Lycii Radicis Cortex suppresses the growth of non-small cell lung cancer via enhancing the anti-tumor immunity

Lycii Radicis Cortex (LRC) is a medicinal and food homologous plant with various pharmacological activities, including anti-tumor effects. This study explores the anti-tumor effect of LRC on non-small cell lung cancer (NSCLC) and its molecular mechanism using mice bearing Lewis lung carcinoma cells....

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Main Authors: Heng Yin, Meng Liu, Yaling Zhao, Haitao Wu, Danna Zheng, Zhenhui Guo, Ying Zhou, Shaofeng Wu, Chuanbing Chen, Lei Zhang, Shanshan Song, Yanli He, Ren Zhang
Format: Article
Language:English
Published: Tsinghua University Press 2025-02-01
Series:Food Science and Human Wellness
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Online Access:https://www.sciopen.com/article/10.26599/FSHW.2024.9250041
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author Heng Yin
Meng Liu
Yaling Zhao
Haitao Wu
Danna Zheng
Zhenhui Guo
Ying Zhou
Shaofeng Wu
Chuanbing Chen
Lei Zhang
Shanshan Song
Yanli He
Ren Zhang
author_facet Heng Yin
Meng Liu
Yaling Zhao
Haitao Wu
Danna Zheng
Zhenhui Guo
Ying Zhou
Shaofeng Wu
Chuanbing Chen
Lei Zhang
Shanshan Song
Yanli He
Ren Zhang
author_sort Heng Yin
collection DOAJ
description Lycii Radicis Cortex (LRC) is a medicinal and food homologous plant with various pharmacological activities, including anti-tumor effects. This study explores the anti-tumor effect of LRC on non-small cell lung cancer (NSCLC) and its molecular mechanism using mice bearing Lewis lung carcinoma cells. LRC significantly suppressed the growth of NSCLC. Besides, RNA sequencing of mice tumors and hematoxylin & eosin and immunofluorescence staining revealed that LRC promoted the infiltration of T lymphocytes, specifically GZMB+CD8+T lymphocytes, in tumor tissues. The Gene Set Enrichment Analysis of spleen RNA indicated that LRC up-regulated PD-1-downstream pathways, suggesting that LRC exerted its effects through the PD-L1/PD-1 pathway. Further experiments revealed that LRC interacted with PD-L1, blocking PD-L1/PD-1 binding and thus restoring the T cell killing activity on tumor cells. Together, these results support using LRC as healthy food to improve anti-tumor immunity in patients with NSCLC.
format Article
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institution OA Journals
issn 2097-0765
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language English
publishDate 2025-02-01
publisher Tsinghua University Press
record_format Article
series Food Science and Human Wellness
spelling doaj-art-c38c475890e34ae4b794fe8654d6eedc2025-08-20T02:05:19ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302025-02-01142925004110.26599/FSHW.2024.9250041Lycii Radicis Cortex suppresses the growth of non-small cell lung cancer via enhancing the anti-tumor immunityHeng Yin0Meng Liu1Yaling Zhao2Haitao Wu3Danna Zheng4Zhenhui Guo5Ying Zhou6Shaofeng Wu7Chuanbing Chen8Lei Zhang9Shanshan Song10Yanli He11Ren Zhang12Research Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaResearch Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaResearch Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaScience and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaThe First Affiliated Hospital of Jinan University, Guangzhou 510006, ChinaResearch Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaResearch Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaResearch Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaIntegrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510315, ChinaDepartment of Pathology and Pathophysiology, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaResearch Centre of Basic Integrative Medicine, School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaLycii Radicis Cortex (LRC) is a medicinal and food homologous plant with various pharmacological activities, including anti-tumor effects. This study explores the anti-tumor effect of LRC on non-small cell lung cancer (NSCLC) and its molecular mechanism using mice bearing Lewis lung carcinoma cells. LRC significantly suppressed the growth of NSCLC. Besides, RNA sequencing of mice tumors and hematoxylin & eosin and immunofluorescence staining revealed that LRC promoted the infiltration of T lymphocytes, specifically GZMB+CD8+T lymphocytes, in tumor tissues. The Gene Set Enrichment Analysis of spleen RNA indicated that LRC up-regulated PD-1-downstream pathways, suggesting that LRC exerted its effects through the PD-L1/PD-1 pathway. Further experiments revealed that LRC interacted with PD-L1, blocking PD-L1/PD-1 binding and thus restoring the T cell killing activity on tumor cells. Together, these results support using LRC as healthy food to improve anti-tumor immunity in patients with NSCLC.https://www.sciopen.com/article/10.26599/FSHW.2024.9250041lycii radicis cortexnon-small cell lung cancerpd-l1immune checkpoint
spellingShingle Heng Yin
Meng Liu
Yaling Zhao
Haitao Wu
Danna Zheng
Zhenhui Guo
Ying Zhou
Shaofeng Wu
Chuanbing Chen
Lei Zhang
Shanshan Song
Yanli He
Ren Zhang
Lycii Radicis Cortex suppresses the growth of non-small cell lung cancer via enhancing the anti-tumor immunity
Food Science and Human Wellness
lycii radicis cortex
non-small cell lung cancer
pd-l1
immune checkpoint
title Lycii Radicis Cortex suppresses the growth of non-small cell lung cancer via enhancing the anti-tumor immunity
title_full Lycii Radicis Cortex suppresses the growth of non-small cell lung cancer via enhancing the anti-tumor immunity
title_fullStr Lycii Radicis Cortex suppresses the growth of non-small cell lung cancer via enhancing the anti-tumor immunity
title_full_unstemmed Lycii Radicis Cortex suppresses the growth of non-small cell lung cancer via enhancing the anti-tumor immunity
title_short Lycii Radicis Cortex suppresses the growth of non-small cell lung cancer via enhancing the anti-tumor immunity
title_sort lycii radicis cortex suppresses the growth of non small cell lung cancer via enhancing the anti tumor immunity
topic lycii radicis cortex
non-small cell lung cancer
pd-l1
immune checkpoint
url https://www.sciopen.com/article/10.26599/FSHW.2024.9250041
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