylococcal species.https://jidc.org/index.php/journal/article/view/5871bovine mastitiscoagulase-negative staphylococcimolecular identificationantimicrobial resistanceblaZ genemecA gene |
| spellingShingle | Mariela Elizabeth Srednik Mario Andres Grieben Adriana Bentancor Elida Raquel Gentilini Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of β-lactam resistance Journal of Infection in Developing Countries bovine mastitis coagulase-negative staphylococci molecular identification antimicrobial resistance blaZ gene mecA gene |
| title | Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of β-lactam resistance |
| title_full | Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of β-lactam resistance |
| title_fullStr | Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of β-lactam resistance |
| title_full_unstemmed | Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of β-lactam resistance |
| title_short | Molecular identification of coagulase-negative staphylococci isolated from bovine mastitis and detection of β-lactam resistance |
| title_sort | molecular identification of coagulase negative staphylococci isolated from bovine mastitis and detection of β lactam resistance |
| topic | bovine mastitis coagulase-negative staphylococci molecular identification antimicrobial resistance blaZ gene mecA gene |
| url | https://jidc.org/index.php/journal/article/view/5871 |
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